FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098924

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098924
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145708
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG86575.941334724243006No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG80785.543964641611991No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA64554.430093062838004No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT64094.398523073544349No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT62784.308617234468938No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA57673.9579158316633265No Hit
TGTGAGTGATAAAGGTGTCACTGGTTGCCTACGCGACAGAAAGCCAAATC57043.91467867241332No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC55813.830263266258545No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA25661.7610563592939306No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT24151.6574244379169298No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG22681.5565377330002472No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT22571.5489883877343729No Hit
GTCAAATTGTGTTTCTGCAGCTCTGAATTGACAGCATGAAGTGGAGAATC21161.452219507508167No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG20151.3829027918851402No Hit
CCTGTTAGCCATCCTTTTCACAGGTCGGTTTGGCTCTGCTGTAGAACCAT19731.3540780190518023No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT18201.2490734894446427No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA16631.1413237433771655No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA15951.0946550635517611No Hit
AGTCCTAGCCCTTTCACCCATAAGTGGGAACATTCCTTCCTGAAACATGT15031.0315150849644494No Hit
CTGGGCGCGGGAATGCAGAGACTTGGTCTCTGGGGCTGTTCCGGCTGTAG14050.9642572816866609No Hit
TCACAGCCATTAAGAGGACAAACTGGCGATGGGGCATTCATTATGACATG12970.8901364372580777No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT12030.8256238504406074No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC11540.7919949488017131No Hit
CTTGGCCATAAGAAGCTCCCGTTCTTTCCTGGTCTTCTTGTAGAACCATG10190.699343893265984No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG9780.6712054245477256No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG9090.6238504406072418No Hit
CTGTCCCACTGGAAAACTGGTAAGATCTCAGCTGGGTGTAGAACCATGTC9050.6211052241469239No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG8960.6149284871112087No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT8940.6135558788810498No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT8820.6053202295000961No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG8510.5840448019326324No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG8410.5771817607818377No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT8160.5600241579048507No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG8130.5579652455596124No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT7710.5291404727262744No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT7510.515414390424685No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG6970.4783539682103934No Hit
TTCTGATCAGCGCTTCTGCTGCCGTACAGGGCGACTACAGTTCCTTTATG6620.45433332418261185No Hit
ACAAGTCAACAACCCCCACAACACGAGGAAGTATTTTTGATACATGTAGA6250.4289400719246712No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA5620.3857029126746644No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT5420.37197683037307494No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG5300.3637411809921212No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA4930.3383479287341807No Hit
GTGAGCACAATTAGCCGTAATAACAGTACGGTAGCCAAAATTGAATGCTA4840.33217119169846543No Hit
CGTGAGTGATAAAGGTGTCACTGGTTGCCTACGCGACAGAAAGCCAAATC4450.30540533121036595No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC4440.3047190270952865No Hit
GAGGGGGTCGTGGGACTCTGTAGAACCATGTCCTCTGGGCGCGGGAATCT4420.30334641886512753No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT4190.2875614242182996No Hit
GTTCAAATGAGTAGACACAGCTTACGGTAGCAGAGAGATGGCAGAAGCTG4140.28412990364290225No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC3920.2690312131111538No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT3700.2539325225794054No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG3680.2525599143492464No Hit
CTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT3630.24912839377384904No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC3560.24432426496829276No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT3550.2436379608532133No Hit
GAGGGGGTCGTGGGACTCACAGAGACTTGGCTATAGGAGCAGGGGTGTAG3490.23952013616273643No Hit
TTCTCTTCCGCACCCAGACTGGAATTAAGTGGAAGCCTGTAGAACCATGT3480.23883383204765696No Hit
AAGGAAAAGTTATGCAATTCTTCTGGTATGGTTCTACACATGGCTCCGTT3480.23883383204765696No Hit
CTGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA3350.22991187855162382No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT3350.22991187855162382No Hit
AGAGACATCTTAAAGAGGGAAGCTGTATGAAAACCCACAAATATGACTCA3170.21755840448019326No Hit
TGGTCATTTGACAGTTCTGCTACCGTACAGACTGGAATTAAGTGGAAGCC2970.20383232217860378No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT2960.2031460180635243No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT2940.20177340983336534No Hit
ACAAGTCAACAACCCCCACACCCCAACTTGCTCATTGTAGAACCATGTCG2940.20177340983336534No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT2800.19216515222225272No Hit
GATTTGAACACCACTGAGAAGCTACGGTAGCAGATGTTTCTGAGAGGGCT2720.18667471930161691No Hit
CAGGACTAGCTCTAAGAAACCTGTCGTCAGTGTGCCTTACTGACTTGGTT2710.18598841518653747No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA2700.185302111071458No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT2580.1770664616905043No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA2490.17088972465478902No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG2470.16951711642463008No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT2360.16196777115875588No Hit
ACATGGCCTTGCTGAAGTAATTTTTTGACGATGTTCTACACTGACGATGC2360.16196777115875588No Hit
TGTGCAGTAAAGAATGGCCAGTTCTTCATTTGTAGAACCATGTCGTCAGT2320.15922255469843796No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC2270.1557910341230406No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG2190.15030060120240482No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT2190.15030060120240482No Hit
CAAAAATAACTTGGAAAACGATGATAGGTGAAGCCTGTTCTTTTCCCTGT2120.1454964723968485No Hit
GGCCTTTTCCTTCAAACAGGATTACGGTAGCATCTTGTTCTGTTTGTGGA2110.14481016828176901No Hit
GCTGCCCCCACCATGAGCAAGAGGGCTCTGAGGTCGCTCAGTTTGTAGAA2090.14343756005161007No Hit
ACATGTCTTACCGAAAGGGTACACCACACTCTGCACAATTCTGTAGAACC2080.1427512559365306No Hit
TATTTATACGAACTGGAAAGATGCTGCTACCGTATGGATTTGACGGCTCC2030.1393197353611332No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT2000.13726082301589482No Hit
CCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG2000.13726082301589482No Hit
TGTGCAGTAAAGAATGGCCAGTTACGGTAGCAGAGACATGATACTTCCTC1940.13314299832541795No Hit
TCGCTTTTACTGTTCAAGGGTGTGTAGAACCATGTCGTCAGTGTAGATCT1930.13245669421033848No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA1920.131770390095259No Hit
GAAAAATAACATTCTGTGAACTACTTACGACAGGTGTGAAGAAAATGGTG1730.118730611908749No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT1660.1139264831031927No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCCTAATCCAT1640.11255387487303374No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT1640.11255387487303374No Hit
CTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA1640.11255387487303374No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG1540.105690833722239No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC1540.105690833722239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)