FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115618
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT1666714.415575429431405No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT1618513.99868532581432No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG90387.817121901434032No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA67845.8675984708263424No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT41123.556539639156533No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA36133.124945942673286No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA35113.03672438547631No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT33072.8602812710823575No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC25252.183915999238873No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA16731.4470065214758947No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA14841.2835371654932624No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG14601.2627791520351503No Hit
CTGGGATAAAAGTTGCAGCAAGGCCGTGTCTCGGGTAATTCTCTCCGAAG14271.2342368835302462No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC12971.1217976439654724No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT12461.0776868653669844No Hit
ACACTTTGAATGCTCTTTCCTTCCTCCCACGTCTGCTCTGGGCGCGGGAA11490.9937898943071147No Hit
GGTTGCTGAGGTGGTGTGACTTTCAGGCCCATCAGTGTTTCCTGTAGAAC10620.9185420955214586No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT10180.8804857375149198No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT8170.7066373748032313No Hit
AGGGCTAAAAAGGCTTCTGCTACCGTACCTTCAGCACTTCTTTTGAGCAC7660.6625265962047432No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC7590.6564721756127939No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG6990.6045771419675137No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT6910.597657804148143No Hit
CTGGGATAAAAGTTGCAGCAAGGAACAGCCTAATCAATATGAAAATTCTA6900.5967928869207216No Hit
TAGCAGAGAACGATGGGTGTTTTTGGTATGTGTGTTCTGCTGTCTGTAGA6460.5587365289141829No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG6340.5483575221851269No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA6330.5474926049577056No Hit
ACTAGTATTCTGAGCTGTGTGCCTATGTTGATGCCAGCCTCCGAGACTGT6310.5457627705028629No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA6260.5414381843657562No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA6230.5388434326834922No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT5280.45667629607846527No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT4870.4212146897541905No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT4720.40824093134287054No Hit
ACCTGGATATAGGTCTTTTGGATGGTGAGACCAAGCCAATTAAGGTAGTC4710.40737601411544916No Hit
GAGGGGGTCGTGGGACTCGAGGGCAGCAGTATCATCTTGTAGAACCATGT4340.37537407670085976No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT4110.355480980470169No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA4030.3485616426507983No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAGTGCAAACAATACATCATACTT3910.3381826359217423No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG3760.3252088775104222No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAACAGAAACCTTCATTT3650.3156947880087876No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC3630.3139649535539449No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG3370.29147710564099016No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC2910.2516909131796087No Hit
CTGAGTAAGTGGCAAGAAAATTACCTGCGTGCTGCATCAAAGTTGCTTTT2860.247366327042502No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG2560.22141881021986196No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG2520.21795914131017663No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCAAAACATGAATGTTCTCAAC2450.21190472071822727No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA2360.20412046567143527No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT2350.20325554844401392No Hit
TTCTTCACTTGATTGGCCTTTGCTGGATTCCTTATCTTATAGTTTTGTAG2300.19893096230690724No Hit
ATTAGGATGTCTGGCACATGCACTGTAGAACCATGTCGTGGAAAAAGAAA2240.1937414589423792No Hit
TCCTCAGCAGTCTGGAACCCTGTTGTGGTGTCCTTTGCATTCAGTATGTA2220.19201162448753653No Hit
TCAAAAGGAGATACTTACACAACAGGAAAGACTTTGGGAAACACAACACT2160.18682212112300853No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT2100.18163261775848052No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT2060.17817294884879517No Hit
CAAATTGATAGTTGTTCTAGCAGTGACAGAGGTGGCGGAAACAGGTGTAG1860.16087460430036846No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT1860.16087460430036846No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA1810.15655001816326178No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA1740.15049559757131242No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT1720.14876576311646977No Hit
GTAAAACCCACTAATACTTGAAGGTCATGTCGTCAGTGTTTTGAAGCCAC1710.14790084588904842No Hit
GGATTACTCTTAGATTTGTGTTTTGGTAAAATATATGCAGCACATTCTTC1690.14617101143420574No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC1560.13492708747772839No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGAATTCCATT1520.13146741856804303No Hit
GCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT1500.12973758411320035No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCC1430.123683163521251No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1420.12281824629382969No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG1350.11676382570188033No Hit
CCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA1340.11589890847445898No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT1340.11589890847445898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)