FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008098970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008098970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442639
Sequences flagged as poor quality0
Sequence length150
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT113232.558066505662628No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT110602.4986501415374605No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG101782.2993906998705493No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT99202.241103924416963No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT78631.7763911449284857No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG75311.7013864571354989No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG71931.6250262629366143No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA65411.4777279001624348No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA60341.3631876088641082No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA59341.3405958354324856No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT58321.3175522265322306No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC51941.1734167120384784No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA51251.1578283883706586No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA50001.1295886715811303No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC48391.093215916356218No Hit
TATGAGAACACGCAGAGGGAACTTGGCCTCCTACTGCTGCTTTCAGAGAG47771.079209016828612No Hit
CTGAGGTCGCTCAGTTCCGGAGCTGCAACCCGGAGTCCCAGATGATCGGG47231.067009459175536No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT46511.0507433823047676No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG46341.0469027808213915No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT45621.0306367039506235No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA45381.025214678327034No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA45181.0206963236407094No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT44200.9985563856777193No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG44120.9967490438031894No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC43290.9779978718549428No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG42420.958343028969431No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA41680.9416251166300303No Hit
GGCATTCCTTTTTGAACAGTACCCCTGTCGGCATTCCTTTGTGTTCGTCA41290.9328143249916975No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT39020.8815309993019141No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA38790.8763348914126411No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG38620.8724942899292651No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCACTGCTGCT37000.8358956169700366No Hit
CACTGGGCAATTGGTAGGGTAAATCCACAGGGCGCCCGCGGAGGTGCAGA36920.8340882750955068No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC36650.8279884962689686No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT34170.7719608981585445No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA33790.763376024254528No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT33520.7572762454279899No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT32710.7389769089483755No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG31970.7222589966089747No Hit
TATGAAGTAGCCTGCTATAATCGATCAAACTAACCCTAACTATCAAGATG31920.7211294079373937No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC31920.7211294079373937No Hit
GTATGAGATTCAAGATGCTGCTGTAGAACCATGTCGTCAGTGAGGAAACA31470.7109631098931636No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTTCAGATCTTCAGGGGGCTAGAA30310.6847566527124813No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC30200.6822715576350028No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC28490.6436396250669281No Hit
GAGGGGGTCGTGGGACTCTGGGCGCGGGAATCTGTAGAACCATGTCGTCA28060.6339251624913304No Hit
GACGGCTGCTCTTTTCGTTCTGTCGCCTGCCCGATGGACGAGCCTCCCGG26520.5991338314066316No Hit
ACCAGCGGTAGAAATTGTACATGACCACGGCGGCAATAAAGGTCTTTCCC26220.5923562993771448No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT25780.5824159190672309No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAACAGAAACCTTCATTT24420.5516911072002241No Hit
GACAGGTTCATGAGTGCTTCCCCCACATTCCTTCAGGTGACTGGTTCTTC24420.5516911072002241No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC23970.541524809155994No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG23540.5318103465803963No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT22770.5144146810380468No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA22610.5107999972889872No Hit
ACATGCACAGTTGCTCTGGGAGTCTTCAGAATAGAAACTACCCATCTCAA22440.5069593958056113No Hit
CCCTCTCTGCTTCTCCTCCTCCTGCAGGCTTGGCCTTGATGATGCAGCTG21100.476686419407237No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA21070.47600866620428833No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT20280.4581611651933065No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA19820.44776894941476014No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA19580.4423469237911707No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC19510.44076549965095707No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT19140.43240654348125673No Hit
CTTGGCCATAAGAAGCTCCCGAGGGGCTATCATCTTGTTTGGAGCTGTCA19120.43195470801262426No Hit
AGGGCCTATTAGCCATATCTCCCAGCTCTCAACAGGAGGTGGAGAGGCAG18780.4242735050458726No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA18620.420658821296813No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA18580.41975515035954813No Hit
CTGGGTATGGTGGCACATCTGTAGTCCTAGCTGCTCAGGAGGCTGAGGTG18520.41839964395365076No Hit
TCATTGGAATGGCAAAGTCTCATACTTGTAATCCTAGCGCTTTGGGAGGC18520.41839964395365076No Hit
TGGTCATTTGACAGTTCTGCCTTGGTCTATGGGCAGCACTGAAAAGAGAG18110.40913701684668546No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT17550.39648562372497675No Hit
