FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences354271
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT104422.947461124393473No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG100962.849795777808514No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC95012.6818452540569226No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA91962.5957529687724934No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC88082.4862322910991868No Hit
GGAGGAAGTCTTCTACCAGGCATATTCATGCGCTTGAACTAGTAGTCAGT84042.372195296820795No Hit
TGGGAGGTGAGAGTTTAATAAAATAGTCAAGGACACATAGCATTAACTGA78982.2293667841849887No Hit
CTTGGCCATAAGAAGCTCCCGAGGGGCTATCATCTTGTTTGGAGCTGTCA77752.194647600283399No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG77422.1853326972854115No Hit
TCAAAAGGAGATACTTACACAACAGGAAAGACTTTGGGAAACACAACACT75942.143556768688377No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC71652.0224630297145407No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC71092.0066559215967437No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT70932.0021396049916587No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT69861.9719367376951544No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG65261.842092635298966No Hit
CAGCTGCCTACTTCCTCCATGGCCAGCAAGAGCTTTTCGTAGACTGTCCA62851.774065616434876No Hit
AAACTTGGCATGAAAGAAATTGGTATAAGCCAGGAGCAGTGCTGAGACTC61441.734265576352566No Hit
AGGTTGCTTGTGTCTCTGCATTTGCAGTAGAATTTACACGCGTAGTTGAA61041.7229747848398542No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT59861.689666949877354No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA57761.6303902944356157No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC47881.3515077440716288No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG41121.1606933675067956No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT40001.129079151271202No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT39471.1141188525168586No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG38941.099158553762515No Hit
CCGCCCATCAATATCAAAATCCCACTCTAATCAAATAATACTGACTTTGA34690.9791938939399499No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG32300.9117314146514957No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT31780.89705338568497No Hit
GACGGCTGCTCTTTTCGTTCTGTCGCCTGCCCGATGGACGAGCCTCCCGG31230.881528547354991No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCCTAATCCAT30240.8535838383610286No Hit
CAGATGGGCTGGAAGTAAGGAAACATGTAATGATAGGCGGACTCCCAGCA29830.8420107770604989No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT28770.812090179551812No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT24610.694665947819607No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC24400.6887382822754332No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA23530.6641808107352846No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC23260.656559526464204No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG21810.6156304072306229No Hit
CAAAAATAACTTGGAAAACGATGATAGGTGAAGCCTGTTCTTTTCCCTGT21520.6074445833839067No Hit
GAGACCTACATCAGGCCTTCCATCAATGCACCACTTTCTGTAGAACCATG21430.6049041552935465No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG20550.5800644139655801No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA20480.5780885254508554No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG20310.5732899390579529No Hit
AATGCAGATGATTACCTCGCTCTGGAGCAAGGTGTGCCTCATGGAGGGTA19980.5639750360599655No Hit
TGCACGTACCACATTTAATTACCTTGTCTTCTCTTGGTATTAGTGCTTGA19950.563128226696512No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC19890.5614346079696052No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG19870.5608700683939696No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG19310.5450629602761727No Hit
GAGACCTACATCAGGCCTTCATCAATGCACCACTTTCTGTAGAACCATGT19180.5413934530345413No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT17880.5046983806182272No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT17560.4956657474080577No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT17470.49312531931769743No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG17410.49143170059079067No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA16710.4716728154435446No Hit
CAAAACACCTCAGAATTTACTTTTGCTAAATCCACTTAAGATATTAGTAT16690.47110827586790904No Hit
AGAAAGGTGAAAAATAAGAGAACACTTTACCTGTCCTTGGGAGTCAAAAA15730.44401037623740014No Hit
ACTAGTATTCTGAGCTGTGTGCTTGTTTTTCGGAGAGATGATTTTTGTCT15180.42848553790742117No Hit
