FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370946
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG114133.076728149110652No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA95042.562097987308126No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG88712.3914532034312272No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA87642.362608034592636No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT84082.2666371924754545No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC82932.235635375499399No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC81512.1973548710593995No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG74922.0197009807357404No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT74522.0089177400484166No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT71411.9250780437044746No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC70371.8970416179174328No Hit
TATGGTCCTCAGAAAAGTGCACCAAACCAGATTCTAGCAGTCCCATTAAG67141.8099669493672934No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC65951.7778868083225052No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA65081.7544332598275758No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT64561.740415046934055No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG62311.679759318067859No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT61361.654149121435465No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA60181.62233856140786No Hit
ACATACTCTTGACAGTCTATTTGGGATATTTATTTTTCTCCGAAATTAGG57621.553325821008988No Hit
ACCAAATTTTCCCTGGGTTTACAAGTGGATGGTATTGCTTTTCGGTGTTC56831.5320289206515234No Hit
GTCACTGACAACTGGCTTGTGCAACATTTTGACATGGAAGTCACAGACTA49541.3355043591250477No Hit
CTGTGTCACCAAGGGTTTCGTTGGGGCCATGAAGACCACCTTTCCTGAAG48521.3080070953723721No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG47511.2807794126368799No Hit
AATGCAGATGATTACCTCGCTCTGGCAAATGATTTCATTAAAGATTCAGA45911.2376464498875848No Hit
GGCAAGGACAGGGAAGGGGTCACTAGTGAAGAGCCCATGTTAAGTTGATT45861.2362985448016692No Hit
TACTGTAGGGGCTCGGTGACCGGGTGCTGGACTGGTAGGCCGAAGGCTCC45021.2136537393582894No Hit
TGAAAGAAAGTAACATTCAACACTGATACCCAAAATGAATGCACTCATTT40001.078324068732376No Hit
ACCAGCGGTAGAAATTGTACATGACCACGGCGGCAATAAAGGTCTTTCCC37841.0200945690208278No Hit
CACGCACACACAAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACG37621.0141637866427997No Hit
CCAGATCCTTAACATTGGTCCGTATTGTAGAACCATGTCGTCAGTGTACT37381.0076938422304056No Hit
CTGGGATAAAAGTTGCAGCAAGGATAGGCTGTCACAGGTGCCCTTCCAGG34450.928706604195759No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA33230.8958177200994215No Hit
CGACTCAGAGACCGCTTGCTCAGGAAAGGGCTGCTGGACCGCCTTCGACC30960.8346228291988592No Hit
GACGGCTGCTCTTTTCGTTCTGTCGCCTGCCCGATGGACGAGCCTCCCGG30360.8184479681678736No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT30120.8119780237554792No Hit
GGAAGTTTAACTGAAGTCTCTTTACCATTGGCTGAGCAAATAGAGTCTCC30120.8119780237554792No Hit
TAATGATCAAGGGGAGAAGGTGCCTTGCTTCATGCAGTGTTAGTAAAACA29280.7893332183120993No Hit
ACTTTAGGGTCTTTGCCCATTGATGGCTAAAACTGGTGATTTCACTAGTA29260.7887940562777331No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG26190.7060326840025233No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT25660.6917448900918193No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA25270.6812312304216787No Hit
ATGTGTCCCAGCTAAATGTGGTCACTTATGAAGGAAAGACTATGATTTAC23620.6367503625864681No Hit
ACCCTCTCCCTTGTGTTTTTCTGCTATAAGCTGAAGGTGCTGAATGCAGG21440.5779817008405537No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG21340.5752858906687227No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG20640.5564152194659061No Hit
GCAATGGTTAAGGTGAACTGGTTCTCATCTACTAGGCAGATTTCTTCAAT19180.5170563909571744No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG18510.49899446280590704No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG18430.4968378146684423No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC18070.4871328980498509No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG17780.4793150485515412No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC17750.47850630549999196No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT16540.4458870024208375No Hit
AATCAAATGAAAGAGATAAGAAAAACTGTGATAAACTAAAATGTTTATCT16520.44534784038647135No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT16000.4313296274929505No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT15940.42971214138985186No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA15880.4280946552867533No Hit
CCTACCACAATATTCGGAGATGGGTTTTTCCTAAACTGATCTCTTGCCAG15870.4278250742695703No Hit
TTCTCTTCCGCACCCAGGTAAGGGCAGGATGCTGTGTAGAACCATGTCGT14920.4022148776371763No Hit
