FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285937
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG66062.31029912183453No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG65272.282670658221916No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA63952.2365066430717255No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT63532.221818092796665No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT53571.8734896148452282No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC52061.8206807793325102No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT52051.8203310519450087No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC49661.736746206332164No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA47821.6723963670318986No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG44491.5559371469939183No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC44351.551040963568898No Hit
GGTTGCTGAGGTGGTGTGACTTTCAGGCCCATCAGTGTTTCCTGTAGAAC43621.5255108642812927No Hit
AACATGTTCCAGAGCAGTTGGTATGGTGGCACATCTGTAGTCTGTAGAAC43001.5038277662562032No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT42561.48843976120614No Hit
GGTGAAATAAAGGAAGATACTAGTTTTGAAGATACCGTTAATAAGGCAAC37441.3093793388054011No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA36571.2789530560927755No Hit
CAGCTGTGCCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGT35361.2366360422051013No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT33561.1736851124548415No Hit
GAGACCTACATCAGGCCTTCATCAATGCACCACTTTCTGTAGAACCATGT31841.1135320018045933No Hit
CAGCTGCCTACTTCCTCCATGGATACAGAAATGGAGGACTTTGTAGAACC31171.0901002668419968No Hit
GCAGATGTCTTTGAGTGAGTAGAGGGGGTGGGGGAGATGTAGAACCATGT31141.0890510846794923No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG29961.047783252954322No Hit
GAGACCTACATCAGGCCTTCCATCAATGCACCACTTTCTGTAGAACCATG28861.0093132403291634No Hit
ACAGAGGACTTACCATGACTTGCACACTGACTCCAAAGAGGAATCTAAGC28520.9974225091541145No Hit
AATCAAATGAAAGAGATAAGAAAAACCAGTGGAGCCCTTTGATGTAGAAC27990.978886957616538No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC26910.9411163997663822No Hit
CACAGAGACTTGGCTCATCAGAACGCACACACAAGCAGATTGCTTGTAGA26350.9215316660663013No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA26270.9187338469662899No Hit
CCATGGACAAGAGAAGATACTTATAGCTGATGATATATAAACCCTATGTA25400.8883075642536643No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT24510.8571818267660359No Hit
GACGGCTGCTCTTTTCGTTCTGGTGCTGGACTGGTAGGCTGTAGAACCAT24150.8445916408159839No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC23970.838296547840958No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA23390.8180123593658742No Hit
ACACCGGATATCTGCTTGCCTCTGACCCCTGTAGAACCATGTCGTCAGTG23320.8155642676533643No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTAGAACCATGTCGTCAGT22800.7973784435032891No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG22790.7970287161157878No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG22140.774296435928194No Hit
AAACACAGAAGGAATCGTCATCTGTGTGTTTAGGAGGATGTGTGTAGAAC21890.765553251240658No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG21620.756110611778119No Hit
GCCTGGCCTAGTTTGCATTTAAAGTTAGAGTTGAAATTGAGTCCAATGTA20710.7242854195154876No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT19100.6679793101277554No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT18520.6476951216526717No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA17860.6246131140775766No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTTAGCAGAGACTTGGTCTGTCTAA17350.6067770173150029No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG16920.591738739652441No Hit
