FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262611
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG112194.272098274634345No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG86463.292322103796109No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT72462.7592141989482544No Hit
TATGAAGTAGCCTGCTATAATCGATCAAACTAACCCTAACTATCAAGATG71782.733320386427073No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA68752.617940604163573No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC66422.5292162171424657No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT61452.3399629109214772No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG60402.299979818057888No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC56262.142332194767165No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC54442.0730281671369437No Hit
GCTCTTTCTTGTAAATACACATTTGCTACGGTAGCAGAGACATGGTATAT53292.0292371606672988No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG52772.009436009915807No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG51941.9778303269855413No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG46611.7748685317827508No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT39321.497271629901261No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG38161.4530998320710098No Hit
ATCACAATATCTCATTTTGCCCTCTTTCTTCACAGGTTTTGAAGGAATGT36061.3731336463438317No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT32611.2417606269348962No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT31931.2158668144137146No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCTGGAAAATAAACATGAC31441.1972080377440397No Hit
CTTGGGCCTGTGTTATCTCCTAGATTGGCTCTGACTGTACCACCATCCAC30631.1663639375349852No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA27881.0616463133684422No Hit
GCAATGGTTAAGGTGAACTGGTTCTCATCTACTAGGCAGATTTCTTCAAT25170.9584518546443219No Hit
GGTGAAATAAAGGAAGATACTAGTTTTGGGTCTAGTGAGGATGAAGAGCT24140.9192303445019439No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC23360.8895286183747063No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC22770.8670619280989753No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT22620.8613500576898911No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC21780.8293635833990198No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT21380.8141319289747954No Hit
AGCAAACTTTCATCTTGATCAGTGGACAATACTGGGAGGTCACTGTTATC21120.8042313535990495No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA21040.8011850227142048No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA20370.7756720015536287No Hit
AGTGACGTGTCCTCTGTTCCATAATGTAGAACGATGTCGTCAGTGTGTCC20360.7752912101930232No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT20030.762725095293038No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA19810.7543476853597145No Hit
CTGAAAAACAAAATAAAATCTCTACCCTAATCCATCCTTTTCCCTTTGTA19230.7322617864445892No Hit
GTCACCAACCCCATGTCTTTGGAGTGTTTGGACTTATGCCGCTTGTGCTT18320.6976097726294787No Hit
CTGTCCTTTTCCACAATCACACATATTCTTTCAAACATACTCTGCAGGTG17980.6846628663688878No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA17490.6660040896992129No Hit
GCTAACATGTTGCCAATCAGAGGATGTGATCACAATTCGTAATAAAGGAT17250.6568650970446782No Hit
CCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGCAAGTGGCTCCTTG16220.6176435869023004No Hit
ACCTGTATAGGGTATGCTCTTTGAATAATTACATCAACACAACCAACATT16100.613074090575033No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT16060.6115509251326106No Hit
TCTCCAGGTAATAAGGGTGGGAGGGGCAAAGGAGGCAACGTTGAAGTTTT15960.6077430115265544No Hit
TGCACGTACCACATTTAATTACCTTAGACTTGGTCTTCTCTTGGTATTAG15360.5848955298902179No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT15170.5776604940387112No Hit
GCCGGTTGTAAAGAGCCATGTATCATAACTGCTTGCGAAGATGTAGTTTC13790.525111286275137No Hit
GAGGAAAGTGCTTAATCTTATTTGGTTACTATGTTACCTGTTATTCAGCA13430.511402797293335No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG13270.5053101355236452No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG13010.4954095601478994No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG12630.4809394884448861No Hit
TTCTCTTCCGCACCCAGACTGGAATTAAGTGGAAGCCTGTAGAACCATGT12610.4801779057236749No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC12590.47941632300246373No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA12560.4782739489206469No Hit
AACAGATATGCTATAGTACAGAAGGCTACGGTACCTGCTGAATACAAGTT12410.4725620785115628No Hit
