FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262611
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA115164.385193308734212No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC87033.314027211350629No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT77922.9671262818389175No Hit
TGAACTTATATATTTGACACAACCCAGCAGTTCGTAAGTAGTTCACAGAA73452.79691254364821No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC68782.6190829782453897No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA67802.5817654249060396No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC61892.356717730788124No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA61622.3464363640517725No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT60682.310641976154845No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT56612.1556598923883614No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG54412.0718857930551273No Hit
TGGTGATTTGCTGATATACCATGTCTCTGCTACCGTAGCAAATGTGTATT53802.048657520058185No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG51651.9667873775279787No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA47881.8232290345796636No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC41241.5703835711375382No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG39781.514788032489119No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGGGCAAAATGAGATATTGTGATAGA36051.372752854983226No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT35101.336577675725693No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA32771.247853288704586No Hit
GAATCAGGGATCACAACACCCCCCAAAATGAGGAGAGTAGCAGAAACTGA32031.2196747280197706No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT31731.2082509872016023No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT28621.0898248740532575No Hit
TCTCCACTTCACCCCGTCACCACCACTTTCCAGGTTGGTTCTACTGCTGT25230.9607366028079555No Hit
GGGAAGCTCTTCATCCTCACTAGACCCAAAACTAGTATCTTCCTTTATTT24350.9272269630746618No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC24320.9260845889928448No Hit
TTCTGATGAGCCAAGTCTCTGTGACTGTGATGTACATAAACATAATCAAA23870.9089489777655925No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGGTACATGT23270.8861014961292558No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT22880.871250633065637No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC22820.8689658849020034No Hit
TTATGGAACAGACTTTATAGATTAAGGGACACACTGACGACATCGTTCTA22130.8426912810202162No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA21510.8190822166626683No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATAG20770.7909036559778532No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC20490.780241497880896No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA20080.764629052096066No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA20010.7619635125718268No Hit
AAGGGAAAAGGATGGATTAGGGTAGAGATTTTATTTTGTTTTTCAGAGAC19400.7387352395748845No Hit
ATTCATGGGTTGCAAGTAGAAGTTTGTCCTCTTAATGGCTGTGATTACAT18620.7090335134476469No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT18300.6968481899082674No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG18180.692278693581No Hit
CTTCTTCAGGTCAGGGGAAAGGGAATGCCCAATTCAGAGAGACATGGGGG18170.6918979022203944No Hit
TGCAGGGCTGATTGTCTTCTTTTAAGGTAAAATATATGGAGCAGGTATAA17420.6633385501749737No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA17190.6545803488810445No Hit
GCTTCCAGTACTTCAACTTTGCCCCCTTCTACTCACTCAAAGACATCTGC17110.6515340179961997No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTAAGGTAATTAAAT16620.6328752413265247No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG15740.599365601593231No Hit
TTTGCAGACCTTCTCAGGAGTCACTACTTCATTCCTGCTCTTAGTGTCTG15630.5951768966265693No Hit
CCAATTTTGGTAAGCTGCCCATCTACATTATCAGGCAAATGGCTGCAAAG14220.5414853147811782No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT13810.5258728689963482No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC13450.5121643800145462No Hit
ACTTAATGGGACTGCTAGAATCTGGTTTGGTGCACTTTTCTGAGGACCAT13380.5094988404903069No Hit
GGTAGGATGTGATATTCCTTCTAGTGGAAATACCAGTCAAATGTCCATGG13320.5072140923266734No Hit
CAACTTGTATTCAGCAGGTACCGTAGCCTTCTGTACTATAGCATATCTGT13270.5053101355236452No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC12930.4923632292630545No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATA12800.48741294157518156No Hit
GGCTTCCACTTAATTCCAGTCTGGGTGCGGAAGAGAAAGACCAAGTCTCT12720.48436661069033665No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATAGTCTG12530.47713157483883006No Hit