AAGAGGCTTCCAAATTGAGGGTTACGGTAGCAAATACAACAGCAAATCAA16700.5840447371274091No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCCTAATCCAT16570.5794982810898904No Hit
AATCAAATGAAAGAGATAAGAAAAACTGTGGAGCCCTTTGATGTAGAACC16480.5763507346023774No Hit
GGTTGACCATCAAATATTCCTTCTCCCAAGCAGATTCAAGGTGATCAGTT16130.564110276039827No Hit
TCTGGATCAGAGTCAGTGGTTGCCTACGCTGGGAAAACCTATCGGAAGAA16080.5623616391023197No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT15880.5553670913522909No Hit
ACACCGGATATCTGCTTCCTCTGACCCCTGTAGAACCATGTCGTCAGTGT15660.5476730888272592No Hit
TCAAAAGGAGATACTTACACAACAGGACAAAGCAGCGGATACAACTGTAG15220.5322850837771956No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG14840.5189954430521408No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA14280.49941070935206006No Hit
ACACTTCTGGGTAACTTTTTAGACTGGAATTAAGTGGAAGCCTGTAGAAC13750.4808751578144836No Hit
CACTCAAGAGCACATGTACAGTTTTGTAAATTCATCTGGATAATTGTAGA13750.4808751578144836No Hit
TGCCTGAATGTTCTTCACCAGCAACCTAAATGGAACTTGTAGAACCATGT13720.4798259756519793No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA13490.4717822457394461No Hit
GAGACCTACATCAGGCCTTCTTCCTACTCATGGAGATGTGTAGAACCATG13430.46968388141443745No Hit
AACATCTGAGGGGTTCTGGCATTTTCAGCAGGCTGTAGAACCATGTCGTC12800.4476510560018466No Hit
ATGACATGCTTCTTGAGCTTTCGGTGTTCTGAAACAACTGTAGAACCATG12690.44380405473933066No Hit
GCAGATGTCTTTGAGTGAGTAGAAGGGGTGGGGGAGATGTAGAACCATGT12510.43750896176430476No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA12140.4245690484267513No Hit
TCTGGATCAGAGTCAGTGGTTACGGTAACAGAAAGCCAAATCTAAGAGAG12060.4217712293267398No Hit
CTGATTTCTATGTTTTTCTGTAGCTCAAAGCATATTTTTCCCCATGTAGA11910.4165253185142182No Hit
TTTGAAACAGAAGCACATTAATATTTTATAAGCCATGTAGAACCATGTCG11810.41302804463920373No Hit
ATGACATGCTTCTTGAGCTTTTCGGTGTTCTGAAACAACTGTAGAACCAT11500.40218649562665904No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA11500.40218649562665904No Hit
TGCCCTGACTTTCAACTCTGTGTTCTACAAGCAGTCACAGCACATGTAGA11480.4014870408516561No Hit
AACATCTGAGGGGTTCAGTCATTTTCAGCAGGCTGTAGAACCATGTCGTC11460.40078758607665327No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT11410.399038949139146No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT11280.39449249310162726No Hit
CTGATTTCTATGTTTTTCTGTAGATCAAAGCATATTTTTCCCCATGTAGA10940.38260176192657824No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG10800.37770557850155806No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTCCTTTCTCTGAGGCCCTGAGGTGT10710.3745580320140451No Hit
GCATGCCATTCAGAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA10580.3700115759765263No Hit
GTGAGCACAATTAGCCGTAATAACATACGGTAGCCAAAATTGAATGCTAT9940.34762902317643396No Hit
ACTAGTATTCTGAGCTGTGTGTTCAGCCAGAGAAGTGAAAGCTGTAGAAC9730.3402847480389037No Hit
AAACACAGAAGGAATCGTCACCTGTGTGTTTAGGAGGATGTGTGTAGAAC9390.3283940168638546No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT8890.31090764748878247No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT8760.3063611914512637No Hit
ACATAATTCTAACCTATAAAGACTTTTTTCACTAAACTGTAAATTCAAAA8580.3000660984762378No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA8400.2937710055012118No Hit
TGCCTGAATGTTCTCCACCAGCAACCTAAATGGAACTTGTAGAACCATGT8260.28887482207619164No Hit
GAGACCTACATCAGGCCTTCATCCTACTCATGGAGATGTGTAGAACCATG8170.28572727558867866No Hit
GGGATTCCATGATTCGATGTTCACTCCCCTTCCCACTTTGTGTAGAACCA8140.2846780934261743No Hit
AGCGGGACAAGCTCCTCTACCGTACCTCTTCATCAAGGTTACTTTTTCGT8020.280481364776157No Hit
GGGATTCCATGATTCGATATTCACTCCCCTTCCCACTTTGTGTAGAACCA7950.2780332730636469No Hit
CACTAGTACCTTGCTCTTTTTCATGTGGAACCAGCGGTAGAAATTGTACT7730.2703392705386152No Hit
GGCACTCAGGAAAGTATCTCGTTGTGGGCATTAGTAGTGTAGAACCATGT7070.2472572629635199No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG7060.2469075355760185No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT7050.24655780818851705No Hit