TCATTCAGAAAACAAACAGAGCACATAAAATGATACAGAAAAAGTACAGA12060.45923438089036633No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG11970.4558072586449159No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT11610.44209876966311384No Hit
AGAACATTTCCTCAGAATTGTCCCAAAAGAGCTAGTTAAGGACAAAGTTG11130.4238207843540446No Hit
CTTGCCCTAGATGTTCAGAGCTGCCTTCAGTATGATTATCTAGCAAGTTC11070.42153603619041086No Hit
CCCCAAAACACAGTAACCCAAATACTCACCATATTGTTAACATTCTTTAA10950.4169665398631435No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT10660.4059235904055809No Hit
TTCTTTGTAAGGCGTGTCTCAATATTTGACATTTTCATTTCTGTTGTAGT10590.4032580508813416No Hit
CGATAAAAGTGAACCTATTAATCTCTATAGTCTTTTTGTTTATAAAGGAT10550.40173488543891916No Hit
TGCCTGAATGTTCTTCACCACCTTCATGCAAACGTAAAGTTGGTGGTACA10350.39411905822680693No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT9590.3651789148207805No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA9260.35261279992079536No Hit
GGCAAGGACAGGGAAGGGGTCACTAGTGAAGAGCCCATGTTAAGTTGATT8520.3244342392359802No Hit
GGTGGCAGCAGGTAGTTTCTGCTACCGTAATGGAGATTTCTTGAAGTGAG8250.3141528724996287No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAATGGAG8150.3103449588935726No Hit
CTCAGTCTGTCTTGCCAGTGATACTAAACAATTCGACAGTTCAGGCAGCC8090.30806021072993894No Hit
TCCTCAGCAGTCTGGAACCCCGCAGACACCAGCTTCACCCGCACCGCTGG7890.30044438351782676No Hit
GGATCAGTATCATTTGGTTCCACTACACTGAGACATGGTATATCAGCAAA7860.29930200943600993No Hit
AGAGACATCTTAAAGAGGGAAGCTGTATGAAAACCCACAAATATGACTCA7840.2985404267147987No Hit
TCGCTCCGATCTGATCCACGACGTTCGTCGTTCTCCCTTCGGTCTAGTTT6920.2635076215390825No Hit
TTGACATACCTCAGCACACACATACACACAAAATGAATGATACAAACCTT6880.2619844560966601No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG6710.2555110029663647No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT6320.2406601399027459No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAACAGAAACCTTCATTT6310.24027934854214025No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA6250.2379946003785066No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT5930.2258092768391271No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG5910.22504769411791584No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT5890.22428611139670462No Hit
GTGTCAATGTACACCACAGCTCAAAGGAGGCTCTAGGTTTTGTAGAACCA5700.217051075545198No Hit
TGGCTTTGCATAATATGGCTTTAAAGTCAGTAAGACATGGTATATCAGCA5640.21476632738156434No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC5520.21019683105429704No Hit
TGGGAGGTGAGAGTTTAATAAAATAGTCAAGGACACATAGCATTAACTGA5370.2044849606452129No Hit
AGATTAGGGGTTTTGCAACCTGAGGTCTATAAACAAAGTCTTCCTGGAAG5370.2044849606452129No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC5260.2002962556785512No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG5250.19991546431794555No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT5170.19686913343310067No Hit
TCACTTTTTCTTCCTGTACACCTCTCTGATCACGAGATTTAAAACTATTA5060.19268042846643896No Hit
AAAATGTGATCCAAGAAACTTTTAGCACCAATGCAGAATTTATTTCAGTA5040.19191884574522772No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTACGACAG4370.16640582458465183No Hit
CCCCCTACTCCCTCTTATCCAAAGTCCTTGCTTTTCTTTTCCACCGCTTC4290.16335949369980693No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTACTCATAACTTTTTTTTGCACT4280.16297870233920134No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT4160.158409206011934No Hit
AGTGACGTGTCCTCTGTTCCTTCTCTTTCTTCACAGGTTTTGAAGGAAGA4150.15802841465132839No Hit
TTTCCAATTCCCTCCCAACCCCTCTATACTTAATATGGTAATTTCTTATA3880.1477470479149769No Hit
GAACGAAGCTCTATCTGCCCAATACTGTTTCTGCAAGTGTGTCATGTAGA3870.1473662565543713No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC3630.13822726389983664No Hit
TGGATTTACACACTAACCTGGTAGAAATGCGACTAAAGACTGCATTGAAG3540.13480014165438614No Hit
GTGACACTCTTGATGGCAGGAATGAAAATCTTCAGGAAAGTGAGATTCTA3530.13441935029378055No Hit
CCTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC3450.13137301940893564No Hit
TTAAAAGCAACAAACCCCAAATCAAACTGAAGAGAATTTTAAAAAACCTA3360.12794589716348517No Hit
GTGTCCAACCAATGCAAAGTTAAAGTTAGAGTTGAAATTGAGTCCAACAA3360.12794589716348517No Hit
ATTGGACCAGTCTACCCTGTAAAAATGTGATTCTTTGCTAGTACCTTTGT3360.12794589716348517No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT3200.12185323539379539No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT3160.12033006995137295No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT3130.11918769586955612No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCAAAACATGAATGTTCTCAAC2970.11309503409986635No Hit
TCCTTCTTTTTCCTACTGAGTTCAGCTCCCAGACTGGAATTAAGTGGAAG2850.108525537772599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)