FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285937
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC65882.304004028859504No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA64232.246299009921766No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC64052.24000391694674No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA58342.0403095786834164No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT54021.8892273472827932No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC53651.8762874339452396No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA52791.8462108786201157No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT52361.8311726009575535No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC48011.679041187394426No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG44571.5587349660939298No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC44371.551740418343901No Hit
GGAAACACTGATGGGCCTGAAAGTCACACCACCTCAGCAACCAGACCAAG43701.5283086833813042No Hit
GACTACAGATGTGCCACCATACCAACTGCTCTGGAACATGTTAGACCAAG43251.5125709509437395No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA42661.4919370350811543No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC40101.402406823880785No Hit
GTTGCCTTATTAACGGTATCTTCAAAACTAGTATCTTCCTTTATTTCACC37741.3198711604304445No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA36501.2765049643802655No Hit
AGTGGAACTGTTATTATCCCATCAAATGGCACAGCTGAGACCAAGTCTCT35391.2376852243676053No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT33551.17333538506734No Hit
GAAAGTGGTGCATTGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTCT31821.1128325470295903No Hit
AAGTCCTCCATTTCTGTATCCATGGAGGAAGTAGGCAGCTGAGACCAAGT31201.091149449004501No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC29941.0470837981793193No Hit
TCTCCCCCACCCCCTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT29681.0379908861042817No Hit
GAAAGTGGTGCATTGATGGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC28881.0100126951041664No Hit
GCTTAGATTCCTCTTTGGAGTCAGTGTGCAAGTCATGGTAAGTCCTCTGT28751.0054662390666476No Hit
TCAAAGGGCTCCACTGGTTTTTCTTATCTCTTTCATTTGATTAGACCAAG28010.9795864123915409No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATGTTGTG26940.9421655819288866No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC26550.92852621381633No Hit
AGCAATCTGCTTGTGTGTGCGTTCTGATGAGCCAAGTCTCTGTGAGACCA26300.9197830291287941No Hit
ACATGTACATAGGGTTTATATATCATCAGCTATAAGTATCTTCTCTTGTC25270.8837611082161455No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA24520.8575315541535374No Hit
GCCTACCAGTCCAGCACCAGAACGAAAAGAGCAGCCGTCAGACCAAGTCT24320.8505370064035085No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG24040.840744639553468No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATGT23220.8120669937783497No Hit
GGGGTCAGAGGCAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGT23140.8092691746783383No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATG22880.8001762626033008No Hit
GGTGCCAGTTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACCG22390.7830396206157301No Hit
CACATCCTCCTAAACACACAGATGACGATTCCTTCTGTGTTTAGACCAAG21940.7673018881781651No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC21840.7638046143031507No Hit
TTGGACTCAATTTCAACTCTAACTTTAAATGCAAACTAGGCCAGGCAGAC20740.725334601677992No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA19520.682667860402816No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATGTT18820.658186943277715No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC17820.6232142045275707No Hit
GTAACTTGATTTGCTGTTGTATTTGCTACCGTAACCCTCAATTTGGAAGC17440.6099245638025159No Hit
CCTTCTTCCGATAGGTTTTCCCAGCGTAGGCAACCACTGACTCTGATCCA16870.5899901027149337No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC16850.5892906479399308No Hit
