FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91027
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC1025211.262592417634329No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC955710.499082689751392No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG90619.954189416327024No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA44184.853505004009799No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC41394.547002537708592No Hit
ATACCACTGACGGCCAGGTACACTGACGACATGGTTCTACAATACCACTG40774.478890878530546No Hit
TACTCTTCTTGGCTCCAGTTGCCACTATTTCTTCCCATTCACCGAGCCCC32863.6099179364364424No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT32483.5681720808111876No Hit
GGTAGGATGTGATATTCCTTCTAGTGGTACGGTAGCAGAAAGTCAGTCCC31103.4165687103826334No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA24692.71238204049348No Hit
TCAGCCTTAGCTTTTTACACAAGTCTCTGCTCCTAGCTAACATAATTGCA21722.3861052215276786No Hit
TGTCATAATAAGAACAATGAAAGTTGTACACTGACACAATGGCAACAGTT17891.9653509398310391No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA15861.74234018478034No Hit
GTCTCGGAGGCTGGCATCCAGCACACAGCTCAGAATACTAGTAGACCAAG15621.7159743812275479No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT14881.6346798202731057No Hit
TTACAATTTCTACCGCTGGTTCCACACTGACGACATGGTTCTACATGAAA11631.2776428971623803No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATAT11431.2556713942017204No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATA10391.1414195788062882No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC8920.979929032045437No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATATCGAT8820.9689432805651069No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT8580.9425774770123151No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC6440.7074823953332527No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATATCGA5700.6261878343788106No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA5660.6217935337866787No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC5050.5547804497566656No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATATC4950.5437946982763355No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC4370.48007733969042155No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATAT4220.4635987124699265No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT4090.44931723554549746No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA4030.44272578465729945No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG3840.42185285684467244No Hit
GAGATTGATGACCAAAAGAACTGCTACCGTATTCTCCTCAGATGACTCCA3390.3724169751831874No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATATCGA3070.3372625704461314No Hit
ATACCACTGGCCATTCTTTACTGCACAAGACCAAGTCTCTGCTACCGTAT3010.3306711195579333No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATATCG2990.32847396926186734No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT2960.3251782438177684No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATATC2950.32407966866973537No Hit
GGAAACACTGATGGGCCTGAAAGTCACACCACCTCAGCAACCAGACCAAG2630.2889252639326793No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT2360.2592637349357883No Hit
GTACAATTTCTACCGCTGGTTCCACACTGACGACATGGTTCTACATGAAA2330.2559680094916893No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT2150.23619365682709526No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC2110.23179935623496326No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT1930.21202500357036924No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG1910.20982785327430323No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATATCG1910.20982785327430323No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGATTTCAATGAATG1640.1801663242774122No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA1560.1713777230931482No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA1540.16918057279708218No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT1380.15160337042855418No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTATATCGATG1340.14720906983642215No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATATCGATGCTATC1180.12963186746789415No Hit
GTCTCGGAGGCTGGCATCCAGCACACAGCTCAGAATACTAGGAGACCAAG1070.11754754083953113No Hit
TACCCTCCATGAGGCACACATTGTTATTTCAGTTATATGGTACACATTGT1050.11535039054346513No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA1050.11535039054346513No Hit
CTGTGGTGCCACCTAAGCTCTGCTACCGTAACCAGGTTAGTGTGTAAATC1040.11425181539543212No Hit
GCTATTCCTACCATTCTGATGAGGTAGACCAAGTCTCTGCTACCGTATAT1030.11315324024739912No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC1020.11205466509936611No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTATA980.10766036450723412No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT970.10656178935920112No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT950.10436463906313512No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTATA940.10326606391510211No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)