GGAGAGCTGTAAATTCTGGCTTTTTCAGAATGCATGTAGAACCATGTCGT7000.24480917125100984No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT6930.24236107953849975No Hit
CCCTGTCCCTCTCTCTTCCTCCTCCTCCTCCTCCTCCTGTGTGTTTAGGA6900.24131189737599543No Hit
AGGAAATAATGAGCGAACCCCACTGTAGAACCATGTCGTCATTCTGGCTA6440.22522443755092902No Hit
TGCAAATGTAAGTGGTGCTCCGTTTTAGTAGCAGTTAACTGTAGAACCAT5880.20563970385084826No Hit
GGTGTGGTAGACTGGACTTCATTAGCTGGTCCTGTTGTTTGTTCTTCTTT5870.2052899764633468No Hit
TGCAAATGTAAGTGGTGCTTCGTTTTAGTAGCAGTTAACTGTAGAACCAT5750.20109324781332952No Hit
GCAAAGAAAACCAATTTTTGATGCCACCCTTACTTGTAGAACCATGTCGT5670.19829542871331796No Hit
GGCACTCAGGAAAGTATCTCTTTGTGGGCATTAGTAGTGTAGAACCATGT5600.19584733700080786No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC5500.19235006312579345No Hit
CACTCAAGAGCACAGGTACAGTTTTGTAAATTCATCTGGATAATTGTAGA5380.18815333447577612No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT5380.18815333447577612No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA5200.18185824150075017No Hit
TGTGAGTGATAAAGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAACCAT5150.18010960456324296No Hit
ATGATCCATGGACATTTGACTGGTAGCAGATACATTGGACCACTGCTTTT5100.17836096762573575No Hit
CCATGGGACTGACTTTCTGCTACCGTAGACACCTACCTGATACCCCAGAT5090.17801124023823428No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA4970.17381451158821698No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC4910.17171614726320833No Hit
CCTCCTGGCCCCTGTCCTGCCTCCCGTGGGGGTTGTCCACGCTGTAGAAC4890.17101669248820545No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT4890.17101669248820545No Hit
AGCGGGACAAGCTCCTCTACCGTAGTCTGGCTTTTTCAGAATGCATGTAG4370.15283086833813045No Hit
ACACTTCTGGGTAACTTTTCAGACTGGAATTAAGTGGAAGCCTGTAGAAC4350.15213141356312754No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT4290.1500330492381189No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG4220.14758495752560877No Hit
TCACTGGAAAATTCTAACGTGAGGTGTGGCTGTAGAACCATGTCGTCAGT4160.14548659320060012No Hit
TGTGCAGTAAAGAATGGCCAGTGTAGAACCATGTCGTCAGTGTAGATCTC4120.14408768365059435No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG3820.13359586202555107No Hit
CTGGGTATGGTGGCACATCTGTAGAACCATGTCGTCAGTGTAGATCTCGG3790.13254667986304675No Hit
TGCACGTACCACATTTAATTACCTTGCAGAGACTTGGTCTTCTCTTGGTA3720.13009858815053665No Hit
ACTAGTATTCTGAGCTGTGTGCTCAGCCAGAGAAGTGAAAGCTGTAGAAC3640.12730076905052512No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT3610.1262515868880208No Hit
GAACGAAGCTCTATCTGCCCAATTAGCATGTAGAACCATGTCGTCAGTGT3540.12380349517551069No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC3490.12205485823800347No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC3460.12100567607549916No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG3410.11925703913799193No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT3400.1189073117504905No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTAGAACACAGAGGAGAATTTATTA3380.11820785697548761No Hit
GCTGCCCCCACCATGAGTACGGTAGCAGATGTTTCTGAGAGGGCTGTAGA3380.11820785697548761No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT3370.11785812958798615No Hit
GAGTGTTCTGAAAGGAAATCTGGATAATTTCTTTTTTGAATGTGCACTGT3360.11750840220048472No Hit
TGTGCAGTAAAGAATGGCCAGTGTAGAACCATGTCGTCAGTGTACCCTAA3350.11715867481298328No Hit
TGTGCAGTAAAGAATGGCCAGTATGATTATCTAGCAAGTTCTTCATTTGT3260.11401112832547029No Hit
CTGAAAAACAAAATAAAATCTCTACCTGGCCGTCAGTGGTATTGTAGAAC3250.11366140093796887No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGACTTGGTCTGTCTAAGAACACA3080.10771603535044433No Hit
ACATGTCTTACCGAAAGGGTACACCACACTCTGCACAATTCTGTAGAACC2950.10316957931292556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)