FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935486
Sequences flagged as poor quality0
Sequence length150
%GC42

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG353293.776539680978657No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC233312.4939977722809323No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG225162.406877280899981No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA216612.3154809371813156No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA213172.2787086070769633No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC204312.183998477796568No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA196832.1040400390812906No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT177151.8936681040656944No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA171891.837440645824737No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT169561.812533805957545No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA137081.465334596135057No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT128741.3761830748936916No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT116831.2488695715382163No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA111671.1937110763816883No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT111621.193176594839474No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG110831.184731786472486No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC98711.0551734606397103No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA95281.0185080268438012No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT94291.0079252923079554No Hit
TCCCTGCTTCTGTCTCCTACCGTAGCCATTTTGGGTTTTGGGTCTGTAGA93500.9994804839409677No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG93490.9993735876325247No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT91870.9820563856647775No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT90500.9674115914081023No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC90230.9645253910801445No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG87590.9363047656512229No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC76380.8164740038867498No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG76240.8149774555685494No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA75280.8047154099580326No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA73580.7865430375227421No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT73290.7834430445778985No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG72760.7777775402304257No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG71780.767301702003023No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG68140.7283914457298132No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC63630.6801812106220724No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCAGCATCTTGTTCTGTTTGT60180.6433019842092773No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT59370.6346433832254037No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG52090.556822870678984No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC51480.550302195863968Illumina Single End PCR Primer 1 (100% over 21bp)
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT51060.5458125509093669No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT50960.544743587824938No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC50850.5435677284320664No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT49910.5335194754384353No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA47550.5082919466459146No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC47200.5045505758504136No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA37340.3991508157257297No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA35640.38097844329043945No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT35270.37702327987805273No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG34540.36921984936172214No Hit
CCACCAGTCACTCACACTTGTACGGTAGCAGTGGGATCATGAATGAACAT34140.36494399702400676No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA31800.33993026084837186No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG28690.3066855089226349No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT27690.29599587807834643No Hit
CCATGGGACTGACTTTCTGCTCCGTACCAAGCAATGGATGATTTGATGTA27650.2955682928445749No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA27180.29054416634775937No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA27160.29033037373087356No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT25890.2767545425586273No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT25420.2717304160618117No Hit
ACAAGTCAACAACCCCCACATACGGTAGCAGAGACTTCCATTTTCTACTT24910.26627870433122464No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTTTTT23600.2522752879252068No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC23540.2516339100745495No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT23330.24938908759724893No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG23290.2489615023634774No Hit
CCAACTGGCCAAGACCTGCTACCGTAGCGATGGTGAGCAGCTTGTAGAAC21520.23004085576908687No Hit
CCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC20910.22352018095407092Illumina Single End PCR Primer 1 (95% over 22bp)
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT20660.2208477732429988No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG19610.20962366085649597No Hit
CTTGGGCCTGTGTTATCTCCTGCTTGCCTCTGACCCCTGTAGAACCATGT19340.20673746052853811No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG18460.1973305853855643No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG16800.17958579818404552No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA16710.17862373140805954No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT15510.16579617439491345No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT14360.15350309892398176No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC14130.1510444838297954No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA14060.15029620967069524No Hit
TCCAGGAAGAGGAAAGGAAAAACATCTTCACTTAGCCATTGGTCATGTAG13750.1469824241089658No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGACAGAGATTTCATACC13620.14559277209920832No Hit
AACAGAGAATCTCCATTTTAGCACTACGGTAGCAGAGACTTTGTCATATT13520.1445238090147795No Hit
CTGATGGGACCCACTCCATGTCGTCAGTGTTCCTATCAGAGCAAGCATTA13010.13907209728419237No Hit
TCTGGATCAGAGTCAGTGGTCTTCTGAAAACCCACAAATATGACTCATCT12990.13885830466730661No Hit
TGCCCTGACTTTCAACTCTGTGCTACCCTACAAGCAGTCACAGCACATGT12840.13725486004066337No Hit
AGGGATGCAAAGGCCTCATCCATCTCGCTGGCAGGGATGTAGAACCATGT12630.13501003756336277No Hit
CCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT12600.13468934863803414No Hit
TTCATGGATTTGACGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA12110.1294514295243328No Hit
CCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA11600.12399971779374572No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT11510.12303765101775975No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGGTTGGAAAGCCATAG11400.12186179162488803No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC11270.12047213961513054No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAGTATCTATAATGATCAGGTTCATT11100.11865490237160149No Hit
CTTGGGCCTGTGTTATCTCCTGGTTCTACATGTGCTGTGACTGCTTGCCT10490.11213422755658554No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC10420.11138595339748537No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC9960.1064687232091127No Hit
GTTCAAATGAGTAGACACAGCTTGTCTTCTCTGTTTCAGGGCACGGTAGA9700.1036894191895977No Hit
TGCCCTGACTTTCAACTCTGTGCTACTCTACAAGCAGTCACAGCACATGT9690.10358252288115483No Hit
CCTCCTGGCCCCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGT9640.1030480413389404No Hit
GTTCAAATGAGTAGACACAGCTTTCTACAGGTCTTCTCTGTTTCAGGGCA9630.10294114503049752No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTACAACA9610.10272735241361175No Hit
CCCCCTCATCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG9450.10101701147852561No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT9450.10101701147852561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)