FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875963
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG363724.152230174105527No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCGAATGTGCAT315043.5964989388821214No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC238352.721005339266613No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA226932.5906345359335954No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA216062.4665425366139893No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG190292.172352028567417No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT190252.1718953882755323No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA185482.1174410334683085No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC182122.079083248950013No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT175021.9980295971405184No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG161241.8407170165863171No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA157811.801560111557223No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT144081.6448183313678775No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT130621.4911588731487517No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG129251.4755189431517084No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTGGGTG126651.4458373241792177No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC119481.3639845518589255No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC114071.3022239523815502No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT108761.2416049536338865No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG108521.2388651118825795No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA108471.238294311517724No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC102661.1719673091215042No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT100561.1479936937975692No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT96671.1035854254118038No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG95331.0882879756336739No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA94921.0836074126418582No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA92771.0590629969530676No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC91441.043879707247909No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA89731.0243583347698477No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG88081.005521922729613No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT78910.9008371358150972No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA77370.8832564845775449No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC76610.8745803190317399No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT76350.8716121571344909No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG72530.8280030092595236No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG71730.818870203421834No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG69660.7952390683168125No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG68360.7803982588305671No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC63090.7202359003747876No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATCGAATGTGCATCTCG62680.7155553373829716No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG59940.6842754773888852No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT53390.6095006295928024No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT52050.5942031798146725No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG49100.5605259582881925No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC48450.5531055535450699No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA48270.5510506722315898No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG42740.487920151878561No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA42360.48358206910565854No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT40380.46097837465737707No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC37830.43186755604974186No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC37410.42707283298495485No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG31190.35606526759691903No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC30200.3447634203727783No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC28660.32718276913522604No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATCG24260.2769523370279338No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT22470.2565176839661036No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT19580.2235254228774503No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG19270.2199864606153456No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT18410.2101686943398294No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG18200.20777133280743595No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCGAATGTGCAT14900.17009850872696677No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG14100.1609657028892773No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT13380.15274617763535675No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTGGGGGTG12570.14349921172469615No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTATCGAATGTGCAT12260.13996024946259147No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTATCGAATGTGCATCTCG11290.12888672238439294No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA11210.12797344180062398No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG9610.10970783012524501No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC9300.10616886786314034No Hit
ATACGGCCAGGCATTGAAGTTTCATGGAAGCCAGCCCCTCAGGGCAACTG9020.10297238581994901No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGAGTGGGAGGCTG8820.10068918436052664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)