FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575550
Sequences flagged as poor quality0
Sequence length150
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA250094.345234992615759No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC229643.989922682651377No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA216463.76092433324646No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT191683.3303796368690817No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA186663.243158717748241No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC171052.97193988358961No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG165472.8749891408218224No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA148082.5728433672139692No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA139842.4296759621231865No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA136302.368169576926418No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG112731.958648249500478No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT110921.9272000694987401No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA98001.70271913821562No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT89211.5499956563287292No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG87721.5241073755538181No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT82661.4361914690296238No Hit
CCCCCTCCTCTGTTGCTGTAGGTTGGAAAGCCATAGGATTCCTGTAGAAC81471.4155155937798627No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT81401.4142993658239944No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG80501.398662149248545No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCGGGGC79901.3882373381982451No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG79801.3864998696898618No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC71291.2386412996264442No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG69661.2103205629397966No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA65051.1302232647033272No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT64541.1213621753105725No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT60511.0513421944227261No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT57771.003735557293024No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG55460.9636000347493701No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC53440.9285031708800278No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA52510.9123447137520632No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT51780.8996611936408652No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC50640.8798540526452958No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC46420.8065328815915213No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG41000.7123620884371471No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA38250.6645817044566067No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG33440.5810094692033707No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA32830.5704109113022326No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA30720.5337503257753453No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG25480.4427069759360611No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT24270.42168360698462337No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC23200.4030926939449222No Hit
CCCCCTCCTCTGTTGCTGTACGGTTGGAAAGCCATAGGATTCCTGTAGAA21700.377030666319173No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC21270.3695595517331248No Hit
AGCATCTTATCCGAGTGGAAGGGTAGCAGTAGGGGGGCTTTCTCTGTAGA18180.3158717748240813No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGACTCCACACCCC17620.3061419511771349No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT17530.30457822951958996No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC17350.30145078620450005No Hit
CTTGGGCCTGTGTTATCTCCTGTCCCTTCCCAGAAAACCTACCAGGGCAG15250.26496394752845104No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT14930.2594040483016245No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA14880.2585353140474329No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA14490.2517591868647381No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA12740.22135348796803056No Hit
CCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA11160.19390148553557468No Hit
TCTGGATCAGAGTCAGTGGTGGGGGTTGTCCACGCTGTAGAACCATGTCG11120.19320649813222138No Hit
CTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT10920.18973156111545478No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGGGCAGCTACGGTTT10610.1843454087394666No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC10350.17982799061767005No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGACGGTACAT10170.17670054730258014No Hit
TCCTTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA9200.15984710277126227No Hit
CTTGGGCCTGTGTTATCTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC8960.1556771783511424No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG8960.1556771783511424No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG8770.15237598818521414No Hit
CCTGGTCCTCTGACTGCTCTCAGAATGCCAGAGGCTGTGTAGAACCATGT8740.15185474763269916No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT8720.15150725393102252No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA8370.145426114151681No Hit
CTTGGGCCTGTGTTATCTCCGACCCCTGTAGAACCATGTCGTCAGTGTAG8260.1435148987924594No Hit
CTTGGGCCTGTGTTATCTCCCCTGTAGAACCATGTCGTCAGTGTAGATCT7000.12162279558683No Hit
AGAATGGTCCTGCACCAGTAATATGCGTATTAAAACAAGATTTACCTCTA6960.12092780818347666No Hit
CCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG6790.11797411171922509No Hit
CTTGGGCCTGTGTTATCTCCTGTAGAACCATGTCGTCAGTGTAGATCTCG6760.1174528711667101No Hit
TCCACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC6760.1174528711667101No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA6320.10980800972982364No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG6120.10633307271305707No Hit
AAGACCCAGGTCCAGATGATTCCTTAACAGGGCAGCTACGGTTTCCTGTA6010.10442185735383545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)