FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96275
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC1080811.22617501947546No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC966010.033757465593354No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC87059.041807322773305No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG78538.15684237860296No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC50815.277590236302259No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT33033.4307971955336276No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA30183.1347701895611526No Hit
TACTCTTCTTGGCTCCAGTTGCCACTATTTCTTCCCATTCACCGAGCCCC30073.1233445858218642No Hit
CGGCCCCTTCCGCTTTACACGAATGGTTCTACACGGCCCCTTCCCTGTCC24832.5790703713321217No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA21742.2581147753830173No Hit
GCCTACCAGTCCAGCACCTACACAGTGCAGTTTCCAGGGGAAGACCAAGT19372.0119449493638015No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA18421.91326928070631No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA16151.6774863671773566No Hit
CAAAGTGGGAAGGGGAGTGAATATAAGCGTGAATGTAAGCGTGAACATCG15081.5663464035315502No Hit
ATCCTGTTCCAAGTCAAGACCACGTCTCTGCTACCGTAAGTTTGTCCTCT14951.5528434172942094No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGTG13341.3856141262009867No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT10221.061542456504804No Hit
AGGGCACCGTTTGCTTAACACTGACGACATGGTTCTACAAGGGACGAACT9070.9420929628667878No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGTGAGAG9010.9358608153726306No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT7210.7488963905479097No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGT6260.650220721890418No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA6140.6377564269021033No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC6020.6252921319137886No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC5810.6034796156842379No Hit
GGAAACCGTAGCTGCCCTGCTTAATGTCTCATTCACACCACCTCAGCAAC5570.5785510257076084No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGTGAGA5290.5494676707348741No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC5100.5297325370033757No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGTGA4930.5120747857699299No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC4770.4954557257855103No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC4550.4726045183069333No Hit
CTGAAAGCTGTACCATACCTGTTTGGTCTTGGCTGAGGTTTCAATGAATG4030.4185925733575695No Hit
TCAAATGTAGCAAATCAGAAGCCAGCTGGAGTGTTCTAGAATCCTATGTG3830.39781874837704495No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG3730.38743183588678265No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG3660.3801609971435991No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA3550.3687353934043106No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA3440.3573097896650221No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGTG3330.34588418592573356No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACT3140.3261490521942353No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGTGA2810.29187224097636977No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAGTGAGA2750.2856400934822124No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGTGAG2750.2856400934822124No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT2600.270059724746819No Hit
GCTGGTACTGAGCAAGAAGACATGGTGGTTGATCTTAAGGTAACATAGAC2560.2659049597507141No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAGAGACCAAGTC2380.24720851726824203No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT2190.22747338353674368No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA2100.21812516229550769No Hit
TAGTTCATCCCGAGCAAACCTGTTCAGGAGGAGAGAACATCCCTGGTTAA1960.20358348480914046No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGTGAG1910.19839002856400934No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA1880.19527395481693066No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT1790.18592573357569464No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG1740.1807322773305635No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACGAGA1610.16722929109322254No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT1560.1620358348480914No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGTGAGAGACAATC1540.15995845235003894No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT1390.14437808361464555No Hit
ACACCACTCTCTAAATCAAACACATTGGAGACCAAGTCTCTGCTACCGTA1380.14333939236561932No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTTAATGAATG1300.1350298623734095No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT1240.12879771487925212No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC1210.12568164113217348No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAGTGAGAGA1180.12256556738509479No Hit
CTGAAAGCTGTACCATACCTGTCTAGTCTTGGCTGAGGTTTCAATGAATG1180.12256556738509479No Hit
CCATTTTCTTCACACCTGTCGTCAGTGTACCAGGTTAGTGTGTAAATCCA1110.11529472864191118No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT1080.11217865489483252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)