ACTTTTTCCCTAGCTCTGAAGGTACGGTAGCAGACCTTTGTGCTTTTATC17470.394678281850447No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG17440.39400052864749835No Hit
GAACGCTTTGAACTTCCCGATATCCGGTCCTTCATCGATCCCGACTTGCT17400.3930968577102334No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT17090.3860934079464304No Hit
GGAAAAACCAAGTGTGGGAGGCCCCAGTCCTCTCTGCCAATCCGCTATAC16730.3779603695110463No Hit
TAATGATCAAGGGGAGAAGGTGCAGTGTAGAACCATGGCGTCAGTGTACA16580.37457160349630286No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC15950.3603387862343806No Hit
AGAGACATCTTAAAGAGGGAAGCTGTATTTTTCCAGAGGAACTGAGTCCT15430.34859106404993684No Hit
CACAGAGACTTGGCTCATCAGAATACGGTAGCAGAGCTTAGGTGGCACCA15080.3406839433488689No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC15060.3402321078802365No Hit
ACTTGCTTTCCACTTGCTGTACTAAATCCAGAGAAAGCAGATGAATTTAC14870.3359396709282282No Hit
AGAAAGAAATATATGGTAAGTTTCAAGAATACATCATACCTGTATAGGGT14780.33390641131938215No Hit
CGACCTGCTAAGTAAGAACAGGGGAAAAATGCATGTGTCAAATGCAAAAT14770.3336804935850659No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA14420.325773372883998No Hit
AATCAAATGAAAGAGATAAGAAAAACTGTGATAAACTAAAATGTTTATCT14330.323740113275152No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC14140.3194476763231437No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA14000.3162848280427165No Hit
ACCTCTCAGAAACTTCTCTCATTAACTAAAGTCAGCTCTCCCGCTGGGCC13710.309733213747546No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC13390.3025038462494267No Hit
CCCCAAAACACAGTAACCCAAATACTCACCATATTGTTAACATTCTTTAA13320.30092242210921316No Hit
AGCATAATTTTTGGCTGCTTTGTTTTATTTAGGTTCCAGTATTACAGATA13070.29527447875130747No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG12930.29211163047088035No Hit
TACTGTAGGGGCTCGGTGACCGGGTGCTGGACTGGTAGGCCGAAGGCTCC12840.2900783708620343No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG12800.2891746999247694No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG12540.28330083883254753No Hit
TGCAAGGACCGTACAGTGCCTCAGTGATGCCAGCCTCCGAGACATGCCAC12180.2751678003971634No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCTTTGCCGTC11970.27042352797652264No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG11940.269745774773574No Hit
TGAGATTGCCTTGAAGTTGTGCCTAGGAGCATCTTGTTCTGTTTGTGGAT11690.2640978314156683No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT11510.2600313121979762No Hit
GTCTCTGTATGGCAGCAAATGTGGCCCATGGCCTGCAGCAAAACGTCCTA10300.2326952663457129No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA9620.21733286041220948No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA9490.21439592986609857No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT9460.21371817666314988No Hit
CACAGTGCTCTGGGTTTCTCTTATCAACACGAGGAAGTATTTTTGATACA9400.21236267025725253No Hit
CACACACAGGTAACGGCTGAGGCAGGAGAATCGCTTGAACCGGGGAGGCG8780.19835577072964652No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG8770.19812985299533029No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT8370.18909314362268123No Hit
ACCTTTGACATTAACTTCAAGCCCCTTTCGATTCTAGGTGGTGGCTTTTT8340.18841539041973257No Hit
CTGCCCCCTTACCCTCCACACCCCCAAGATTCTTACAGAAAACAAGTGGT8260.18660804854520274No Hit
TATGGTCCTCAGAAAAGTGCACCAAACCAGATTCTAGCAGTCCCATTAAG7890.1782490923755024No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA7700.1739566554234941No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTGTAAAGCGGAAGG7670.1732789022205454No Hit
TTTGTGGAAGATGAAGCAAAGACCAATGCTCTGCACAGGGTAAGAAATGT7480.1689864652685371No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC6920.15633507214682846No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG6840.15452773027229866No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT6820.15407589480366618No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTATAATGTCACC6710.1515907997261877No Hit
TTTGAAACAGAAGCAGAAGATGCCAGCCTCCGAGACTGTAGAACCATGTC6530.14752428050849561No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA6510.1470724450398632No Hit
CCCCATGTCCAGGCTCAAGCAGGGCGACTACAGTTCCTTTATGTAGAACC6380.14413551449375225No Hit
GTCACCAACCCCATGTCTTTGCTACCGTACTTTCCCCTGACCTGAAGAAG6380.14413551449375225No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTATTTTCTCCATCTGGGCTC6140.13871348887016283No Hit
TTAAATGGAGTCATCTGAGGAGAACCATGTCGTCAGTGTGCAGTTCTTTT6090.1375839001985817No Hit
AAACCAATGCTCTCACCTTAAAGTATTGGTTGTTGTGAGGGCTTATACGA5920.13374329871520585No Hit
GAGTCCTCCTTCTGTGAGCAAACTGCTGTGAGCAAGACTGGCACCTGTAG5850.13216187457499226No Hit
CAGACAGGGACAAATCATCAGCTGGAGACACCAGGTCTGCCTGAGAAGCC5770.13035453270046243No Hit
ACAAGTCAACAACCCCCACACCCCAACTTGCTCATTGTAGAACCATGTCG5680.12832127309161642No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT5600.12651393121708662No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA5520.1247065893425568No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT5390.12176965879644587No Hit
AGTGACGTGTCCTCTGTTCCTTCTCTTTCTTCACAGGTTTTGAAGGAAGA5260.11883272825033492No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG5090.11499212676695908No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT5040.11386253809537794No Hit
CAGTCAAGAGTCATCGAATACCATTAAGAAGGATCATACAGGGAAATCCA4880.11024785434631834No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG4870.1100219366120021No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT4860.10979601887768588No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT4850.10957010114336965No Hit
GTATGAGATTCAAGATGCTGCTGTAGAACCATGTCGTCAGTGTAGATCTC4790.1082145947374723No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAGCAGAAACCTTCATTT4690.10595541739431003No Hit
AGAAATCCAAGGCTCTTCTCTTTTTGCAGTTCTTTTGGTCATCAATCTCT4680.1057294996599938No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC4630.10459991098841269No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT4630.10459991098841269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)