ACATAATTCTAACCTATAAAGACTTTTTTCACTTAGAAGCTGAAACATAA14940.421711062999794No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT14940.421711062999794No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG14880.4200174442728871No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA14650.4135252391530777No Hit
CCCCAAAACACAGTAACCCAAATACTCACCATATTGTTAACATTCTTTAA14320.4042103361550903No Hit
CAGCTGTGCCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGT13530.3819110229174841No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT13450.37965286461494163No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG13260.37428973864640347No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA11800.3330783496250046No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTAGAACCATGTCGTCAGT11110.31360173426557636No Hit
CACCATCCAAAGGACAACCTTATTAAGCTCGTCACTGCAGGTGGGGGCCA11080.312754924902123No Hit
AAGAGGCTTCCAAATTGAGGGTTACGGTAGCAAATACAACAGCAAATCAA10770.3040045614797711No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT10690.30174640317722873No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT10640.3003350542381397No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG10090.2848102159081607No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC9750.2752130431223555No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTGACTTAA9520.2687208380025461No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGAGACTTGGTCTGTCTAAGAACA9510.2684385682147283No Hit
CAGTCAAGAGTCATCGAATACCATGTCGTCAGTGTACTGGGCAATTGGTA9260.26138182351928324No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA8630.24359882688676182No Hit
AAGAGGCTTCCAAATTGAGGGTTACTTCTTTTAAACACCATCTTAAAATG8600.24275201752330844No Hit
CTGAGTAAGTGGCAAGAAAATTACCTGCGTGCTGCATCAAAGTTGCTTTT8400.2371066217669524No Hit
AGACACCATGTGTGATGAACATTTTGTGTAATTTTGGGTTGATTCCATCA8170.230614416647143No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC7970.22496902089078702No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT7950.22440448131515142No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG7890.2227108625882446No Hit
AAGACTTAAATTTTACATACCTACTGTTTCACAGAGACTTGGCTCATCAG7360.20775056383390117No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT7150.20182289828972735No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT6580.18573352038411273No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG6440.18178174335466352No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT6080.1716200309932227No Hit
TGGCTTTGCATAATATGGCTTTAAAGTTACGGTAGCAGTGTTTAAAATAA6060.1710554914175871No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC5930.1673859841759557No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGGCCATCAAAACCTTAA5650.15948243011705726No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT5430.1532724947850657No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT5230.14762709902870966No Hit
TCCTCAGCAGTCTGGAACCCCGCAGACACCAGCTTCACCCGCACCGCTGG4900.13831219603072223No Hit
GGGATTCCATGATTCGATGTTCACTCCCCTTCCCACTTTGTGTAGAACCA4700.13266680027436623No Hit
TCCGTGCACTCCTGTTCTGTACCTGGCCGTCAGTGGTATTGTAGAACCAT4500.12702140451801022No Hit
GTCTCTGTATGGCAGCAAATGTCGTCAGTGTACACAGGAAAACCCCATTA4360.12306962748856103No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG4300.12137600876165422No Hit
TGCACGTACCACATTTAATTACCTTGAGACTTGGTCTTCTCTTGGTATTA4290.12109373897383642No Hit
CGGGAGGTGAGAGTTTAATAAAATAGTCAAGGACACATAGCATTAACTGA4220.1191178504591118No Hit
GAGACCTACATCAGGCCTTCTTCCTACTCATGGAGATGTGTAGAACCATG4150.1171419619443872No Hit
GAGACCTACATCAGGCCTTCATCCTACTCATGGAGATGTGTAGAACCATG4110.11601288279311602No Hit
AAGAGGCTTCCAAATTGAGGGTTACGGTAGCAGAGACCTGTGGGAAGCGA4090.11544834321748039No Hit
AAGACATCACTGAAATCATAGAACCCTGTCGTCAGTGTCCTTTGGTTCTG4000.1129079151271202No Hit
ACTGGTTTGTTGGAAGAATGTGACCAAAGACCGGAAAGGGCTGCTGGACC3920.1106497568245778No Hit
TGCCTGAATGTTCTTCACCAAGCAGATTCAAGGTGATCAGTTTGTAGAAC3910.11036748703675998No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT3910.11036748703675998No Hit
GTGTACTGCCCTCCTCCCAGTAGAACCACAGGGAGGAGAAATGTAGAACC3910.11036748703675998No Hit
GGGATTCCATGATTCGATATTCACTCCCCTTCCCACTTTGTGTAGAACCA3900.11008521724894219No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT3860.108956138097671No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT3810.107544789158582No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT3790.1069802495829464No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG3720.10500436106822178No Hit
GCAGATGTCTTTGAGTGAGTAGAGGGGGTGGGGGAGATGTAGAACCATGT3710.10472209128040398No Hit
GGCAAGGACAGGGAAGGGGAACATCACACTCTGGGGCTGTTCCGGCTGTA3560.10048804446313699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)