FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370946
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC113923.071066947749807No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC95162.565332959514323No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA92172.484728235376578No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA89192.404393092256016No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT84742.284429539609539No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTC84032.265289287389539No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT81802.205172720557709No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC80502.170127188323907No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC79372.1396645333822173No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA75142.025631763113769No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT72151.9450270389760234No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT71621.9307392450653196No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA67751.8264113914154623No Hit
CCTGCCCTGGAGGAAGACAGTACAGCATCACACCCACGCTGAGAGTCGTC63341.7075261628377174No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA63231.7045607716487037No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA61521.6584624177103946No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA60171.6220689803906767No Hit
GTTGTTTCAGAACACCGAAAAGCAATACCATCCACTTGTAAACCCAGGGA59451.602659147153494No Hit
TTTGCTAATAGATGCCTAAGCCCAGAAAGGGTGCTTCTTCAACTAAAATA50311.356262097448146No Hit
ATTTACCCTACCAATTGCCCAGTGCGGGACTACCAGCTGCACATTTCCCG50231.3541054493106812No Hit
TCGGGGGCCTACAAGAGTCCCACCAAGAGGGGGAAGAGGGAGAGGAGTTC47851.289945167221105No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG47691.2856318709461756No Hit
GCTCCAAACAAGATGATAGCCCCTCGGGAGCTTCTTATGGCCAAGATTAT47451.279161926533781No Hit
CTACCACTGGCCACTGTAACAGTGGACGAACTCGCCACGGATCCGCAAGC47211.272691982121387No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT43691.177799464072938No Hit
TGGATCCTTTATTACGAATTGTGATCACATCCTCTGATTGGCAACATGTT43411.1702511955918113No Hit
CTGACACTTACAACAGTCAAGTTCTGATAGAAGCTACAGTAATAGCACTA42511.1459889040453328No Hit
CGGCAGTTGTGTCTAGAGAATGGCGGGTTCTGCACCTCCGCGGGCGCCCT40991.1050125894335026No Hit
CTTGAGGCATTGTTATTTCAGCATTCTTTTTTTTTTCTTGCTTTTAATTT37801.0190162449520954No Hit
CACGTTTTTCCCATCTAGCAATTCCATGCACTTCTCATTTCTGTTGAAGT37731.017129177831814No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCTACAATACGGAC36150.974535377116885No Hit
GCGTCTTCACCTGGAAGGGCACCTGTGACAGCCTATCCTTGCTGCAACTT34790.9378723587799842No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT33490.902826826546182No Hit
AGATGATACTGCTGCCCTCGGACTGCCGAGGACACAAAGCCAGGCCTAAA31820.8578067966766053No Hit
GCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGACAGAG31550.8505281092126618No Hit
ACCCGAACGTGATGAAAAGATCAAAGAACCTACTCTGTTGGGTTTTCATA30870.8321966000442114No Hit
TCTATTGTGATGGCCACGTTTTCCATTATTTTCTAATGGCAAAGATTTCT30280.8162913200304087No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA30150.8127867668070285No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG27320.7364953389442129No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT25630.6909361470402701No Hit
CTTGAGGCATTGTTATTTCAGCATTCTTTTTTTTTCTTGCTTTTAATTTT25470.6866228507653405No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACGC25200.6793441633013969No Hit
CAGTAAATCATAGTCTTTCCTTCATAAGTGACCACATTTAGCTGGGACAC24930.6720654758374535No Hit
TTGGTTGTTTGTCATCTTTCTGTTGACAGTATGCAGTTGGCAGTAGATGT24230.6531948046346369No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGGTACATGT23540.6345937144490034No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT22260.6000873442495673No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC21350.5755554716859058No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG20690.5577631245518215No Hit
TCTCCACTTCACCCCGTCACCACCACTTTCCAGGTTGGTTCTACTGCTGT19520.5262221455413996No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA19240.5186738770602729No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC18560.5003423678918225No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACGCGTAT18230.4914461943247805No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC17600.4744625902422455No Hit
TGTTTTTGTAGGTAATTTTAACCAATCAGATGACAACAAAGATTGATAGA17300.4663751597267527No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA17040.45936605327999225No Hit
TGTACTTGAAAAAGGACTTTAGAGCAAGCTTAAACTAGATTGCTTTTAAA16890.4553223380222458No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT16630.44831323157548536No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC16600.4475044885239361No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA15830.4267467502008379No Hit
GTGCTCAAAAGAAGTGCTGAAGGAAGCAACCCTCCTAAACCACTGAAAAA15680.42270303494309147No Hit
