FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96275
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG1085111.270838743183589No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG91179.469748117372111No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA87119.048039470267463No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT81698.485068813295248No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA50395.233965203843158No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC32713.3975590755647884No Hit
TACTGTAGGGGCTCGGTGAATGGGAAGAAATAGTGGCAACTGGAGCCAAG28652.97585042846014No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT28022.910412879771488No Hit
GGCAAGGACAGGGAAGGGGCCGTGTAGAACCATTCGTGTAAAGCGGAAGG24432.5375227213710723No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT21482.2311088029083352No Hit
TCCCCTGGAAACTGCACTGTGTAGGTGCTGGACTGGTAGGCTGTAGAACC19442.019215788106985No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT18581.9298883406907295No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT15991.660867307192937No Hit
GGGATTCCATGATTCGATGTTCACGCTTACATTCACGCTTATATTCACTC15211.5798493897688912No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA13421.3939236561931965No Hit
TCACAGCCATTAAGAGGACAAACTTACGGTAGCAGAGACGTGGTCTTGAC9921.030381719034017No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA9380.9742923915866009No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC9180.9535185666060764No Hit
TCATTACACCAGTTCGTCCCTTGTAGAACCATGTCGTCAGTGTTAAGCAA8570.8901584004154766No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT6460.6709945468709426No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG6330.6574915606336017No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT6240.6481433393923656No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG5950.6180212931706051No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA5870.6097117631783953No Hit
GGTTGCTGAGGTGGTGTGAATGAGACATTAAGCAGGGCAGCTACGGTTTC5520.5733575694624773No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT5350.5556998182290315No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG5160.5359646844975331No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT4930.5120747857699299No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG4750.4933783432874578No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG4700.48818488704232665No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG4540.47156582705790706No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA3720.3863931446377564No Hit
TGTGAGCTGGTCTGAATGTTTGTCCCTCACATAGGATTCTAGAACACTCC3700.384315762139704No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT3560.3697740846533368No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA3400.3531550246689172No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT3390.35211633341989096No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG3140.3261490521942353No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT2800.29083354972734354No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC2710.2814853284861075No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT2680.27836925473902885No Hit
ATGTTACCTTAAGATCAACCACCATGTCTTCTTGCTCAGTACCAGCTGTA2570.2669436509997403No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG2550.2648662685016879No Hit
GTTGATACAAAACAAGCCCACGAACTAGCCAAGAGTTACGGGATTCCATT2410.2503245910153207No Hit
CTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA2360.24513113477018958No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC2210.22955076603479616No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC2040.2118930148013503No Hit
GCCCACTCTGGGTCCTTAGGGACAAATAATAGTTAACCAGGGATGTTCTC2000.20773824980524538No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1840.19111918982082576No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT1800.18696442482472087No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT1750.18177096857958971No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG1740.1807322773305635No Hit
CGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT1640.17034536484030122No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT1550.16099714359906517No Hit
CCAGTCCTCAGATAAATGGTGACTGAGTGTAGAACCATGTCGTCAGTGTA1530.15891976110101272No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT1530.15891976110101272No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT1410.146455466112698No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT1340.13918462736951442No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC1220.1267203323811997No Hit
CACTGTAGGGGCTCGGTGAATGGGAAGAAATAGTGGCAACTGGAGCCAAG1210.12568164113217348No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG1170.12152687613606855No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGATTGAGTACAAACTGGTGGTG1150.1194494936380161No Hit
TGGATTTACACACTAACCTGGTACACTGACGACAGGTGTGAAGAAAATGG1060.11010127239678005No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT1040.1080238898987276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)