FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115618
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT1828515.815011503399123No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG1626714.06960853846287No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC91007.870746769534155No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC67785.862408967461814No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT41963.629192686259925No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC38323.314362815478559No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA35043.0306699648843605No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC34783.0081821169714056No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG24692.135480634503278No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA17091.4781435416630628No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT15871.37262363991766No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA15101.306025013406217No Hit
TGTCATAATGAATGCCCCATCGCCTTCGGAGAGAATTACCCGAGACACGG14241.2316421318479822No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG13971.208289366707606No Hit
GATTCCCGCGCCCAGAGCAGACGTGGGAGGAAGGAAAGAGCATTCAAAGT12411.0733622792298776No Hit
GGAAACACTGATGGGCCTGAAAGTCACACCACCTCAGCAACCAGACCAAG10550.9124876749295092No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT10180.8804857375149198No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT8610.74469373280977No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC8600.7438288155823487No Hit
GTGCTCAAAAGAAGTGCTGAAGGTACGGTAGCAGAAGCCTTTTTAGCCCT7840.6780951062983273No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC7080.6123613970143057No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC7010.6063069764223563No Hit
ATAGAATTTTCATATTGATTAGGCTGTTCCTTGCTGCAACTTTTATCCCA6970.6028473075126711No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT6870.5941981352384577No Hit
TATTTAGTAGCCAGGACAGTAGAAGGACTGAAGAGTGAGAGGAGCTCCCA6570.5682506184158176No Hit
GACAGCAGAACACACATACCAAAAACACCCATCGTTCTCTGCTAAGACCA6510.5630611150512896No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA6340.5483575221851269No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACAT6280.5431680188205988No Hit
GTCTCGGAGGCTGGCATCAACATAGGCACACAGCTCAGAATACTAGTAGA6270.5423031015931775No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA5550.4800290612188413No Hit
AGCCTGCTGACTACCTTAATTGGCTTGGTCTCACCATCCAAAAGACCTAT5020.43418844816551055No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA4950.4281340275735612No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA4860.4203497725267692No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAATTCTTTAACTTACTAAAA4390.3796986628379664No Hit
AGATGATACTGCTGCCCTCGAGTCCCACGACCCCCTCAGACCAAGTCTCT4310.37277932501859573No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC4250.3675898216540677No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT4080.352886228787905No Hit
ATTTGTTGCAGGTTTTGGTTTTAATGGCTTGTGGCGGTTTGCAGGACCGT3980.34423705651369163No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC3820.33039838087495027No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACATGCAT3670.31742462246363023No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC3400.29407185732325414No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC3100.26812434050061407No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG3080.2663945060457714No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGAAAATAATCACTCTATTAAAGTTT2730.23612240308602464No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC2510.21709422408275528No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACA2500.21622930685533392No Hit
GCCACAAAGTAAAAATGTTGTGTGTTTACTTTTTTGCATCTTGGCAGGCT2480.21449947240049128No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACAT2360.20412046567143527No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACATG2340.20239063121659256No Hit
AAACTATAAGATAAGGAATCCAGCAAAGGCCAATCAAGTGAAGAAAGACC2290.1980660450794859No Hit
GTGCAGGCTGATTTTCTTTTTCCACGACATGGTTCTACAGTGCATGTGCC2280.19720112785206453No Hit
TACTGAATGCAAAGGACACCACAACAGGGTTCCAGACTGCTGAGGAAGAC2230.19287654171495788No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACATGCA2100.18163261775848052No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACATG2010.1738483627116885No Hit
CCTGTTTCCGCCACCTCTGTCACTGCTAGAACAACTATCAATTTGAGACC1860.16087460430036846No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG1860.16087460430036846No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC1770.15309034925357642No Hit
AGGTGTGTGTGTGGCTTCAAAACACTGACGACATGACCTTCAAGTATTAG1740.15049559757131242No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC1730.1496306803438911No Hit
TGATAGAATCTGATGAAGAATGTGCTGCATATATTTTACCAAAACACAAA1700.14703592866162707No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTACATGCA1670.14444117697936307No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC1600.13838675638741374No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACATGC1580.13665692193257106No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTACATGC1390.12022349461156567No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT1350.11676382570188033No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG1320.11416907401961632No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGTTGAGGTTTCAATGAATG1290.11157432233735232No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)