FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712157
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT154132.1642699573268254No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG148032.0786146874916627No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT134791.8927006264068176No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT117491.6497766644152905No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG116291.6329264473985372No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA99281.3940746211860586No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC93631.3147381827321785No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC92821.3033642862458699No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA91181.2803356563229737No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG90761.27443808036711No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG90291.2678384120355484No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG86101.2090030709520514No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT84221.1826043976258045No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA80431.1293857955478919No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC77221.0843114650280765No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA77031.081643514000424No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA76631.0760267749948396No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG71851.0089067438781054No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA69300.9731000327175048No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG67080.9419271312365111No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT62470.8771942141971504No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT62330.8752283555451958No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT59860.8405449921857119No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT58730.8246777044949358No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT57780.8113379493566728No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA57000.8003853082957831No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC56750.7968748464172929No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA55950.785641368406124No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA55350.7772162598977472No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT53990.7581193472787602No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG52300.7343886249801659No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC51150.7182405003391106No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA49760.6987223322947047No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC49590.6963352182173312No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG48700.6838379739299059No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA48200.6768170501729254No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG48070.6749916099961104No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC47600.6683919416645487No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC46470.6525246539737726No Hit
ACCTGTATAGGGTATGCTCTTTGAATAATTACATCAACACAACCAACATT46280.64985670294612No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG44280.6217730079181978No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA44040.6184029645148472No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA43360.6088545082053536No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA43300.6080119973545159No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT43270.6075907419290971No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT42620.5984635410450223No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA41650.58484294895648No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG41620.5844216935310613No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC41030.5761370034978243No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT40330.5663077102380515No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC40030.5620951559838631No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT39630.5564784169782786No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC38000.533590205530522No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG37290.5236204937956097No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT36770.5163187330883499No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT36760.5161783146132103No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTTGGTGCCATTTATCGTTTTTG35700.5012939562484114No Hit