TCAAAGGGCTCCACAGTTTTTCTTATCTCTTTCATTTGATTAGACCAAGT16590.5801977358648933No Hit
AACTGATCACCTTGAATCTGCTTGGGAGAAGGAATATTTGATGGTCAACC16200.566558367752337No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT16130.564110276039827No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA15740.5504709079272707No Hit
GGGGTCAGAGGAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGTA15500.5420774506272361No Hit
GTTGTATCCGCTGCTTTGTCCTGTTGTGTAAGTATCTCCTTTTGAAGACC15210.5319353563896942No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC14880.5203943526021466No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC14430.5046566201645817No Hit
GGCTTCCACTTAATTCCAGTCTAAAAAGTTACCCAGAAGTGTAGACCAAG13890.4857713412395038No Hit
ATTATCCAGATGAATTTACAAAACTGTACATGTGCTCTTGAGTGAGACCA13740.48052543042698215No Hit
AGTTCCATTTAGGTTGCTGGTGAAGAACATTCAGGCAAGACCAAGTCTCT13680.4784270661019735No Hit
CATCTCCATGAGTAGGAAGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC13430.46968388141443745No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT13400.46863469925193313No Hit
GCCTGCTGAAAATGCCAGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA12940.4525472394268667No Hit
GTTGTTTCAGAACACCGAAAGCTCAAGAAGCATGTCATAGACCAAGTCTC12900.451148329876861No Hit
TCCTCTCTTAGATTTGGCTTTCTGTTACCGTAACCACTGACTCTGATCCA12680.4434543273518292No Hit
TCTCCCCCACCCCTTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT12610.4410062356393191No Hit
TGGGGAAAAATATGCTTTGAGCTACAGAAAAACATAGAAATCAGAGACCA12140.4245690484267513No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC12080.42247068410174266No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC11940.41757450067672247No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT11650.40743240643918066No Hit
TGTGCTGTGACTGCTTGTAGAACACAGAGTTGAAAGTCAGGGCAAGACCA11580.40498431472667057No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA11550.4039351325641662No Hit
GCCTGCTGAAAATGACTGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA11550.4039351325641662No Hit
GTTGTTTCAGAACACCGAAAAGCTCAAGAAGCATGTCATAGACCAAGTCT11530.40323567778916336No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATGTTGT11150.3899460370641085No Hit
TGGGGAAAAATATGCTTTGATCTACAGAAAAACATAGAAATCAGAGACCA11070.387148217964097No Hit
CCTCAGGGCCTCAGAGAAAGGACTTCACCTTCTCTCCTCCCTACTTAAGA10780.3770061237265551No Hit
CCAAATCTGCTTTCTTGATAAAATCCTCTGAATGGCATGCAGACCAAGTC10600.37071103075152917No Hit
TCTAAGCATAGCATTCAATTTTGGCTACCGTATGTTATTACGGCTAATTG9970.3486782053389383No Hit
GCTTTCACTTCTCTGGCTGAACACACAGCTCAGAATACTAGTAGACCAAG9740.3406344754264051No Hit
CACATCCTCCTAAACACACAGGTGACGATTCCTTCTGTGTTTAGACCAAG9390.3283940168638546No Hit
TTAGTTGCTTTTGAATTTACAGTTTAGTGAAAAAAGTCTTTATAGGTTAG9150.32000055956382No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG9080.31755246785130986No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATGTTGTGGTAATC8590.3004158258637392No Hit
AGTTCCATTTAGGTTGCTGGTGGAGAACATTCAGGCAAGACCAAGTCTCT8260.28887482207619164No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATGT8250.28852509468869014No Hit
CATCTCCATGAGTAGGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC8160.28537754820117717No Hit
CAAAGTGGGAAGGGGAGTGAACATCGAATCATGGAATCCCAGACCAAGTC8130.28432836603867284No Hit
CAAAGTGGGAAGGGGAGTGAATATCGAATCATGGAATCCCAGACCAAGTC8030.2808310921636584No Hit
GTACAATTTCTACCGCTGGTTCCACATGAAAAAGAGCAAGGTACTAGTGA7900.2762846361261397No Hit
ACGAAAAAGTAACCTTGATGAAGAGGTACGGTAGAGGAGCTTGTCCCGCT7900.2762846361261397No Hit
CTACTAATGCCCACAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT7100.2483064451260243No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA7100.2483064451260243No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT7060.2469075355760185No Hit
TGCATTCTGAAAAAGCCAGAATTTACAGCTCTCCAGACCAAGTCTCTGCT6990.2444594438635084No Hit
