FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145708
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC81755.6105361407747No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC75785.2008125840722546No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAAAGA64994.460290443901502No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC64664.437642408103879No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG64554.430093062838004No Hit
TCCTCTCTTAGATTTGGCTTTCTGTCGCGTAGGCAACCAGTGACACCTTT62104.261948554643533No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC57983.9791912592307903No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT56273.8618332555522No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC25691.7631152716391687No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG25261.7336041946907514No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA24401.6745820407939167No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA22921.5730090317621546No Hit
TGATTCTCCACTTCATGCTGTCAATTCAGAGCTGCAGAAACACAATTTGA21321.4632003733494385No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA20971.439179729321657No Hit
GCAGAGCCAAACCGACCTGTGAAAAGGATGGCTAACAGGAGACCAAGTCT19641.347901282016087No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA18051.2387789277184507No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGCG16601.1392648310319269No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC16071.1028907129327148No Hit
TGTTTCAGGAAGGAATGTTCCCACTTATGGGTGAAAGGGCTAGGACTAGA15111.0370055178850852No Hit
GCCGGAACAGCCCCAGAGACCAAGTCTCTGCATTCCCGCGCCCAGAGACC14380.9869053174842836No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT13890.9532764158453894No Hit
TGTCATAATGAATGCCCCATCGCCAGTTTGTCCTCTTAATGGCTGTGAAG13090.8983720866390315No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGCGTAGA11520.7906223405715541No Hit
AGAAGACCAGGAAAGAACGGGAGCTTCTTATGGCCAAGAGACCAAGTCTC10290.7062069344167787No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA10170.6979712850358251No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGTGCTTTTCCCAACACCACC10140.6959123726905867No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC9790.6718917286628051No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGC9120.6259093529524803No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC8980.6163010953413677No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGCGTAG8860.608065445960414No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA8620.5915941471985066No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA8510.5840448019326324No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC8420.5778680648969171No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC8370.5744365443215198No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA8230.5648282867104071No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC8090.5552200290992946No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGCGT7680.527081560381036No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT7480.5133554780794466No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC6970.4783539682103934No Hit
TAAAGGAACTGTAGTCGCCCTGTACGGCAGCAGAAGCGCTGATCAGAAAG6730.461882669448486No Hit
TGTATCAAAAATACTTCCTCGTGTTGTGGGGGTTGTTGACTTGTAGACCA6090.4179592060833997No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG5590.38364400032942597No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT5570.382271392099267No Hit
TCTAAGCATAGCATTCAATTTTGGCTACCGTACTGTTATTACGGCTAATT4910.33697532050402174No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGCG4780.32805336700798854No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT4530.31089576413100173No Hit
CCCAGCTGAGATCTTACCAGATACACTGACGACATGGTTCTACACCCAGC4510.3095231559008428No Hit
GATTCCCGCGCCCAGAGGACATGGTTCTACAGAGTCCCACGACCCCCTCA4480.30746424355560437No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG4220.28962033656353803No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGCTGTGTCTACTCATTTGAA4130.2834435995278228No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT3960.2717764295714717No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT3680.2525599143492464No Hit
GTTAACTGCTACTAAAACGGAGCCATGTGTAGAACCATACCAGAAGAATT3680.2525599143492464No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT3660.25118730611908746No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAGAGA3600.24706948142861063No Hit
CCCCTGCTCCTATAGCCAAGTCTCTGTGAGTCCCACGACCCCCTCAGACC3550.2436379608532133No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT3550.2436379608532133No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGCGTA3520.24157904850797488No Hit
AGATGAGTCATATTTGTGGGTTTTCATACAGCTTCCCTCTTTAAGATGTC3510.24089274439289537No Hit
GGCTTCCACTTAATTCCAGTCTGGGTGCGGAAGAGAAAGACCAAGTCTCT3450.23677491970241854No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCAGAGAC3420.2347160073571801No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT3340.22922557443654434No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG3040.20863645098416012No Hit
GGCTTCCACTTAATTCCAGTCTGTACGGTAGCAGAACTGTCAAATGACCA3020.20726384275400114No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGCGT2980.20451862629368325No Hit
ATGAGCAAGTTGGGGTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGC2930.20108710571828586No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAGCGTAG2770.1901062398770143No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC2740.1880473275317759No Hit
AGAAATGTATAACCAAGTCAGTAAGGCACACTGACGACAGGTTTCTTAGA2730.1873610234166964No Hit
GCCCTCTCAGAAACATCTGCTACCGTAGCTTCTCAGTGGTGTTCAAATCA2710.18598841518653747No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT2610.17912537403574272No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT2590.17775276580558377No Hit
GTCTCGGAGGCTGGCATCGTCAGTGTAGAACATCGTCAAAAAATTACTTC2590.17775276580558377No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC2490.17088972465478902No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC2450.16814450819447113No Hit
AATGAAGAACTTGCTAGATAATCATACTGACGACATGGTTCTACAAATGA2270.1557910341230406No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGCGTA2260.15510473000796113No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG2200.1509869053174843No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT2190.15030060120240482No Hit
TCCACAAACAGAACAAGATGCTACCGTAATCCTGTTTGAAGGAAAAGGCC2130.146182776511928No Hit
CTGGTTCAGAGGAAGTATCATGTCTCTGCTACCGTAACTGGCCATTCTTT2110.14481016828176901No Hit
GGGAAAAGAACAGGCTTCACCTATCATCGTTTTCCAAGTTATTTTTGAGA2110.14481016828176901No Hit
AACTGAGCGACCTCAGAGCCCTCTTGCTCATGGTGGGGGCAGCAGACCAA2100.14412386416668954No Hit
GAATTGTGCAGAGTGTGGTGTACCCTTTCGGTAAGACATGTAGACCAAGT2090.14343756005161007No Hit
GTAGAGGAGCCGTCAAATCCATACGGTAGCAGCATCTTTCCAGTTCGTAT2040.14000603947621268No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA2000.13726082301589482No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGCGTAGACGAATC1950.13382930244049743No Hit
CACCCTTGAACAGTAAAAGCGAAGACCAAGTCTCTGCTACCGTAGCGTAG1880.12902517363494112No Hit
CCATTTTCTTCACACCTGTCGTAAGTAGTTCACAGAATGTTATTTTTCAG1690.1159853954484311No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA1670.11461278721827217No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT1640.11255387487303374No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAGAGACCAAGTC1640.11255387487303374No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA1640.11255387487303374No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAGCGTAGAC1570.1077497460674774No Hit
GGATCCTGGGTGTTTGTATTTGCTACCGTAAGGTGAGTCATTCAGTAATC1490.10225931314684163No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG1480.10157300903176215No Hit
TTCTGATGAGCCAAGTCTCTGTGAGACCAGGTGAGTCATTCAGTAATCAC1460.1002004008016032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)