AAACTTGGCATGAAAGAAATTGGTATAAGCCAGGAGCAGTGCTGAGACTC14880.4011365535684439No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT14530.3917012179670356No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG14190.3825354633828104No Hit
ACCTGATGAAGACTTTGGACCTCTTAAGCTTGAAAAAGTGAAGTCCTGCT13620.3671693454033741No Hit
TCCTATTTCATACATCTAAATTCAGAACCTTTTTGAAAACCAAGAGTGCA13420.3617777250597122No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCGTCAGTGTACCTGCCATAATTT13290.358273171836332No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG12940.3488378362349237No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA12590.3394025006335154No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT12110.3264626118087269No Hit
GTATGAGATTCAAGATGCTGCTACCGTAATGCCTTTAACTGTCAAACTGT12080.32565386875717756No Hit
GCACCTGAGCTCAATGAGATTCACCCGCCTTGGCCTCCAAAATACACCTG11910.321070991465065No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT11850.31945350536196643No Hit
AAGGAAAAGTTATGCAATTCTTCTGGTTTCTGATCAAAGAAATTTACAAT11320.3051657114512624No Hit
TGCAATTTGGTCTCTGCATGTACCGCAGAATTGCTGCTTCCCTCAGCCTT10750.28979959347182604No Hit
ATGTGTCCCAGCTAAATGTGGTCTTGACTTGGAACAGGATGCCCCTGAAG10440.28144258193915017No Hit
GGAAACTTGTAAGGGCTTCGAGGAATGTGAGGTATTGGTGACAAGGTAGG9860.2658068829425307No Hit
GGCACTCAGGAAAGTATCTCGTTACTGGAAGTTAGCACTCTAGGGAAGGC9660.2604152625988688No Hit
CTGTCCTTTTCCACAATCACACATATTCTTTCAAACATACTCTGCAGGTG9660.2604152625988688No Hit
TGCACGTACCACATTTAATTACCTTGACTTGGTCTTCTCTTGGTATTAGT9470.25529322327239007No Hit
CACTGGGCAATTGGTAGGGTAAATCCACAGGGCGCCCGCGGAGGTGCAGA9160.2469362117397141No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT8970.24181417241323533No Hit
ACAACTGTTTCATATACTTCATCTTCTAGGACATTTGGCATTGACTTTCC8790.23696171410393962No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG8610.23210925579464398No Hit
GCTAACATGTTGCCAATCAGAGGATGTGATCACAATTCGTAATAAAGGAT8550.2304917696915454No Hit
TCCCCTGGAAACTGCACTGACCCTGTGCCCTGGTAACTGCTGGACTCCCC8550.2304917696915454No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA8460.22806554053689757No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT8290.22348266324478494No Hit
TTGACATACCTCAGCACACACATACACACAAAATGAATGATACAAACCTT8270.22294350121041875No Hit
AAGACATCACTGAAATCATAGAACCCATGTCGTCAGTGTGCCTGGGTGTT7930.2137777466261936No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA7800.21027319340281336No Hit
ACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAA7760.209194869334081No Hit
TGGGAGGTGAGAGTTTAATAAAATAGTCAAGGACACATAGCATTAACTGA7680.2070382211966162No Hit
ATGTGCACAAAGGAGATACTAGATAAATATTTGAGCAAACTCAATACCTG7650.20622947814506695No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC6890.1857413208391518No Hit
GAGATTGGTACAGCGGCAGAGTCAGCCCTTGTTCTTTGAATATTATTGGA6680.18008011947830682No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT6660.1795409574439406No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA6620.17846263337520823No Hit
ACAGACTTACTCCTGGACAAATCAGATTGATATTTCAGACAAAAATGGGT6620.17846263337520823No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC6420.17307101303154637No Hit
AAATGATCAAGGGGAGAAGGTGCCTTGCTTCATGCAGTGTTAGTAAAACA6390.17226226997999708No Hit
AAACCAATGCTCTCACCTTAAAGTGTTGGTTGTTGTGAGGGCTTATACGA6170.166331487601969No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT5960.16067028624112406No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA5700.1536611797943636No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT5410.14584333029605387No Hit
TTCATGTCCTCCTTTCTACCAATAACCGCATATCTACTCTTTTTCTGTAC5300.14287793910703983No Hit
AGACAGTTAATATCACTGCAGGCTTTCCTGTGGTTGGTCAGAAAGATAAG5250.14153003402112438No Hit
AACATGTTCCAGAGCAGTTGGTATGGTGGCACATCTGTAGTCTGTAGAAC5200.1401821289352089No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC4560.12292894383549087No Hit
AGAAATCCAAGGCTCTTCTCTTTTTGCAGTTCTTTTGGTCATCAATCTCT4330.11672858044027971No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT4310.11618941840591353No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT4200.11322402721689949No Hit
TCACAGCCATTAAGAGGACAAACTTCTACTTGCAACCCATGAATTGCTCT4110.11079779806225165No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT3940.10621492077013904No Hit
GGCCAGGATATAGTCTAGTTAGTCAAGAAAAGCAATGAATCGTTTACAAA3930.10594533975295595No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG3820.10297994856394192No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT3770.10163204347802646No Hit
TCATGATACTAGTTTTTGACCCAGTGATACGGTAGCAGATACATTGGACC3730.10055371940929407No Hit
AGAGACATCTTAAAGAGGGAAGCTGTATGAAAACCCACAAATATGACTCA3720.10028413839211098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)