FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442639
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT124192.805672342473212No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG111012.507912768644426No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC90132.0361965393921455No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT81901.8502662440498918No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA79631.7989829183601085No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC75291.7009346216668664No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG73301.6559769925379373No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA65331.475920558287905No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC65291.4750168873506402No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT61211.3828424517496198No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA60791.3733539069083385No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA60221.3604765960523135No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT55161.246162222488303No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG54871.2396106081931324No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT52191.179064655396384No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA51311.159183894776556No Hit
TGAAGAAGGAGATGGTGTGGAATTGATGGAAGCCAAATCACAGTTATTTC50341.1372698745478822No Hit
AGTCTTCAGAATGCCAGAGATATACAGGATATGCGAATTAAGAAGAAACA49981.129136836112498No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA49561.1196482912712165No Hit
TTTTGTGCGAAGGAAACCGATGGGGATCGGAACCGTAGCGGTTGAGCTGC49231.112193006038781No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC48461.0947973404964317No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG48141.0875679729983123No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT48131.087342055263996No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC47211.0665576237069034No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC46881.0591023384744678No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA46661.054132148319511No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG45791.0344773054339993No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT45641.031088539419256No Hit
TGCCAAAATGACGAACACAAAGGAATGCCGACAGGGGTACTGTTCAAAAA42450.9590207821723798No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC42360.9569875225635337No Hit
CTAATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTAT38170.862327991885035No Hit
AACTGAGCGACCTCAGAGCCCTGGCAGCTCCAAGGCGCCTTTGCCAGCAG38170.862327991885035No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT36940.8345401105641392No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGACAGTC36820.8318290977523445No Hit
CACATCCTCCTAAACACACAGGCTTTCTCAGAATGTTAGTACTGTTTGAA34700.7839345380773046No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC34300.7748978287046555No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC34190.772412733627177No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT33720.7617946001143143No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA33150.7489172892582895No Hit
TGAACTTATATATTTGACACAACCCAGCAGTTCGTAAGTAGTTCACAGAA32290.7294883641070941No Hit
GCAGAACATTTTGTTTCCTCACTGACGACATGGTTCTACAGCAGCATCTT31360.7084780148156851No Hit
ATTTGTTGCAGGTTTTGGTTTTAATGGCTTGTGGCGGTTTGCAGGACCGT30870.69740804583419No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT30760.6949229507567114No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA29330.6626167147494911No Hit
CGGCAGTTGTGTCTAGAGAATGGCGGGTTCTGCACCTCCGCGGGCGCCCT28430.6422841186610307No Hit
AGATGATACTGCTGCCCTCGGACTGCCGAGGACACAAAGCCAGGCCTAAA27810.6282772191334248No Hit
GATTCCCGCGCCCAGAGTCCCACGACCCCCTCAGACCAAGTCTCTGCTAC27550.6224033580412028No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT26170.5912267107055637No Hit
CCAGAGACCAGACTCCAGAGCTGGGAGGCGAGGTCACATTCCACTGGCCC25320.5720237032886845No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG25170.5686349372739411No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG24440.5521429426688566No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG23670.5347472771265072No Hit
GTGTTTGTTCCAATACAGCAGATGAAATATTACCTAGATCTTGCCTTGGC23260.5254846500195418No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA22830.5157701874439442No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC22650.5117036682262521No Hit
TCAGAAGCTGACATTTAAGGGAAATAAAGAGCTCGCAATAACTAGAAATC22060.4983745219015947No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA21670.4895637302632619No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT21570.48730455292009967No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGCA20960.47352357112680993No Hit
ATTACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGACCTGGTGTC20370.46019442480215256No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA20150.4552242346471956No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT19960.4509317976951872No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTACCTTCAGAGCTAGGGAAAAA19830.4479948671490763No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC19740.4459616075402303No Hit
CCTCAGGGCCTCAGAGAAAGGCAAAAGGGCCTAGACTTTGACTTCCTTTT19500.4405395819166409No Hit
AGAAGACCAGGAAAGAACGGGAGCTGAAGTCTGGGCACAAAGACCGGAGT19410.43850632230779485No Hit
GACTACAGATGTGCCACCATACCCAGCTAATTTTTTAATTTTTTGTAGAG19300.43602122723031633No Hit
GCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAA19290.4357953094960001No Hit
TCTACCCACTCTCTTTTCAGTGCTGCCCATAGACCAAGGCAGAACTGTCA19200.43376204988715406No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT18890.4267586001233511No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA18590.4199810680938643No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGTCTTTGTATGCTGGCT18540.4188514794222832No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC17820.40258540255151487No Hit