CAGCATCCTGCCCTTACCTGGGTGCGGAAGAGAAAGACCAAGTCTCTGCT15340.4135372803588663No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATGTAAGTCCAAAAGG15090.40679775492928893No Hit
AAGTCCTCCATTTCTGTATCCATGCGTTTAGGACTCAGTTCCTCTGGAAA14580.3930491230529511No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACGCG14570.39277954203576804No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACG14230.38361378745154284No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC13200.35584694268168415No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC12620.34021124368506467No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT12110.3264626118087269No Hit
GTTAACTGCTACTAAAACGGAGCAAAATATAAAAGATTTTGAGACTTCTG12020.324036382654079No Hit
TGACAGTTTGACAGTTAAAGGCATTACGGTAGCAGCATCTTGAATCTCAT11990.32322763960252976No Hit
TTCAGGTGTATTTTGGAGGCCAAGGCGGGTGAATCTCATTGAGCTCAGGT11950.32214931553379733No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACGCGTA11770.3172968572245017No Hit
GCCAAACGAAAATTATGGCAGGTACACTGACGACATTCAGAGTGAAGAAA11590.312444398915206No Hit
AGGACGAAAACTGGACATTCTCATTAAGGATAATCAACTTCGGGTGTGAG11060.298156605004502No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCAAGGTAATTAAATG10320.27820760973295305No Hit
TCTATGCTTGTTTCCCGACTGTGGTTAACTTCATGTCCCAATGGATACTT10280.2771292856642207No Hit
ATTCATGGGTTGCAAGTAGAAGTTTGTCCTCTTAATGGCTGTGATTACAT10130.27308557040647424No Hit
CCAGGGTAGGTCAAAAGTATCCTTTGATTGGAAAAATCTAATGTAATGGG9830.2649981398909815No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTTATACTTTAACAGGATTTG9720.2620327487019674No Hit
AACTGAGCGACCTCAGAGCCCTGGCAGCTCCAAGGCGCCTTTGCCAGCAG9550.2574498714098548No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG9220.24855369784281273No Hit
GGAAACACTGATGGGCCTGAAACAGGGTTCAGTGCCATTGACACTGATGA9170.24720579275689722No Hit
TGCAGGGCTGATTGTCTTCTTTTAAGGTAAAATATATGGAGCAGGTATAA8870.23911836224140443No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG8780.23669213308675657No Hit
GTTACTCTGTTTGATTCTCGGCATTTACTCTACCAACTGCTCTGGAACAT8750.23588339003520728No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT8460.22806554053689757No Hit
GTGTTTGTTCCAATACAGCAGATGAAATATTACCTAGATCTTGCCTTGGC8450.22779595951971446No Hit
ACTCTACCCTGCGATTTTCTCTCATACAAAGATCTGAATCTCTAACTTTC8370.2256393113822497No Hit
CACCTCGAACACCCAGGCACACTGACGACATGGGTTCTATGATTTCAGTG8300.22375224426196805No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT8250.22240433917605257No Hit
ATAATGATGATGCCAAACGACAAAGAGTTACTGCTATTCTTGACAAGCTG7980.21512565171210907No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACGC7840.21135151747154574No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA7140.19248084626872913No Hit
GCTGGTACTGAGCAAGAAGACAAAATTTCCATTTACATGATGAGCTTACC7100.19140252219999676No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG6950.18735880694225035No Hit
GGTATTTAAAGGCTTTTGTTTTCTGTTGGGGTTTTTATAGAACCTGCTTT6840.1843934157532363No Hit
CTTTCTTCAGAAGCTCCACCCTATAATTCTGAACCTGCAGAAGAATCTGA6830.18412383473605323No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG6650.17927137642675753No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACGCGT6350.1711839459112647No Hit
CTTGAGGCATTGTTATTTCAGCATTCTTTTTTTTTTTCTTGCTTTTAATT6090.16417483946450429No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG6050.16309651539577188No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC5720.1542003418287298No Hit
ATGCAATTCAAATAAAAACGAAACTGTGTCAATTAGTTGAAGTAATGATG5650.15231327470844813No Hit
GTGGTGGTATACGATATGGGTTTTGTAAAAGTCCATGTTTATTTGGAGTA5610.15123495063971576No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT5250.14153003402112438No Hit
GACTACAGATGTGCCACCATACCAACTGCTCTGGAACATGTTAGACCAAG5090.13721673774619486No Hit
CTCCTAATTGTGAGATATATTATCAAAGTCCTTTATCACTTTGTATGGCC5050.1361384136774625No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA4840.13047721231661752No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT4560.12292894383549087No Hit
ATCCTGTTCCAAGTCAAGACCACATTTAGCTGGGACACATACTTCTCTTA4420.11915480959492757No Hit
CTGGCTGAGCACATAGAGCATGAACAACAGAAACTACCTGCTGCCACCTT4210.11349360823408258No Hit
TTCAGGTGTATTTTGGAGGCCACTGCTATGGCTTATGAAAATAATTGTTT4030.10864114992478689No Hit
AGATGAGTCATATTTGTGGGTTTTCATACAGCTTCCCTCTTTAAGATGTC4030.10864114992478689No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTACGCGTATCATATC3980.10729324483887144No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG3960.10675408280450523No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTATCACTGGGTCAAAAACTA3960.10675408280450523No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACGCG3930.10594533975295595No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC3750.10109288144366027No Hit
TGTCCAGTCTATCATTCATATCCGGACCCGCTGGGAACTGTCACAGCCCG3740.10082330042647718No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)