FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71001
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG986613.895578935507952No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA60878.573118688469176No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA55697.843551499274658No Hit
TCCCCTGGAAACTGCACTGTGACCGAGGGCAGCAGTATCATCTTGTAGAA35234.961901945043028No Hit
GGCAAGGACAGGGAAGGGCTGGGGCTGTTCCGGCTGTAGAACCATGTCGT34544.864720215208237No Hit
CTGTCCTTTTCCACAATCACACACCACCTCCAGATCCAGCAAAGAAATGT27603.8872691933916426No Hit
GCAGTCTGGGGCCCTTTGCTCTGCCACACACAGTCGGGAAACAAGCATAG26863.783045309220997No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG25203.54924578527063No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT14652.0633512204053464No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT14041.9774369375079224No Hit
GCAATGGTTAAGGTGAACTGGTTACACTGACGGGGTGAAGTGGAGATGTA13031.8351854199236632No Hit
CAGACTGTTAATACAGATTTCTCTCCATACGGTAGCAAAAGCGTCCAGAA10321.4535006549203533No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG8071.1366037098069042No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT7891.111251954197828No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT7781.0957592146589483No Hit
TTAAATGGAGTCATCTGAGGAGAACCATGTCGTCAGTGTAGCAGTTCTTT6930.9760425909494232No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCCTAATCCAT6800.9577329896762018No Hit
TACTGTAGGGGCTCGGTGAAGGAATGATACTCTGTATTCTGCCCTGGCAA6500.9154800636610751No Hit
CAGCCATATCAGTCTGTGGGAAGGTTTGTGCTCTGGAGGACTGTAGAACC6340.8929451697863411No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA5870.8267489190293095No Hit
ACTTTTTCCCTAGCTCTGAAGGTACGGTAGCAGACCTTTGTGCTTTTATC5430.7647779608737905No Hit
ATGAGGTACTCGTCGGCATCAAAACGGTGACCTGGGAGTAGAGGGGGTGG4990.7028070027182716No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG4940.6957648483824171No Hit
CCAGTACTAATGAAGTGGGCTCCTGGTCCGGTGGCAAGCATCCTGAAATA4560.6422444754299236No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC4330.6098505654849932No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT4160.5859072407430882No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT3880.5464711764623034No Hit
ACACTTTGAATGCTCTTTCCTTCCCAGCACTTCCGTATCAACAGGGCAGC3720.5239362825875692No Hit
GAAGGACCTGCCTGTTACTTGAGATGGCCTCCAAAATACACCTGAATGTA3640.5126688356502022No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG3600.5070351121815185No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT3420.4816833565724426No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC3340.47041590963507557No Hit
TGGGTAATGAGAACTCTGTCAGACTCTTGTAGGCCCCCGATGTAGAACCA3130.4408388614244869No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT2720.3830931958704807No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG2700.3802763341361389No Hit
TATGGTCCTCAGAAAAGTGCACCAGATCATCTTCCATTTGTAATACTTCT2640.37182574893311365No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG2630.37041731806594275No Hit
GTATGAGATTCAAGATGCTGCTGCACTCCAGCCTGGGTGTTCGAGGTGTG2600.3661920254644301No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG2490.35069928592555033No Hit
AGGGCTTTAAAATTACCACCACCAGGGCGACTACAGTTCCTTTATGTAGA2410.33943183898818324No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT2380.3352065463866706No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT2330.32816439205081616No Hit
CTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA2290.32253066858213264No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA2270.3197138068477909No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT2250.3168969451134491No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA2080.2929536203715441No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT1960.27605244996549344No Hit
CCCCCTGGAAACTGCACTGTGACCGAGGGCAGCAGTATCATCTTGTAGAA1750.24647540175490484No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT1540.21689835354431627No Hit
ACAGATTTTCCACTTGCTGTGCTACCGTATTCTCCAATCCAGACATATTT1490.20985619920846185No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC1420.19999718313826564No Hit
AAACACAGAAGGAATCGTCATCTGCTACCGTATGCTTCAAACTGGGCTGA1320.18591287446655683No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1300.18309601273221504No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT1190.1676032731933353No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG1170.16478641145899353No Hit
GGATCCGTGGCGAGTTCTCGGCCCCTGGAGACAGGGCTGGGTCGTAGCCA1130.15915268799031002No Hit
CTACCAGTGCCAGGAGCCAGTCCTGCCAACACCTTGATTTTGGGCTTCTG820.11549133110801257No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT750.10563231503781638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)