FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354271
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA106002.9920597508686853No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC100912.8483844288694247No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG93022.62567356628118No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC92222.603091983255756No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG91722.5889784938648663No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA90802.5630096733856287No Hit
TACTCTTCTTGGCTCCAGTTGCAGGTTCTTTACCTTCCATGAGTTGTAGG86352.437399617806707No Hit
AGAAGACCAGGAAAGAACGGGAGCTGAAGTCTGGGCACAAAGACCGGAGT82412.326185321406494No Hit
GCCACAAAGTAAAAATGTTGTGTGTTTACTTTTTTGCATCTTGGCAGGCT82222.3208221954379558No Hit
ACTCTACCCTGCGATTTTCTCTCATACAAAGATCTGAATCTCTAACTTTC81842.310095943500879No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT72152.0365765191054304No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT71862.0283906952587145No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT71322.0131481267165534No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT70731.996494209235303No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC69081.9499196942453656No Hit
GTGCTCAAAAGAAGTGCTGAAGGAAGCAACCCTCCTAAACCACTGAAAAA65961.861851520446212No Hit
AATCTGTGATTCTTAGCCAACTTGAAATGAAGACTTTTCCTTTAAATATA64731.8271323365446228No Hit
GTATGAGTTTTAGAGGCTGTTAATTTGCTATAATATTAGCTACATCTGGA64161.811042958639008No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG60411.7051917882073326No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC57721.6292612152843444No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC51071.441551806385507No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG42051.186944457773851No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA40451.141781291723003No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA39721.1211755972123034No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC39051.102263521428511No Hit
AATAATGAGTTTGGTCATCTGAACTTTTAATTAATTAAGTTCATGTGTTT35591.004598174843552No Hit
AGATGATACTGCTGCCCTCGGACTGCCGAGGACACAAAGCCAGGCCTAAA32880.9281030623449281No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC32200.9089087167733175No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA31600.8919725295042495No Hit
CCAAGGAACATCTTCAGTATCTCTAGGATTCTCTGAGCATGGCAGTTTCT29760.8400348885457742No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG28800.8129369889152656No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT28650.8087029420979984No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGTAATGG24540.6926900593048824No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC24460.6904319010023401No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGTA23520.6638985409474668No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG23440.6616403826449244No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC21730.6133722489280804No Hit
GGGAAAAGAACAGGCTTCACCTATCATCGTTTTCCAAGTTATTTTTGAGA21680.6119608999889915No Hit
GAAAGTGGTGCATTGATGGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC21450.6054686948691821No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACAAGGTAATTAAATGTGGTAC21230.5992587595371904No Hit
CCCGTTTTCCCAGATATTTTAACACTGAAGAAAAGTTACTTTTGACAAGT20770.5862743492975716No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC20670.5834516514193936No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC20580.5809112233290334No Hit
TACCCTCCATGAGGCACACCTTGCTCCAGAGCGAGGTAATCATCTGCATT20250.571596320331046No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC19970.5636927662721476No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG19680.5555069424254314No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC19660.5549424028497958No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC19340.5459097696396261No Hit
GAAAGTGGTGCATTGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTCT19240.5430870717614482No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGTAA17650.4982061754984179No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGT17640.4979239057106No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA17600.49679482655932883No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGTAATG17510.4942543984689686No Hit
TGTAACCATGAGAAAGTGGGACTTCAAATACGAACCAATGTTAAGGATCT17270.4874799235613414No Hit
GTGGACAAGTTTGACATTATTGGGATTATTGGAGAAGGAACCTATGGCCA16610.46885011756536665No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG16490.465462880111553No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG15720.4437281064495824No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA15590.440058599207951No Hit