ATATTTAGTGAATGTGATTGATGGTACTTTAATTTTGTCACTTTGTGTTT12150.46266150313581683No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC12050.45885358952976074No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA11660.444002726466142No Hit
CTCTCTAGGGGGTCTGTCTTCTGGGCATTGATGGCAAATCATTAATGTAT11380.43334056836918483No Hit
AGCAAGCTGTGACGGGTAAGCTTTATAAGGAAAGTAAATCTTCACCCCAC11370.43295977700857924No Hit
GCAACAGCTTCATTGCTGAGGGACTAGACATCACTCGCCTGTAACTTGAA10990.41848970530556606No Hit
TGCCTTGTTTCTTGCTTTCGTATAAGCCCTCACAACAACCAACACTTTAA10900.41506258306011556No Hit
GACAGCAGAACACACATACCAAAGTCAGTACTGAGCACAACAAGGAATGT10690.4070659644873977No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATAGT10590.4032580508813416No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATAGTCT9510.36213258393593567No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT9410.3583246703298795No Hit
CTACCACTGGCCACTGTAACAGTGGACGAACTCGCCACGGATCCGCAAGC8720.33205006644809243No Hit
AGATGAGTCATATTTGTGGGTTTTCATACAGCTTCCCTCTTTAAGATGTC8660.32976531828445876No Hit
TCCTGGCATGTGTTTCTACAGAGCTGATTTTCTTTAAAAGTGAATGACTC8470.3225302824329522No Hit
CGGCCCCTTCCGCTTTACGTCTGACGTCACGCCGCACGCCCCAGCGAGGG8470.3225302824329522No Hit
TCAGCTCACTTCAAGAAATCTCCATTACGGTAGCAGAAACTACCTGCTGC8190.31186812433599503No Hit
TGGTGATTTGCTGATATACCATGTCTCAGTGTAGTGGAACCAAATGATAC7880.30006359215722117No Hit
CACCCTTGAACAGTAAAAGCGAATCTTTACCTGTGTCAGACAAAACTGCT7810.29739805263298186No Hit
GCACAAGCGGCATAAGTCCAAACACTCCAAAGACATGGGGTTGGTGACCC7630.29054380814208086No Hit
GTTACTCTGTTTGATTCTCGGCATTTACTCTACCAACTGCTCTGGAACAT7510.28597431181481353No Hit
TGAATTCTCCTCAGATGACTCCATTACACTGACGACATGGTTCTACAGGG7400.28178560684815185No Hit
ATTTGTTGCAGGTTTTGGTTTTAATGGCTTGTGGCGGTTTGCAGGACCGT6770.25779575112999836No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC6640.2528454634421254No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT6340.2414217226239571No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATAG6270.23875618309971786No Hit
GGCATCTGTATATTTATTTTAAACACTGCTAATAATCTTTGTCTTTTTTG6080.2315211472482112No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG6050.2303787731663944No Hit
GTCTCAGAACAAACCTGAGATGCATGACTACTTCCCATAGGCTGTTCTAA6010.22885560772397195No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA5940.2261900681997327No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA5900.22466690275731024No Hit
ACTCTACCCTGCGATTTTCTCTCATACAAAGATCTGAATCTCTAACTCTC5870.22352452867549338No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC5850.22276294595428217No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGATATGCAGAATCCAAATTA5780.2200974064300429No Hit
AAACCTAGAGCCTCCTTTGAGCTGTGGTGTACATTGACACAGACCAAGTC5640.21476632738156434No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT5420.20638891744824092No Hit
GCACAAAAAGAAACACCCAAAAGATATATCTGGAAAACTTTCTTTCAGTG5330.20296179520279045No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT5310.2022002124815792No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATAGTC5250.19991546431794555No Hit
GCATTTTCTTCACACCTGTCGTACACTGACGACATGGTTCTACAGGGAAG4600.17516402587858085No Hit
GTAACCAGGCATTATGATCTTTGAAAATTTTTATTTGCGTATCTTTAATT4350.1656442418634406No Hit
CCACAGAGATAGCACCTAGAAAGCTGAATTAAGCAAGGAGGGGTAGAACA4320.16450186778162376No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG4260.1622171196179901No Hit
TTTGAGTTGATGCACTAATAACAGGTACTTCTCTTGCCTCCTCTTTACCA4210.16031316281496205No Hit
CCATTTTCTTCACACCTGTCGTGAAGGAAACCAGCATGCAGCTGAACTTC3960.15079337879982177No Hit
TGACACACTTGCAGAAACAGTATTGGGCAGATAGAGCTTCGTTCAGACCA3890.1481278392755825No Hit
TGTCCAGTCTATCATTCATATCCGGACCCGCTGGGAACTGTCACAGCCCG3750.14279676022710397No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT3690.1405120120634703No Hit
ACTTTCCTCTGGCAATTGGACATGCTTCGTGTTGTTCTAACATAATATTC3630.13822726389983664No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGAAAATAATCACTCTATTAAAGTTT3610.13746568117862543No Hit
ATCCATGAAGTCCAGAAGTAGAAGTCCTGCATATTCAAGACATTCATCTT3610.13746568117862543No Hit
TTCCCAATCACAGCCATTCGTGAAATCAAAATCCTTCGTCAGTTAATCCA3540.13480014165438614No Hit
GCTACTGTGTGAACCTCATCAACCATCTCATGATTATCTTTAATAGAGTC3390.12908827124530198No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATAGT3190.12147244403318978No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATAGTCTGTCAATC3050.11614136498471124No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT3040.1157605736241056No Hit
GAGGTAGCTTCAGAACAGCTTCAAATAAGGAAATCAAGCTCTCTGAACAT2780.10585999824835975No Hit
CCTCTCTCCTTCTTCCCCAAGTACACTGACGACAGGGGCCAGGAGGAGAC2690.10243287600290925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)