CGAAGGGAGAACGACGAACGTCGTGGATCAGATCGGAGCGACCGCCTGCA17690.399648472005404No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG17470.394678281850447No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT17430.39377461091318205No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT17330.39151543357001983No Hit
GCTGGCTTGGGATGGACTTTTTAAAAAGACACTCAGGTTTGGAATGTGGA16770.3788640404483111No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA16710.3775085340424138No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATA15970.36079062170301307No Hit
GACCTTTTCCTCTGCCCTTATCATCGCTTTTCAGTGATGGAGGAAATGTT15960.36056470396869683No Hit
CCAGCTCACAAGAGAAGAAAATACTGCTATACGTACTCCAGAACATTTAA15940.36011286850006435No Hit
ACCAAAGAAGTCCACGAATGGTTCCTGATGGAATCAACCCAAAATTACAC15460.3492688172528855No Hit
CTGTGGTGCCACCTAAGCTCTGCTACCGTATTCTGATGAGCCAAGTCTCT15370.34723555764403946No Hit
AACAATCGACAGGCTAGAAGTTGGCAAAAGTGGTTCACAATGATCTGATG15370.34723555764403946No Hit
TGTTTTTGTAGGTAATTTTAACCAATCAGATGACAACAAAGATTGATAGA15320.3461059689724584No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGCAGCTG14950.337747012802758No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC14890.3363915063968606No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA14610.3300658098360063No Hit
AGCTGACAGAGACAAAGATGAAGGAAAAACAAATCACTCCTTGGGAATTA14490.3273547970242116No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA14440.32622520835263047No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC14390.32509561968104933No Hit
GCTCCAAACAAGATGATAGCCCCTCGGGAGCTTCTTATGGCCAAGATTAT13320.30092242210921316No Hit
CTTTCTAGTTGATACGGCCTTCACTATGTAAAGGTCAGTCTTTTTATTTC13320.30092242210921316No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC13100.2959522319542562No Hit
GACAGCAGAACACACATACCAAAGTCAGTACTGAGCACAACAAGGAATGT13040.2945967255483588No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC12760.2882710289875045No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC12600.28465634523844485No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC12410.2803639082864366No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGC12080.2729086230540011No Hit
TCCACAAACAGAACAAGATGCTCCTAGGCACAACTTCAAGGCAATCTCAA11810.266808844227463No Hit
CTGTTCAAGAGTCAAGAGATTAGATTTTGTACACTGACGCCATGGTTCTA11510.2600313121979762No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA11500.25980539446366No Hit
GGGATAATGGGGTTTTCCTGTGTCAGAATCTCAATGGAACCCAGCATCCT10500.2372136210320374No Hit
GGTATTTAAAGGCTTTTGTTTTCTGTTGGGGTTTTTATAGAACCTGCTTT10130.22885466486233702No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT9950.22478814564464494No Hit
TGGTAAATTCATCTGCTTTCTCTGGATTTAGTACAGCAAGTGGAAAGCAA9770.22072162642695287No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG9580.2164291894749446No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGCAG9340.21100716385135518No Hit
ACCTTATACTCAATTCTCAACTCCTTGTTTTTAGGTGGTTAGCCAGCGTT9240.2087479865081929No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC8700.1965484288551167No Hit
CACCTCGAACACCCAGGCTGGAGTGAAGTGGCACTATTTTGCCTCACTGC8670.195870675652168No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGCAGCT8300.18751171948246764No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA8040.1816378583902458No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAAAGGCACCTGATATATGT7750.17508624409507523No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACGCGATATATGT7550.1705678894087507No Hit
TTTCTTACCCTGTGCAGAGCATTGGTCTTTGCTTCATCTTCCACAAAAGA7490.16921238300285332No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC6920.15633507214682846No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC6790.15339814160071752No Hit
GTCTCGGAGGCTGGCATCTTCTGCTTCTGTTTCAAAAGACCAAGTCTCTG6580.14865386918007675No Hit
TCAAATGTAGCAAATCAGAAGCCCTTTGAGAGTGGAAGTGACAAAATCTC6570.14842795144576054No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGCA6560.1482020337114443No Hit
GAGATTGATGACCAAAAGAACTGCACACTGACGACATGGTTCTCCTCAGA6530.14752428050849561No Hit
TAAAGGAACTGTAGTCGCCCTGCTTGAGCCTGGACATGGGGAGACCAAGT6410.14481326769670094No Hit
CTTCTTCAGGTCAGGGGAAAGTACGGTAGCAAAGACATGGGGTTGGTGAC6360.1436836790251198No Hit
GGTGCCAGTCTTGCTCACAGCAGTTTGCTCACAGAAGGAGGACTCAGACC5770.13035453270046243No Hit
GTAACCAGGCATTATGATCTTTGAAAATTTTTATTTGCGTATCTTTAATT5760.13012861496614622No Hit
CTCCTAATTGTGAGATATATTATCAAAGTCCTTTATCACTTTGTATGGCC5750.12990269723183No Hit
AGTCTTCTCCTAAACCATAGTCTGCCTTTTGATAGCTCTAATAATTAATT5740.12967677949751377No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT5670.12809535535730018No Hit
ATGAGCAAGTTGGGGTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGC5670.12809535535730018No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC5670.12809535535730018No Hit
TTGTTCAAGAGTCAAGAGATTAGATTTTGTACACTGACGCCATGGTTCTA5560.1256102602798217No Hit
TGGATCCTTTATTACGAATTGTGATCACATCCTCTGATTGGCAACATGTT5410.1222214942650783No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT5400.12199557653076208No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA5220.11792905731307002No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG5190.11725130411012134No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG5060.1143143735640104No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCACCTA5040.11386253809537794No Hit
CCGACTACCTCAGGATGGACACAAGTATGATCCCTGCTGGAAAGAATAGT5010.11318478489242927No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT4880.11024785434631834No Hit
GCAGCATCTTGAATCTCATACAGACCAAGTCTCTGCTACCGTAGCAGCTG4810.10866643020610474No Hit
CTTGAGGCATTGTTATTTCAGCATTCTTTTTTTTTTCTTGCTTTTAATTT4760.10753684153452363No Hit
TGGTGATAGATGACTTGCTGCTACCGTAGTGGGGTTCGCTCATTATTTCC4740.10708500606589116No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAAAGA4720.1066331705972587No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGCAGC4640.10482582872272891No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGCAGCTGTCAATC4600.10392215778546401No Hit
GGGCCCCTTCCGCTTTACACACTGACGACATGGTTCTACAGATTCCCGCG4490.10143706270798551No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)