FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71001
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC982913.843466993422629No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC61348.639314939226209No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC55867.867494824016563No Hit
AGATGATACTGCTGCCCTCGGTCACAGTGCAGTTTCCAGGGGAAGACCAA35034.93373332769961No Hit
GCCGGAACAGCCCCAGCCCTTCCCTGTCCTTGCCAGACCAAGTCTCTGCT34374.840776890466332No Hit
TTTCTTTGCTGGATCTGGAGGTGGTGTGTGATTGTGGAAAAGGACAGAGA27573.88304390079013No Hit
TCTATGCTTGTTTCCCGACTGTGTGTGGCAGAGCAAAGGGCCCCAGACTG26913.7900874635568513No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC25213.550654216137801No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA14692.06898494387403No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA14071.981662230109435No Hit
TCTCCACTTCACCCCGTCAGTGTAACCAGTTCACCTTAACCATTGCAGAC13011.8323685581893214No Hit
CTCCTTTCTGGACGCTTTTGCTACCGTATGGAGAGAAATCTGTATTAACA10431.468993394459233No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC8711.2267432853058406No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA7881.1098435233306574No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG7851.1056182307291447No Hit
GAGATTGATGACCAAAAGAACTGCTACACTGACGACATGGTTCTCCTCAG7141.0056196391600118No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT6920.9746341600822525No Hit
TACTCTTCTTGGCTCCAGTTGCCAGGGCAGAATACAGAGTATCATTCCTT6910.9732257292150815No Hit
GTCCTCCAGAGCACAAACCTTCCCACAGACTGATATGGCTGAGACCAAGT6340.8929451697863411No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTACCTTCAGAGCTAGGGAAAAA6200.8732271376459486No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGAG5810.8182983338262841No Hit
TCTCCCCCACCCCCTCTACTCCCAGGTCACCGTTTTGATGCCGACGAGTA4980.7013985718511007No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA4890.6887226940465627No Hit
TCTTGCTTTTTTATTTCAGGATGCTTGCCACCGGACCAGGAGCCCACTTC4560.6422444754299236No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGAGATCA4320.6084421346178223No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT4110.5788650864072338No Hit
GGAAACCGTAGCTGCCCTGTTGATACGGAAGTGCTGGGAAGGAAAGAGCA4100.5774566555400629No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA3960.5577386233996704No Hit
TTCAGGTGTATTTTGGAGGCCATCTCAAGTAACAGGCAGGTCCTTCAGAC3600.5070351121815185No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGA3540.4985845269784932No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA3470.4887255109082971No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT3350.4718243405022465No Hit
TCGGGGGCCTACAAGAGTCTGACAGAGTTCTCATTACCCAAGACCAAGTC3150.44365572315882873No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGAGATC2700.3802763341361389No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA2660.3746426106674554No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC2650.3732341798002845No Hit
CACCTCGAACACCCAGGCTGGAGTGCAGCAGCATCTTGAATCTCATACAG2640.37182574893311365No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC2620.36900888719877184No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC2500.35210771679272124No Hit
TAAAGGAACTGTAGTCGCCCTGGTGGTGGTAATTTTAAAGCCCTAGACCA2430.34224870072252506No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGAGA2360.3323896846523288No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGAGA2350.33098125378515797No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT2310.3253475303164744No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAGAGACCAAGTC2270.3197138068477909No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGAG2230.31408008337910737No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG2030.2859114660356896No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAGAGATC1940.2732355882311517No Hit
AGATGATACTGCTGCCCTCGGTCACAGTGCAGTTTCCAGGGGGAGACCAA1750.24647540175490484No Hit
CCAAAATATGTCTGGATTGGAGAATACGGTAGCACAGCAAGTGGAAAATC1620.2281658004816834No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG1590.22394050788017072No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGAGAT1460.2056309066069492No Hit
GTTCAGCCCAGTTTGAAGCATACGGTAGCAGATGACGATTCCTTCTGTGT1350.19013816706806946No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGAGAT1330.1873213053337277No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT1210.17042013492767707No Hit
TCTTAAATGGCTACGACCCAGCCCTGTCTCCAGGGGCCGAGAACTCGCCA1170.16478641145899353No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT1150.16196954972465177No Hit
TGTAAATTTCTTTGATCAGAAACCAGAAGCCCAAAATCAAGGTGTTGGCA820.11549133110801257No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG790.11126603850649991No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)