FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136490
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC1448810.614697047402741No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC106187.779324492636823No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC65884.826727232764306No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG63394.644296285442158No Hit
CTGTATGTAGTCGGTGCTGTGAGTAACAGGTAGATGTCATTTCTGAGACC62324.565902263902117No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA39772.913766576305956No Hit
CAGTAGAGGCTAAGGATTCAGGTACACTGACGACATGGTGGTTGATCTTA34502.5276577038610886No Hit
GGCCTGCCCAGGTTGTGCTGTTTGGGGAACCATACTTTGAGAAACACACT32392.3730676240017585No Hit
TTCTGATGAGCCAAGTCTCTGTGAGACAGGTGAGTCATTCAGTAATCACA31642.318118543483039No Hit
CTGGCTGAGCACATAGAGCAATGGAACAGAATAGAGAGCCCAGAAATGAG18321.3422228734705839No Hit
CGATGCGCGGGAAGACGGAAGGCAGTCTGGCTGAGACCAAGTCTCTGCTA14101.0330427137519231No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC13310.9751630156055389No Hit
GCTTAGATTCCTCTTTGGAGTCAGTGTAGCAAGTCATGGTAAGTCCTCTG13300.9744303611986227No Hit
ACTTCTACCGTGCCCTGAGCCAGAAGGGAGCCCACTTCATTAGTACTGGA13110.9605099274672136No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA12390.9077588101692432No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT12330.9033628837277455No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT12310.9018975749139131No Hit
GCCTACCAGTCCAGCACCACCTCCAGAACCAGCAACAGACCTGCCCACAC11040.8088504652355484No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACTA10930.8007912667594695No Hit
GCCGGAACAGCCCCAGGCCCCTGAGTTTAAGAACCGCCCGGGTGGGATGT10270.7524360759029965No Hit
ATGAGCAAGTTGGGGTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGC9070.6645175470730456No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT8940.6549930397831343No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA8380.613964392995824No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACT8340.6110337753681588No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA7980.5846582167191736No Hit
GGGATAAAAGCACAAAGGTCTGCTTCTTTTTACCTTCAGAGCTAGGGAAA7870.5765990182430948No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACTAGATC7770.5692724741739321No Hit
GCCTACCAGTCCAGCACCTCACACCACCTCAGCAACCAGACCAAGTCTCT7410.5428969155249469No Hit
ATGAGCAAGTTGGGGTGTGCGCTTGTGGGGGTTGTTGACTTGTAGACCAA7100.520184628910543No Hit
GATTCCCGCGCCCAGAGATGCCGACGAGTACCTCATAGACCAAGTCTCTG6930.5077295039929666No Hit
ACAGCTTCTGCCATCTCTCTCACTGACGACATGGTTCTACAACTGCTATT6850.5018682687376365No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA6640.486482526192395No Hit
CTAATGAGCGGACGGCAAAGAACAGAAAGTCAGTCCCATGGAGACCAAGT6190.4535130778811634No Hit
TACCCTCCATGAGGCACACAGAGAGGAGAGGCTATGGGGAGACAAAAACA6130.4491171514396659No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC5590.40955381346618797No Hit
GCAGTAAAGCAGGCAATATCTGGTGGGGTTCGCTCATTATTTCCTAGACC5450.39929665176936036No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA5430.3978313429555279No Hit
GCTATGCATCTACCACCCTCACCTCCAAGTGTGAGTGACTGGTGGAGACC5300.3883068356656165No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC5030.3685251666788776No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT5010.3670598578650451No Hit
CCTCCCCCACCCCCTCTACACTGACGACATGGTTCTACTCACTCAAAGAC4890.35826800498204997No Hit
AGATGAGTCATATTTGTGGGTTTTCATACAGCTTCCCTCTTTAAGATGTC4860.3560700417613012No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC4680.3428822624368086No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACTAG4590.33628837277456225No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACTA4480.3282291742984834No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC4380.32090263022932086No Hit
CCAGTGTTGTGTTTCCCAAAGTGCTGGGATTACAGATGTGCCACCATACC3960.290131145138838No Hit
GCCTACCAGTCCAGCACCCAGATCCCCCAACTACCTCAGGGACAAGTACT3670.26888416733826653No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACTAGAT3550.2600923144552715No Hit
GCGTGGACAACCCCCACTAGGTTCCAGGGTGGAGGGGAGATAAGGAGCCA3530.258627005641439No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG3150.23078613817862115No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACTAG3100.22712286614403987No Hit
GCCTACCAGTCCAGCACCATGAATCCCACCTAGGGTTCCAAGGCCCCTGC3020.2212616308887098No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG2900.2124697780057147No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTACTAGAT2890.21173712359879845No Hit
GGAAACCGTAGCTGCCCTGTCACACCACCTCAGCAACCAGACCAAGTCTC2820.20660854275038465No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA2790.20441057952963587No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT2750.20147996190197087No Hit
TCGGCTATAAAAAAGATAATGGAAAGGCGGGGAAGAAAAGTGCCAGACCA2620.19195545461205948No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACTAGA2430.1780350208806506No Hit
GTTAGAAGGCTGGCTCCCCGAACTGTGTCTTCCTCAACTCTAGGAAGGAC2430.1780350208806506No Hit
CCATTTTCTTCACACCTGTCGTCAGTGTACCAGGTTAGTGTGTAAATCCA2330.17070847681148804No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC2260.16557989596307424No Hit
CTGGTTCAGAGGAAGTATCATGTCGTCAGTGTACTGGCCATTCTTTACTG2210.16191662392849293No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT2180.15971866070774415No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC2170.1589860063008279No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA2120.1553227342662466No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTACTAGA2040.14946149901091654No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA1970.14433291816250274No Hit
TTGTCAGATTTAACTTTTTTGGAAGTTGTACGGTAGCAGAGCGAGGTAAT1880.13773902850025643No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT1860.1362737196864239No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT1820.13334310205875888No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG1740.12748186680342882No Hit
GCAGCTTCTGCCATCTCTCTCACTGACGACATGGTTCTACAACTGCTATT1720.1260165579895963No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA1710.12528390358268005No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGTTGAGGTTTCAATGAATG1700.1245512491757638No Hit
TTTCATATAGGCTGTGCAACAAGTCTCTGCTACCGTACCAACTCCCTGGC1510.1106308154443549No Hit
TTCTGATGAGCCAAGTCTCTGTGGTGAATTTGGGAGGAGGGCAGTACACA1470.10770019781668988No Hit
GAGAAAGCCCCCCTACTGCTGTTCCCGCTACAAAGACTGCCTTTTCCCTT1450.10623488900285735No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT1440.10550223459594109No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTACTAGATCT1440.10550223459594109No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC1400.10257161696827606No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA1390.1018389625613598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)