GACAAAAATCATCTCTCCGAAAAACAAGCACACAGCTCAGAATACTAGTA15280.4313082357855992No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT14870.41973517448506936No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC14600.4121138902139887No Hit
AGGTTCAACTACGCGTGTAAATTCTACTGCAAATGCAGAGACACAAGCAA13770.3886854978251113No Hit
GACAGCAGAACACACATACCAAAGTCAGTACTGAGCACAACAAGGAATGT13760.3884032280372935No Hit
AGTGGAACTGTTATTATCCCATCAAATGGCACAGCTGAGACCAAGTCTCT13590.3836046416443909No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC11800.3330783496250046No Hit
CGAACCCTCTCTGGGTGATAATTACACAAATCTTCATAGATGATATACTG11110.31360173426557636No Hit
GTAACTTGATTTGCTGTTGTATTTGCTACCGTAACCCTCAATTTGGAAGC11060.31219038532648735No Hit
GGTGCCAGTTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACCG10940.3088031478726737No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG10930.30852087808485595No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT10740.30315775211631774No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA10240.2890442627254277No Hit
GAAGTTCATCCTGTTTTAAGTCACACTGACGACATGGTTCTACAGGGAAG10010.2825520576056183No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT9810.27690666184926227No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCAAA9580.2704144567294529No Hit
ATCTGACATTTTCTATTTGTTTTTACAGATTTATAATCAGAGTCAGTCCA8730.24642152476493984No Hit
TTTTAAGATGGTGTTTAAAAGAAGTAACCCTCAATTTGGAAGCCTCTTAG8690.2452924456136686No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC8690.2452924456136686No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGTA8660.24444563625021523No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG8100.22863852813241842No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT8040.2269449094055116No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG7960.2246867511029692No Hit
TTCTGATGAGCCAAGTCTCTGTGAAACAGTAGGTATGTAAAATTTAAGTC7660.21621865746843516No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG7220.20379878680445196No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT6650.18770940889883733No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT6590.1860157901719305No Hit
GGCATCTGTATATTTATTTTAAACACTGCTACCGTAACTTTAAAGCCATA6420.18121720377902792No Hit
AAACTTAAGGTTTTGATGGCCTACCGTACACATAGGTTAACATTTACCAC6050.1707732216297693No Hit
ATTTACCCTACCAATTGCCCAGTACACTGACGACATGGTATTCGATGACT5940.1676682539637735No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGTAAT5820.16428101650995988No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGTAATGGAGTATC5730.1617405884195997No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT5460.15411930414851907No Hit
CGGCCCCTTCCGCTTTACGTCTGACGTCACGCCGCACGCCCCAGCGAGGG5420.15299022499724788No Hit
GCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAA5220.14734482924089184No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCAAGGTAATTAAA4760.13436041900127305No Hit
CAAAGTGGGAAGGGGAGTGAACATCGAATCATGGAATCCCAGACCAAGTC4620.13040864197182384No Hit
ATACCACTGACGGCCAGGTACAGAACAGGAGTGCACGGAAGACCAAGTCT4470.1261745951545568No Hit
GGGATAATGGGGTTTTCCTGTGTACACTGACGACATTTGCTGCCATACAG4340.12250508791292541No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTAGT4310.12165827854947202No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTAACCCTCAATTTGGAAGCCTC4240.11968239003474741No Hit
CCAGACTCTCCAGAACCAAAGGACACTGACGACAGGGTTCTATGATTTCA4180.1179887713078406No Hit
CATCTCCATGAGTAGGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC4180.1179887713078406No Hit
CATCTCCATGAGTAGGAAGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC4120.11629515258093381No Hit
GGTCCAGCAGCCCTTTCCGGTCTTTGGTCACATTCTTCCAACAAACCAGT3980.1123433755514846No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA3900.11008521724894219No Hit
TTTCTCCTCCCTGTGGTTCTACTGGGAGGAGGGCAGTACACAGACCAAGT3890.10980294746112439No Hit
AACTGATCACCTTGAATCTGCTTGGTGAAGAACATTCAGGCAAGACCAAG3880.10952067767330659No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGTAA3840.10839159852203538No Hit
CAAAGTGGGAAGGGGAGTGAATATCGAATCATGGAATCCCAGACCAAGTC3840.10839159852203538No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAGTAATG3790.1069802495829464No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA3760.10613344021949299No Hit
ACTCTACCCTGCGATTTTCTCTCCACTGACTATTTTATTAAACTCTCACC3740.10556890064385739No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAGT3680.10387528191695058No Hit
ACTCTACCCTGCGATTTTCTCTCATGCAAAGATCTGAATCTCTAACTTTC3640.10274620276567938No Hit
TTATGGAACAGACTTTATAGATTAAGGGACATGCTAAAAGTTTCTTGGAT3640.10274620276567938No Hit
TTCAACGTTGCCTCCTTTGCCCCCAAAAAATAAATCAATAAAAGTTTTCA3570.10077031425095477No Hit
GCCGGAACAGCCCCAGAGTGTGATGTTCCCCTTCCCTGTCCTTGCCAGAC3560.10048804446313699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)