GTCACTGACAACTGGCTTGTGCAACATTTTGACATGGAAGTCACAGACTA35680.5010131192981323No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA35660.500732282347853No Hit
AGGGCCTATTAGCCATATCTCCCAGCTCTCAACAGGAGGTGGAGAGGCAG34380.4827587175299829No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA33360.46843603306574255No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG33100.4647851527121126No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC31780.44624991399368397No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT31730.44554782161798595No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA31290.43936940871184305No Hit
CCTCAGCTTGCCATCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC30970.43487601750737553No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA29960.4206937515182748No Hit
TGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCT29850.41914914829173905No Hit
CACTGGGCAATTGGTAGGGTAAATCCACAGGGCGCCCGCGGAGGTGCAGA29820.4187278928663202No Hit
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG28750.40370311602638187No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA28130.394997170567726No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA26620.37379398082164467No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA26540.3726706330205278No Hit
TATGAGAACACGCAGAGGGAACTTGGCCTCCTACTGCTGCTTTCAGAGAG26010.3652284538381284No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC25610.35961171483254395No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC25450.35736501923031017No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT25440.35722460075517054No Hit
ATGTTACCTTAAGATCAACCACCATGGACTGAACAAGTAGGAAAATGACA24530.34444651951746597No Hit
TTATCGCCATCCTTCTGAACTTAAGAAGAGCTCTAAAAAGCCAGTGTACA24320.3414977315395341No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG23660.3322301121803198No Hit
GGTGAAATAAAGGAAGATACTAGTTTTGGGTCTAGTGAGGATGAAGAGCT23130.3247879329979204No Hit
CAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCA22770.3197328678928944No Hit
GTGTCAATGGCACTGAACCCTGTTTCAGGCCCATCAGTGTTTCCATAAGT22610.3174861722906606No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT21750.3054101834286541No Hit
GACAGGTTCATGAGTGCTTCCCCCACATTCCTTCAGGTGACTGGTTCTTC21620.30358474325183915No Hit
AAGTACATATATTTAGCAGATCAGTTAACAGACAAAAGTCAACTTTACAG21530.30232097697558263No Hit
CTGAGTAAGTGGCAAGAAAATTACCTGCGTGCTGCATCAAAGTTGCTTTT20670.2902449881135761No Hit
AATGCAGATGATTACCTCGCTCTGGCAAATGATTTCATTAAAGATTCAGA19970.2804156948538033No Hit
TTCTTCACTTGATTGGCCTTTGCTGGATTCCTTATCTTATAGTTTTGTAG19950.28013485790352405No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA19840.27859025467698834No Hit
ATTAGGATGTCTGGCACATGCACAGGACAACCAAGTTCAAGAACCTCTCA19130.268620542942076No Hit
AGAAAAAGTTTCAGTTTTACCAATTTCCATTTTTACGTTTTTAGGTGAAG19070.2677780320912383No Hit
GTGTCCTGCTTATTTTTCTCACATTCTTCCGTACTGGCCTGGGAACTCTC18830.2644079886878876No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT18270.25654455408006943No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG18270.25654455408006943No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC18050.25345534762699795No Hit
AAAAGCCCCTAAACCCCACTTCATTTTCATCTGTTAAATAACCAGAAGCA17780.24966404879822848No Hit
CACGCACACACAAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACG17760.2493832118479493No Hit
ACACCGGATATCTGCTTCCTCACAGAGGTGGCGGAAACAGGACATGGCAA17430.2447494021683421No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC17430.2447494021683421No Hit
GCTGACCTAAAGCCACCTCCACCTCTCTCCCCCTAGAGCAGGTGCCTGCA17410.24446856521806287No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC17350.24362605436722523No Hit
TCAGATTGAGACCTCCTTTCCACCACCATGCGATTACTCACGATGTAATC17140.24067726638929338No Hit
TGGATTTACACACTAACCTGGTAGAAATGCGACTAAAGACTGCATTGAAG17100.24011559248873493No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTAGGCTGCAGCGACTTCGAACT17080.23983475553845574No Hit
CTGAGGTCGCTCAGTTCCGGAGCTGCAACCCGGAGTCCCAGATGATCGGG16820.23618387518482584No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA16400.23028629922896218No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC16330.22930336990298486No Hit
CGACTCAGAGACCGCTTGCTCAGGAAAGGGCTGCTGGACCGCCTTCGACC16170.22705667430075108No Hit
AGAAAGAAATATATGGTAAGTTTCAAGAATACATCATACCTGTATAGGGT15930.22368663089740046No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA15870.22284412004656273No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG15670.22003575054377056No Hit
