FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114682
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT1756215.313649918906192No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC90877.923649744510907No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG70926.184056783104585No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA55404.830749376536858No Hit
GAGTCACAGTCACAGGTAGGTTGTAGCAGAAAGTCAGTCCCATGGAGACC40183.503601262621859No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC32022.7920685024676932No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT31412.7388779407404824No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA30292.6412165815036364No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC28042.445021886608186No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC27162.3682879614935213No Hit
CTGGTGTTGTTGGGCAGTGTAGAGGAGCTTGTCCCGCTAGACCAAGTCTC23952.088383530109346No Hit
GCCACAAAGTAAAAATGTTGTGTGTTTACTTTTTTGCATCTTGGCAGGCT20071.7500566784674143No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA16451.434401213791179No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT16211.4134737796689978No Hit
CACCTCGAACACCCAGGCACACTGACGACATGTTTCTTCTATAACCCACA15981.3934183219685738No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC15971.3925463455468163No Hit
ATCCTGTTCCAAGTCAAGACCACCTCCTGGACTATGTCCGGAGACCAAGT13381.1667044523116095No Hit
TGGTGATAGATGACTTGCTGCTACCGTATAATGAAGTCCAGTCTACCACA13211.151880853141731No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT13201.1510088767199733No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG12331.0751469280270662No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT12201.063811234544218No Hit
GCTTCCAGTACTTCAACTTTGCCCCCTTCTACTCACTCAAAGACATCTGC10720.9347587241240997No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG10030.8745923510228284No Hit
AGAAATGTATAACCAAGTCAGTAAGGCCAACTCAGTTTTGAGGTGTTTGA9440.8231457421391326No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGCA8280.7219964772152562No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC8170.712404736575923No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC7550.6583421984269545No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA7460.6504944106311366No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT7200.6278230236654401No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC6990.6095115188085315No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA6950.6060236131215012No Hit
GTTGTATCCGCTGCTTTGTCCTCAGAGTTCTCACAGTTCCAAGGTTAGAG6100.531905617272109No Hit
GATTCCCGCGCCCAGAGCCTGCTGTCTGTGCTTTTCATTTCATCTTCTTA5780.5040023717758673No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT5560.48481889049720095No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG5320.4638914563750196No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC5290.46127552710974695No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG4970.43337228161350516No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC4960.43250030519174765No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTAGCAAATTTTTAGATCCAG4910.42814042308295985No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA4890.42639647023944477No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA4650.4054690361172634No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTGA3950.3444306865942345No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGCACGTA3950.3444306865942345No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC3750.3269911581590834No Hit
CTTCTTCAGGTCAGGGGAAAGATGAGGCCTTTGCATCCCTAGACCAAGTC3610.3147834882544776No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC3400.29647198339756897No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC3380.29472803055405383No Hit
TCAAATCATCCATTGCTTGGTACGTAGCAGAAAGTCAGTCCCATGGAGAC3100.27031269074484227No Hit
ATGAGCAAGTTGGGGTGTGCCTGGGTGTGGGTGTGTCTGTTGTGATGTGG3060.26682478505781204No Hit
AGATTCCCACTTACCTCTGCGAAGTGCCAGGTATAAAGTTTTTCCCACTG3050.2659528086360545No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC3000.2615929265272667No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT2840.2476413037791458No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAAAGA2820.2458973509356307No Hit
GGGAAAAGAACAGGCTTCACCTATCATCGTTTTCCAAGTTATTTTTGAGA2780.24240944524860047No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGCAC2560.22322596396993424No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGCA2530.22061003470466156No Hit
GCCTACCAAAATCAGAGAAGCCATCTCAGCTCATAAAAACAACTGCTTGC2510.21886608186114648No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG2490.21712212901763137No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC2430.21189027048708603No Hit
TCTCCATATCTCTCTCAATTTCAACAAGACAAACAACAGTTGGTATTAGG2360.20578643553478315No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGCACGT2280.19881062416072268No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGCAC2280.19881062416072268No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGC2240.19532271847369245No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT2220.19357876563017737No Hit
TTCAGAAGACAATGTTGATGTTCATGATCATGGTTCTACAGTCCCACAGA1930.16829144939920826No Hit
GGATGCAGAGCTTCTTCCCACTTTGTGTAGAACCATGACTTCAGAGAGGG1860.16218761444690535No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT1860.16218761444690535No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG1740.15172389738581468No Hit
CTGGTGTTGTTGGGCAGTGTAGAGGAGCTTGTCCCGCGAGACCAAGTCTC1670.1456200624335118No Hit
TATTTAGTAGCCAGGACAGTAGAAGGACTGAAGAGTGAGAGGAGCTCCCA1540.1342843689506636No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC1480.12905251042011825No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT1400.12207669904605781No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT1350.11771681693727003No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGCACG1310.11422891125023979No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG1240.10812507629793691No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG1230.10725309987617934No Hit
AGATTCCCACTTACCTCTGCGAACACTGACACAATGGCAACAGTTCTGAG1220.10638112345442179No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGCACG1180.10289321776739158No Hit
GTACAATTTCTACCGCTGGTTCCTCTGGTCTCTTCTGAAGCCTGCAGTGA1170.102021241345634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)