FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114682
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT1562713.626375542805322No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG91677.993407858251513No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT71046.194520500165676No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT54004.708672677490801No Hit
CCATGGGACTGACTTTCTGCTACAACCTACCTGTGACTGTGACTCTGTAG40253.5097050975741615No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC31272.7266702708358763No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT30522.66127203920406No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA30252.637728675816606No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC27912.4336861931253377No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA24772.1598855966934654No Hit
AGCGGGACAAGCTCCTCTACACTGCCCAACAACACCAGTGTAGAACCATG23952.088383530109346No Hit
TCAAAAGGAGATACTTACACAACAGGAAAGACTTTGGGAAACACAACACT18301.595716851816327No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT15701.3690029821593623No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTGCCTGGGTGTTC14871.2966289391534853No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC14791.2896531277794248No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA14481.2626218587049405No Hit
CCGGACATAGTCCAGGAGGTGGTCTTGACTTGGAACAGGATTGTAGAACC13211.151880853141731No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT13111.1431610889241555No Hit
GGTGTGGTAGACTGGACTTCATTATACGGTAGCAGCAAGTCATCTATCAC13021.1353133011283374No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG12471.0873545979316719No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA12461.0864826215099144No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG11631.0141085785040371No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC9910.8641286339617378No Hit
TCTCCAGGTAATAAGGGTGGGAGGGGCAAAGGAGGCAACGTTGAAGTTTT9610.8379693413090109No Hit
TCATGCTTATCAAACACCTCAAAACTGAGTTGGCCTTACTGACTTGGTTA8680.7568755340855583No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA8260.7202525243717409No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG7430.6478784813658638No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT7370.6426466228353185No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC7170.6252070944001674No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA6740.5877121082645925No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT6650.5798643204687746No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA6610.5763764147817443No Hit
ACATGTTAGCTGACTGATGATGGTCAATTTATTTTGTCCATGGTGTCAAG5620.49005074902774626No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT5590.48743481976247366No Hit
TCCCCTGGAAACTGCACTGTCCTTCTTCCCTAAGAAGATGAAATGAAAAG5460.4760991262796254No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC5290.46127552710974695No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT4920.42901239950471737No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA4870.4246525173959296No Hit
GCTGAAAGTCTGGATCTAAAAATTTGCTACGGTAGCAGATACATTGGACC4850.4229085645524145No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA4780.4168047296001116No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC4770.4159327531783541No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA4630.40372508327374834No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC4000.3487905687030223No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT3940.3435587101724769No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG3770.3287351110025985No Hit
AGGGATGCAAAGGCCTCATCTTTCCCCTGACCTGAAGAAGTGTAGAACCA3560.31042360614568987No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG3390.29560000697581135No Hit
CCATGGGACTGACTTTCTGCTACGTACCAAGCAATGGATGATTTGATGTA3150.27467257285363006No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG2960.2581050208402365No Hit
CAGAACTGTTGCCATTGTGTCAGAATCCTTCAGGTTCCTGTTCCGTAGAT2940.25636106799672137No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT2830.24676932735738827No Hit
ACAAGTCAACAACCCCCACATCACAACAGACACACCCACACCCAGGCACA2820.2458973509356307No Hit
CAAAAATAACTTGGAAAACGATGATAGGTGAAGCCTGTTCTTTTCCCTGT2770.24153746882684293No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT2770.24153746882684293No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT2640.23020177534399472No Hit
CAGGACTAGCTCTAAGAAACCTGTGCCTTGCAAGCAGTTGTTTTTATGAG2590.22584189323520693No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA2590.22584189323520693No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA2500.2179941054393889No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG2450.21363422333060114No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT2300.2005545770042378No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT2280.19881062416072268No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG2240.19532271847369245No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT2180.19009085994314714No Hit
CTGAGTAAGTGGCAAGAAAATTACCTGCGTGCTGCATCAAAGTTGCTTTT2180.19009085994314714No Hit
AGAAAAAGTTTCAGTTTTACCAATTTCCATTTTTACGTTTTTAGGTGAAG1920.1674194729774507No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT1890.164803543712178No Hit
CCATCTGGACCCTCTCTGAAGTCATGGTTCTACACAAAGTGGGAAGAAGC1850.1613156380251478No Hit
CAGCCATATCAGTCTGTGGGACTGTAGAACCATGATCATGAACATCAACA1790.15608377949460245No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT1700.14823599169878449No Hit
CGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA1680.14649203885526935No Hit
CGCGGGACAAGCTCCTCTACACTGCCCAACAACACCAGTGTAGAACCATG1660.14474808601175423No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT1460.12730855757660312No Hit
GCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT1420.1238206518895729No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC1410.12294867546781535No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA1390.12120472262430024No Hit
CAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT1360.11858879335902757No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG1360.11858879335902757No Hit
CCAAAAGGAGATACTTACACAACAGGAAAGACTTTGGGAAACACAACACT1330.1159728640937549No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC1290.11248495840672469No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT1250.10899705271969445No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCC1250.10899705271969445No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG1220.10638112345442179No Hit
CAGAACTGTTGCCATTGTGTCAGTGTTCGCAGAGGTAAGTGGGAATCTTG1200.10463717061090667No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1200.10463717061090667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)