TTCTTCACTTGATTGGCCTTTGCATGGTCCTACAGGTTCCTTTTCCTTCC15500.21764863646639715No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG15120.21231273441109194No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA15090.21189147898567312No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAGTGCAAACAATACATCATACTT14850.20852143558232245No Hit
TATGAAGTAGCCTGCTATAATCGATCAAACTAACCCTAACTATCAAGATG14820.20810018015690357No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA14480.20332595200215683No Hit
GTTCTGGAGTACGTATAGCAGTATTTTCTTCTCTTGTGAGCTGGTCTGAA14460.2030451150518776No Hit
CTGTGTCACCAAGGGTTTCGTTGGGGCCATGAAGACCACCTTTCCTGAAG14300.20079841944964386No Hit
CGAGATGTTCATTTTCCCCAAACTCACAAAGAGAATTCTTCTCGTAGACG14030.1970071206208743No Hit
AGAACATTTCCTCAGAATTGTCCCAAAAGAGCTAGTTAAGGACAAAGTTG13850.19447958806836133No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT13440.18872243058763727No Hit
ACCAGAATCTAAAACATTTCCAGCAAAGTGGCACATATCCCTAGCTCTCA13420.18844159363735807No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT13200.1853523871842866No Hit
TCAAAAGGAGATACTTACACAACAGGAAAGACTTTGGGAAACACAACACT12910.18128025140523787No Hit
TGCCTGAATGTTCTTCACCACCTTCATGCAAACGTAAAGTTGGTGGTACA12790.17959522970356256No Hit
ATTGGTGATGATTCGATGGAGTATTGGTAAGACAGCTAAGAGGGGAGGAT12720.17861230037758527No Hit
AATTACCTCCAGTTGCTCCTTCTGGGTAAAACCCTTCGTGCTTTTCCCTG12650.177629371051608No Hit
ATTACTAAGTCATAAAAATAAACCAGGTAGAATATTTACCTTCACAAACT12560.17636560477535151No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAACAGAAACCTTCATTT11870.16667672999071834No Hit
TCTGAGCACCAGTGGAGATTTTGTTAAGCAAACGGTGCCCTCAGAAAATA11870.16667672999071834No Hit
GTCTCTGTATGGCAGCAAATGTGGCCCATGGCCTGCAGCAAAACGTCCTA11530.16190250183597157No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT11480.1612004094602735No Hit
CATTCCTTGTTGTGCTCAGTACTGACTTTGGTATGTGTGTTCTGCTGTCC11140.15642618130552674No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCTGGAAAATAAACATGAC10990.15431990417843255No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT10860.15249446400161762No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC10830.1520732085761988No Hit
TCCTTCTTTTTCCTACTGAGTTCAGCTCCCAGACTGGAATTAAGTGGAAG10640.1494052575485462No Hit
ATTGGACCAGTCTACCCTGTAAAAATGTGATTCTTTGCTAGTACCTTTGT10610.14898400212312735No Hit
CCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGCAAGTGGCTCCTTG10450.14673730652089356No Hit
CATATTAGAAATAACAATGTGTACCATATAACTAATTTTACCTTCATGTT10450.14673730652089356No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCAAAACATGAATGTTCTCAAC10300.1446310293937994No Hit
GCAATGGTTAAGGTGAACTGGTTACACTGACGGGGTGAAGTGGAGATGTA10250.14392893701810136No Hit
GGGCTGTAGAGAATGATTGATGTACACTGACGTCATTTCAACTATCCAAG10230.14364810006782214No Hit
ACTTTGTAATTCAACATTCATCGTTGTGTAAATTAAACTTCTCCCATTCC10150.14252475226670525No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT10040.1409801490401695No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACATCCC9870.13859303496279612No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTAGAACACAGAGGA9860.13845261648765653No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG9270.13016792645441946No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA9250.12988708950414024No Hit
CACTCCACCCCCAAGAATATCATCTAGTGCTGAACAGAAGGTGATTATGA9120.1280616493273253No Hit
TGCACGTACCACATTTAATTACCTTGTCTTCTCTTGGTATTAGTGCTTGA9050.127078720001348No Hit
GTTATCAGCTTGTCAAGAATAGCAGTAACTCTTTGTCGTTTGGCATCATC8890.12483202439911424No Hit
GCAGGTGACCAACCACATAACTCGAGACAGGGACAGCGGGAGGCTCAAAC8750.1228661657471597No Hit
ACTAAAATGTTTATCTGATTAATTACCCACCTAATGCAGGAACAAGCAAG8090.11359854638794535No Hit
TCCTTGGATGATGATGCTTTCACGGCTCCATTTCATAGGGATGGAATGCT8020.11261561706196808No Hit
TCTCCAGGTAATAAGGGTGGGAGGGGCAAAGGAGGCAACGTTGAAGTTTT7910.11107101383543236No Hit
CCAGAAGTGATTTCATGACCACCTACAAGAATACAGGTTCCTTTTCCTTC7810.10966682908403624No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT7780.10924557365861742No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCC7600.10671804110610442No Hit
AGTTGAAGAAGCACCCTTTCTGGGCTTAGGCATCTATTAGCAAATTCCTT7590.10657762263096479No Hit
GCAATGGTTAAGGTGAACTGGTTCTCATCTACTAGGCAGATTTCTTCAAT7530.10573511178012714No Hit
GTCACCAACCCCATGTCTTTGGAGTGTTTGGACTTATGCCGCTTGTGCTT7470.10489260092928947No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG7340.10306716075247453No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTATTTTCTCCATCTGGGCTC7300.10250548685191607No Hit
ACAGAGAGTGAATGATATTAATGACCAGTCCATGTGTGGAAGCTCTAAGG7290.10236506837677647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)