FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136490
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG1452810.644003223679391No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA106067.770532639753829No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT63764.671404498498059No Hit
CAGAAATGACATCTACCTGTTACTCACAGCACCGACTACATACAGTGTAG62454.575426771192029No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG60944.4647959557476735No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA40032.932815590885779No Hit
CACTTGCTGAGTGTGTTTCTCAAAGTATGGTTCCCCAAACAGCACAACCT31782.328375705179867No Hit
ATGTTACCTTAAGATCAACCACCATGTCGTCAGTGTACCTGAATCCTTAG30222.2140816177009306No Hit
TTGTGATTACTGAATGACTCACCTGTCTCACAGAGACTTGGCTCATCAGA28882.115905927174152No Hit
CCAGACACTAAGAGCAGGAATGTTCCCTCATTTCTGGGCTCTCTATTCTG17891.3107187339731847No Hit
CAGCCAGACTGCCTTCCGTCTTCCCGCGCATCGTGTAGAACCATGTCGTC14071.0308447505311744No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG13260.9714997435709576No Hit
CCAGTACTAATGAAGTGGGCTCCCTTCTGGCTCAGGGCACGGTAGAAGTT12980.9509854201773024No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT12520.9172833174591545No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC12320.9026302293208295No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT12270.898966957286248No Hit
ACAGAGGACTTACCATGACTTGCTACACTGACTCCAAAGAGGAATCTAAG11350.8315627518499523No Hit
GGTTGCTGAGGTGGTGTGAGGATGGGTGTGGGCAGGTCTGTTGCTGGTTC11030.8081178108286321No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA10960.8029892299802184No Hit
TCTACCAGGAAAATCACATCCCACCCGGGCGGTTCTTAAACTCAGGGGCC9160.6711114367352919No Hit
ACAAGTCAACAACCCCCACACCCCAACTTGCTCATTGTAGAACCATGTCG9000.6593889662246318No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT8890.651329767748553No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG8530.6249542090995678No Hit
GTTATCAGCTTGTCAAGAATAGCAGTTGTAGAACCATGTCGTCAGTGAGA8490.6220235914719028No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT8430.6176276650304051No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC7740.5670745109531834No Hit
GGTTGCTGAGGTGGTGTGAGGTGCTGGACTGGTAGGCTGTAGAACCATGT7410.5428969155249469No Hit
ACAAGTCAACAACCCCCACAAGCGCACACCCCAACTTGCTCATTGTAGAA7210.5282438273866218No Hit
ATGAGGTACTCGTCGGCATCTCTGGGCGCGGGAATCTGTAGAACCATGTC6920.5069968495860502No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT6770.4960070334823064No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT6720.4923437614477251No Hit
ACTTTTTCCCTAGCTCTGAAGGTAAAAAGAAGCAGACCTTTGTGCTTTTA6620.48501721737856257No Hit
CCATGGGACTGACTTTCTGTTCTTTGCCGTCCGCTCATTAGTGTAGAACC6160.45131511466041474No Hit
GCAGATGTCTTTGAGTGAGTAGAACCATGTCGTCAGTGTAGAGGGGGTGG6090.4461865338120009No Hit
AGGAAATAATGAGCGAACCCCACCAGATATTGCCTGCTTTACTGCTGTAG5550.40662319583852297No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG5540.40589054143160674No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT5460.40002930617627663No Hit
TTCTGGGGCTGTTTTTGTCTCCCCATAGCCTCTCCTCTCTGTGTGCCTCA5410.39636603414169536No Hit
CCACCAGTCACTCACACTTGGAGGTGAGGGTGGTAGATGCATAGCTGTAG5330.3905047988863653No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG5040.36925782108579386No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT5010.3670598578650451No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG4660.3414169536229761No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT4640.3399516448091435No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA4560.33409040955381347No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG4420.32383324785698586No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA4300.31504139497399075No Hit
AGAGACATCTTAAAGAGGGAAGCTGTATGAAAACCCACAAATATGACTCA4290.3143087405670745No Hit
CTGGGTATGGTGGCACATCTGTAATCCCAGCACTTTGGGAAACACAACAC3850.2820719466627592No Hit
CAGTACTTGTCCCTGAGGTAGTTGGGGGATCTGGGTGCTGGACTGGTAGG3700.2710821305590153No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT3600.26375558648985276No Hit
GGCTCCTTATCTCCCCTCCACCCTGGAACCTAGTGGGGGTTGTCCACGCT3550.2600923144552715No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT3080.22565755733020737No Hit
GAGGGGGTCGTGGGACTCGGGGAATGGGTGAGCAGGGGCCTTGGAACCCT3000.2197963220748773No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT2900.2124697780057147No Hit
GGTTGCTGAGGTGGTGTGACAGGGCAGCTACGGTTTCCTGTAGAACCATG2820.20660854275038465No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG2810.20587588834346837No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT2780.2036779251227196No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT2770.20294527071580337No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG2720.19928199868122207No Hit
GGCACTTTTCTTCCCCGCCTTTCCATTATCTTTTTTATAGCCGATGTAGA2510.18389625613598065No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC2480.18169829291523187No Hit
CTGTTACCTTAAGATCAACCACCATGTCGTCAGTGTACCTGAATCCTTAG2440.17876767528756685No Hit
GGATCATTTTCACACTGTCCTTCCTAGAGTTGAGGAAGACACAGTTCGGG2360.1729064400322368No Hit
TGGATTTACACACTAACCTGGTACACTGACGACAGGTGTGAAGAAAATGG2300.16851051359073924No Hit
CTGTGATTACTGAATGACTCACCTGTCTCACAGAGACTTGGCTCATCAGA2230.16338193274232543No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA2160.15825335189391165No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA2160.15825335189391165No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA2150.15752069748699538No Hit
TGTGCAGTAAAGAATGGCCAGTACACTGACGACATGATACTTCCTCTGAA2090.15312477104549785No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG2050.1501941534178328No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC2040.14946149901091654No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT1820.13334310205875888No Hit
AATGCAGATGATTACCTCGCTCTGCTACCGTACAACTTCCAAAAAAGTTA1760.12894717561726135No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT1730.12674921239651255No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT1720.1260165579895963No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT1680.12308594036193128No Hit
GTGTTACCTTAAGATCAACCACCATGTCGTCAGTGTACCTGAATCCTTAG1590.11649205069968495No Hit
GAAAGCCAGGGAGTTGGTACGGTAGCAGAGACTTGTTGCACAGCCTATAT1550.11356143307201993No Hit
GTGTACTGCCCTCCTCCCAAATTCACCACAGAGACTTGGCTCATCAGAAT1480.10843285222360613No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT1450.10623488900285735No Hit
TTGTGAAGAGAACATGATTGGCAATACTCTGAAGTATCTACACAATAAAA1390.1018389625613598No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA1380.10110630815444355No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG1380.10110630815444355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)