FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91027
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG969610.65178463532798No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG958810.533138519340415No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT89959.881683456556846No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT43834.815054873828644No Hit
TGTGCAGTAAAGAATGGCCAGTGGTATTGTAGAACCATGTCGTCAGTGTA41934.6063255957023745No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA41284.534918211080229No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC32353.5538906038867593No Hit
TACTGTAGGGGCTCGGTGAATGGGAAGAAATAGTGGCAACTGGAGCCAAG31173.424258736418865No Hit
CCATGGGACTGACTTTCTGCTACCGTACCACTAGAAGGAATATCACATCC30653.3671328287211484No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT24782.7222692168257767No Hit
GCAATTATGTTAGCTAGGAGCAGAGACTTGTGTAAAAAGCTAAGGCTGAT21802.3948938227119427No Hit
CAGAACTGTTGCCATTGTGTCAGTGTACAACTTTCATTGTTCTTATTATG17881.9642523646830061No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT15791.734650158744109No Hit
ACTAGTATTCTGAGCTGTGTGCTGGATGCCAGCCTCCGAGACTGTAGAAC15631.7170729563755809No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT13811.5171322794335746No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTGGAAC13731.5083436782493107No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA11501.2633614202379513No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG10521.1557010557307172No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC8820.9689432805651069No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG8770.963450404824942No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA8220.9030287716831269No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG6390.7019895195930878No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT5720.6283849846748767No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT5710.6272864095268437No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG5110.5613719006448636No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT4960.5448932734243686No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG4330.4756830390982896No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA4230.46469728761795953No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA4120.45261296098959647No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG3930.4317400331769695No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG3800.41745855625254047No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT3720.4086699550682764No Hit
TTAAATGGAGTCATCTGAGGAGAATACGGTAGCAGTTCTTTTGGTCATCA3210.35264262251859335No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT3080.33836114559416436No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT2970.32627681896580135No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG2930.3218825183736694No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC2870.31529106748547137No Hit
CGTGCAGTAAAGAATGGCCAGTGGTATTGTAGAACCATGTCGTCAGTGTA2710.2977138651169433No Hit
GGTTGCTGAGGTGGTGTGACTTTCAGGCCCATCAGTGTTTCCTGTAGAAC2690.2955167148208773No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT2310.25377085919562326No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC2170.23839080712316127No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT1980.21751787931053426No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC1960.21532072901446825No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT1950.21422215386643523No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC1550.1702791479451152No Hit
CACTGTAGGGGCTCGGTGAATGGGAAGAAATAGTGGCAACTGGAGCCAAG1450.1592933964647852No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT1370.15050479528052116No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG1340.14720906983642215No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT1210.13292759291199313No Hit
GTTGATACAAAACAAGCCCACGAACTGGCTAAGAGTTACGGGATTCCATT1170.12853329231986116No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT1070.11754754083953113No Hit
ACCTCATCAGAATGGTAGGAATAGCTGTAGAACCATGTCGTCAGTGTAGA1070.11754754083953113No Hit
CCTAGTATTCTGAGCTGTGTGCTGGATGCCAGCCTCCGAGACTGTAGAAC1050.11535039054346513No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA1020.11205466509936611No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG1000.10985751480330012No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT990.10875893965526713No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG970.10656178935920112No Hit
CATATTAGAAATAACAATGTGTACCATATAACTGAAATAACAATGTGTGC960.10546321421116811No Hit
TGGATTTACACACTAACCTGGTTACGGTAGCAGAGCTTAGGTGGCACCAC930.1021674887670691No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)