FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352492
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT163884.64918352756942No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC142984.056262269782009No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA138013.915266162068926No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA129283.6676009668304532No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG128603.6483097488737335No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT82842.350124258139192No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC79392.2522496964470116No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA77962.211681399861557No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA77132.1881347661790906No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA71592.030968078708169No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT68281.9370652383600195No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC67391.9118164383872542No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT66961.8996175799734463No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA61551.746138919464839No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG57651.6354981105954176No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA49801.4127980209479931No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT49201.3957763580450053No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT47471.3466972300080569No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA45881.301589823315139No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC45621.294213769390511No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC44731.2689649694177456No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC40241.1415861920270531No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG39541.1217275853069006No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA36231.0278247449587508No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA35040.9940651135344916No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC34940.9912281697173269No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA33970.9637098146908298No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC33930.962575037163964No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC32950.9347729877557505No Hit
CTTTTTCTGGTTGGGCAGTTGGGCTATAGCAGCTTTGTGGAGCCATTGTC32710.9279643225945553No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA32260.9151980754173145No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG29760.8442744799881984No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG29320.8317919271926738No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG25390.7203000351781034No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT24890.7061153160922801No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC24460.6939164576784721No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA24350.690795819479591No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT24110.6839871543183959No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA22440.6366101925717463No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG21930.6221417791042066No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC20570.5835593431907674No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG19950.5659702915243467No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT19700.5588779319814351No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG18780.5327780488635203No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG18430.5228487455034441No Hit
ACACAGATTCTGAAATGAGAGCTACCGTATGGATGATGATGTTGAAATAT18150.514905302815383No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC18070.5126357477616513No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG18010.5109335814713525No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG17440.494763001713514No Hit
CTAATGAGCGGACGGCAAAGAACGCTTTTTCAGACGTGGGGCTCAAGTAT17270.4899401972243342No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT16740.47490439499336157No Hit
GGGATAATGGGGTTTTCCTGTGTATGCATTCTGAAAAAGCCAGAATTTAC16450.4666772579235841No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC16180.4590175096172396No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC14890.4224209343758156No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT14890.4224209343758156No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT14080.399441689456782No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA13790.39121455238700453No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA13360.37901569397319657No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA13320.3778809164463307No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA12460.35348319961871477No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG12290.34866039512953484No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG11370.3225605120116201No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAATC11090.3146170693235591No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT10890.30894318168922985No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT10830.307241015398931No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT10810.30667362663549813No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA10800.30638993225378164No Hit
ATTACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGACCTGGTGTC10700.303552988436617No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT10500.29787910080228774No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT9880.2802900491358669No Hit
AACTGAGCGACCTCAGAGCCCTGGCAGCTCCAAGGCGCCTTTGCCAGCAG8790.24936736152877229No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG8180.23206200424406798No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT8130.23064353233548562No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA7990.22667181099145514No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA7850.22270008964742463No Hit
AGATGAGTCATATTTGTGGGTTTTCATACAGCTTCCCTCTTTAAGATGTC7540.21390556381421424No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC7090.20113931663697332No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC7070.20057192787354042No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA7050.20000453911010746No Hit
GTTAGAAGGCTGGCTCCCATGCTGTTCTAACACAGCTTCTAGTTCAGCCA6910.19603281776607698No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAATCATAT6870.19489804023921112No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG6820.1934795683306288No Hit
CGATGCGCGGGAAGACGTTCCGCTTTGAAATGCAGCGGGATTTGGTGAGT6780.19234479080376293No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAT6730.1909263188951806No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA6360.18042962677167143No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC6350.18014593238995497No Hit
CCAGAGACCAGACTCCAGAGCTGGGAGGCGAGGTCACATTCCACTGGCCC5420.15376235489032375No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA5150.14610260658397922No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA5130.1455352178205463No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC4950.14042871894964992No Hit
GCCCTCTCAGAAACATCTGCAGGAGGACTTGGGGAAACAAATTCATATGA4870.1381591638959182No Hit
GCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGACAGAG4650.131917887498156No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAATCATA4640.13163419311643954No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT4410.12510922233696084No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTAAGGTTTCAATGAATG4280.12142119537464681No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT4170.11830055717576568No Hit
GCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAA4140.1174494740306163No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAATCA3970.11262666954143641No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC3880.11007342010598822No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAA3750.10638539314367418No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC3690.1046832268533754No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT3620.10269736618136013No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCATCTTACCATGGAAGCCAT3590.10184628303621075No Hit
AGTCTTCTCCTAAACCATAGTCTGCTACCGTATACAGCAGAAGAACGTGC3540.10042781112762844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)