FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935486
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATAGCTTCACTAT352583.7689500430792124No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA267482.8592624582302677No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC250752.6804249342053224No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA247442.645042256110728No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA229912.4576530274103514No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG223452.3885980121562485No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG217882.3290567683535617No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC208482.2285742384172504No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG190032.031350549340129No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT183801.964754149180212No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA168371.7998131452528419No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA161601.7274443444370093No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC153471.6405376456729441No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG138951.4853242058138765No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT125861.345396938062141No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA119071.2728143446294227No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC114511.2240696279794674No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA110771.1840904086218287No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA106831.1419732630953323No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA103581.107231962851395No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG101191.0816837451335457No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC97851.045980378113622No Hit
GACCCAAAACCCAAAATGGCTACGGTAGGAGACAGAAGCAGGGAAGACCA96431.0308011023147328No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC90220.9644184947717016No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG87280.9329909800894937No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG86940.9293565056024355No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG83660.8942945164331695No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT81110.867035957780234No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT79300.847687725952072No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG77500.8284463904323528No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC76080.8132671146334634No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT74290.7941326754221869No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG73180.7822671851850269No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT73090.7813051184090409No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG62450.667567446225812No Hit
TCCACAAACAGAACAAGATGCTGCTACCGTAATCCTGTTTGAAGGAAAAG62280.665750208982283No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT56380.6026813870009813No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG53780.5748883468058315No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC52780.5641987159615429No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT51860.5543642555847976No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG51850.5542573592763548No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT51460.5500884032470823No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATAGCTTCACTATCTCG51030.5454918619840382No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG50800.5430332468898519No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT49180.5257160449221047No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT39580.4230955888169358No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC35200.3762750057189525No Hit
TGTTCATTCATGATCCCACTGCTACCGTACAAGTGTGAGTGACTGGTGGA35090.37509914632608077No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA33930.3626991745467062No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC32090.3430302537932155No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTATGTGGGGGTTG28930.3092510203252641No Hit
TCAAATCATCCATTGCTTGGTACGGAGCAGAAAGTCAGTCCCATGGAGAC28160.30102000457516204No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATAG27770.2968510485458895No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT27760.29674415223744666No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC26910.2876579660198014No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT26330.2814579801301142No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT25180.2691649046591825No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC24970.2669200821818819No Hit
AGCTGCTCACCATCGCTACGGTAGCAGGTCTTGGCCAGTTGGAGACCAAG22000.2351718785743453No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG21450.2292925816099867No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTATAGCTTCACTATCTCG20610.22031329170078442No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT20260.21657192090528346No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT19810.21176158702535366No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGCC19530.2087684903889529No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATAGCTTCACTAT19380.20716504576230965No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTATAGCTTCACTAT18370.19636851860957835No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATCTTCTTTCTCTAGGTG15390.16451341869359884No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA14650.1566030918688254No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATAGC14630.15638929925193965No Hit
CAGAGGGGAAAAATATGACAAAGTCTCTGCTACCGTAGTGCTAAAATGGA14060.15029620967069524No Hit
TGACCAATGGCTAAGTGAAGATGTTTTTCCTTTCCTCTTCCTGGAAGACC14020.1498686244369237No Hit
AGATGAGTCATATTTGTGGGTTTTCAGAAGACCACTGACTCTGATCCAGA13730.14676863149208005No Hit
TCATAATGCTTGCTCTGATAGGAACACTGACGACATGGAGTGGGTCCCAT13450.1437755348556793No Hit
TGTGCTGTGACTGCTTGTAGGGTAGCACAGAGTTGAAAGTCAGGGCAAGA13120.1402479566770641No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG12520.13383417817049106No Hit
GTAGAGGAGCCGTCAAATCCATGAAAGACCAAGTCTCTGCTACCGTATAG12480.13340659293671953No Hit
GGAATCCTATGGCTTTCCAACCTGACGACATGGTTCTACAGCAACAGAGG11790.1260307476541605No Hit
TCCCTGCCAGCGAGATGGATGAGGCCTTTGCATCCCTAGACCAAGTCTCT11330.12111351746578783No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATAGCTTCACTATCT11210.11983076176447323No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT11010.11769283559561554No Hit
GGGGTCAGAGGCAAGCAGTCACAGCACATGTAGAACCAGGAGATAACACA10400.1111721607805996No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACAAGCTGTGTCTACTCATTTG9970.10657561951755558No Hit
TGTGCTGTGACTGCTTGTAGAGTAGCACAGAGTTGAAAGTCAGGGCAAGA9900.10582734535845541No Hit
GCCTGCTGAAAATGACTGAATATAAATTTGTGGTAGTTGGAGCTGGTGGC9820.1049721748909123No Hit
ACTTCTACCGTGCCCTGAAACAGAGAAGACCTGTAGAAAGCTGTGTCTAC9770.1044376933486979No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA9650.10315493764738329No Hit
GCAACAGGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATAGCTTCACT9630.10294114503049752No Hit
CCCCTGCTCCTATAGCCAAGTCTCTGATGCCGACGAGTACCTCATAGACC9460.10112390778696848No Hit
GCAACAGATGAGGGGGAGACCAAGTCTCTGCTACCGTATAGCTTCACTAT9390.1003756336278683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)