CACATCCTCCTAAACACACAGGAGGAGGAGGAGGAGGAGGAAGAGAGAGG6910.24166162476349684No Hit
GGATACAGTAGCCAGAATGACGACATGGTTCTACAGTGGGGTTCGCTCAT6870.24026271521349107No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT6850.2395632604384882No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACCAACTG6780.2371151687259781No Hit
GTTAACTGCTACTAAAACGGAGCACCACTTACATTTGCAAGACCAAGTCT5910.20668888601335259No Hit
AAGAAGAACAAACAACAGGACCAGCTAATGAAGTCCAGTCTACCACACCA5890.20598943123834973No Hit
GTTAACTGCTACTAAAACGAAGCACCACTTACATTTGCAAGACCAAGTCT5760.20144297520083096No Hit
CTACTAATGCCCACAAAGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT5690.19899488348832087No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG5600.19584733700080786No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAAAGA5450.19060142618828624No Hit
ATTATCCAGATGAATTTACAAAACTGTACCTGTGCTCTTGAGTGAGACCA5360.18745387970077323No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA5170.18080905933824584No Hit
CACTTAATGAGACCAACAGTAACACCTTTATCACTCACAAGACCAAGTCT5140.1797598771757415No Hit
TCTGGGGTATCAGGTAGGTGTCTACGGTAGCAGAAAGTCAGTCCCATGGA5080.17766151285073284No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCAGTCAAATGTCCATGGATCAT5060.17696205807572996No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC4940.17276532942571265No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG4880.170666965100704No Hit
GCGTGGACAACCCCCACGGGAGGCAGGACAGGGGCCAGGAGGAGACCAAG4860.16996751032570112No Hit
AGTAAGGGTGGCATCAAAAATTGGTTTTCTTTGCAGACCAAGTCTCTGCT4860.16996751032570112No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATGTTG4850.16961778293819968No Hit
GGCTTCCACTTAATTCCAGTCTGAAAAGTTACCCAGAAGTGTAGACCAAG4370.15283086833813045No Hit
TGCATTCTGAAAAAGCCAGACTACGGTAGAGGAGCTTGTCCCGCTAGACC4350.15213141356312754No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA4260.14898386707561456No Hit
GCCACACCTCACGTTAGAATTTTCCAGTGAAGACCAAGTCTCTGCTACCG4190.14653577536310444No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC4170.14583632058810156No Hit
CTGGCCATTCTTTACTGCACAAGACCAAGTCTCTGCTACCGTATGTTGTG4040.14128986455058282No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCAAGGTAATT3960.13849204545057128No Hit
GATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTATGTTGTGGT3790.13254667986304675No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTATG3770.13184722508804386No Hit
GCTTTCACTTCTCTGGCTGAGCACACAGCTCAGAATACTAGTAGACCAAG3650.12765049643802656No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT3590.1255521321130179No Hit
TGCTAATTGGGCAGATAGAGCTTCGTTCAGACCAAGTCTCTGCTACCGTA3560.12450294995051357No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATGTTG3460.12100567607549916No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG3450.12065594868799771No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTATGTTGT3420.11960676652549337No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTAAAATGAAATACTTTGAGAA3410.11925703913799193No Hit
GCCCTCTCAGAAACATCTGCTACCGTACTCATGGTGGGGGCAGCAGACCA3400.1189073117504905No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTATG3360.11750840220048472No Hit
GTGCACATTCAAAAAAGAAATTATCCAGATTTCCTTTCAGAACACTCAGA3340.11680894742548184No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATGTT3310.11575976526297752No Hit
ATACCACTGACGGCCAGGTAGAGATTTTATTTTGTTTTTCAGAGACCAAG3290.11506031048797462No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACGACATGGTTCTACAGGGGA3150.11016412706295443No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCAAAAT3100.10841549012544723No Hit
GAATTGTGCAGAGTGTGGTGTACCCTTTCGGTAAGACATGTAGACCAAGT2930.1024701245379227No Hit
AAGTGGTTCTGGATTAGCTGGATTTCAGTGCGCTTTTCCCAACACCAACT2880.10072148760041548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)