FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333746
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC82232.4638497540045425No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA80172.4021261678042585No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA76412.2894656415357786No Hit
CTAAATACGTTTCACAGGTAGAAAAATAAAACAATTGTGTCAACCAGAGA71012.1276659495544514No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC68092.040174264260845No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC67482.0218968916481392No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT60821.8223439382045028No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT58081.7402455759769404No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA58061.739646317858491No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC57941.736050769147795No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC57391.7195711708904375No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC55851.6734282957698368No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG49471.4822649559844912No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAAAATGAAA48311.4475079851144284No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA48041.439418000515362No Hit
GTGCAGGCTGATTTTCTTTTTCCTGTGTATCTTCTACCAGGTGCTTGGGC46981.4076573202375458No Hit
GATTCCCGCGCCCAGAGCATGGTTCTACAGAGTCCCACGACCCCCTCAGA43841.3135737956409965No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC43561.3051841819827052No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC43091.2911016161991453No Hit
TCTCCCCCACCCCCTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT42671.2785171957117087No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT40891.225183223169716No Hit
AGATGAGTCATATTTGTGGGTTTTCACCACTGACTCTGATCCAGAAGACC39471.1826358967598114No Hit
AGTGGAACTGTTATTATCCCATCAAATGGCACAGCTGAGACCAAGTCTCT38941.1667555566209031No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT37601.1266052626847962No Hit
GAAAGTGGTGCATTGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTCT37051.1101256644274389No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC35831.073570919202028No Hit
GAAAGTGGTGCATTGATGGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC33240.9959669928628357No Hit
TCAAAGGGCTCCACTGGTTTTTCTTATCTCTTTCATTTGATTAGACCAAG32640.977989249309355No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGAATGGA32280.9672026031772665No Hit
TACCCTCCATGAGGCACACCCTAAGCCAGAATTTACAGCTCTCCAGACCA31860.9546181826898301No Hit
GGGGTCAGAGGCAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGT29200.874916852936065No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA29140.8731190785807171No Hit
GAAAAACAATTTATATCTGTCAGTGAATCCACTAGGACTGCTCCCACCAG28430.8518454153757647No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC27900.8359650752368568No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGAA26610.797312926596873No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT26310.7883240548201326No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG26150.7835299898725377No Hit
GGTGCCAGTTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACCG24900.7460763574694529No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC23940.7173119677838836No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGA23330.699034595171178No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA22260.6669742858341373No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA22220.6657757695972386No Hit
GGCTCCATAGGAAAGTAACAGCAACAGAGGAGGGGGAGACCAAGTCTCTG21930.6570865268797229No Hit
GTTGTTTCAGAACACCGAAAGCTCAAGAAGCATGTCATAGACCAAGTCTC21560.6460002516884098No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGAAT21060.6310187987271757No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAAAGA21020.629820282490277No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC20590.6169362329436159No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA20210.605550328693078No Hit
GACTACAGATGTGCCACCATACCAACTGCTCTGGAACATGTTAGACCAAG20200.6052506996338533No Hit
TCAAAGGGCTCCACAGTTTTTCTTATCTCTTTCATTTGATTAGACCAAGT19740.5914677629095181No Hit
GTTGTTTCAGAACACCGAAAAGCTCAAGAAGCATGTCATAGACCAAGTCT19520.584875923606575No Hit
AGTTCCATTTAGGTTGCTGGTGAAGAACATTCAGGCAAGACCAAGTCTCT19220.5758870518298346No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC18970.5683963253492177No Hit
GGGGTCAGAGGAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGTA18640.5585085663948032No Hit
TGTGCCTCCAAACTTACAGGTTACGGTAGCAGAAAGTCAGTCCCATGGAG18000.5393323066044237No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG16390.49109202806925023No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC16380.4907923990100256No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA16060.4812042691148358No Hit
TCTCCCCCACCCCTTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT15230.4563350571991874No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC14540.43566065211268445No Hit
CTCCTCCACAGCTCGCTACCGTAACATCAGATCCTTACCTGTCAGGAGAC14360.4302673290466403No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT14240.4266717803359441No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG14090.42217734444757393No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC14050.42097882821067517No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGAATGG13870.415585505144631No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT13670.40959292396013736No Hit
AGTTCCATTTAGGTTGCTGGTGGAGAACATTCAGGCAAGACCAAGTCTCT12370.370641146260929No Hit
TGGGGAAAAATATGCTTTGATCTACAGAAAAACATAGAAATCAGAGACCA12120.363150419780312No Hit
TGGGGAAAAATATGCTTTGAGCTACAGAAAAACATAGAAATCAGAGACCA11930.35745746765504305No Hit
ATTACAACCCATCTGAGTGGAGCCCTGGCTTCTCAGGCAGACCTGGTGTC11820.3541615480035716No Hit
CCAAATCTGCTTTCTTGATAAAATCCTCTGAATGGCATGCAGACCAAGTC11760.3523637736482235No Hit
CTGATGTTGAATTAACCAAAAATATTCCCTCTGCGTGTTCTCATAAGACC11630.34846859587830264No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC11110.3328878847986193No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTACCTTCAGAGCTAGGGAAAAA10840.32479790019955296No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT10430.3125131087713411No Hit
GAGATTGATGACCAAAAGAACTGCACACTGACGACATGGTTCTCCTCAGA10250.30711978570529685No Hit
GGAAAACCAGCGGAACCTCCACACTGACGACAAGGATCTAGCTGCTGATG10010.2999286882839045No Hit
TCTACCCACTCTCTTTTCAGTGCTTACGGTAGCAGAACTGTCAAATGACC9970.2987301720470058No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG9760.2924379618032875No Hit
AAAGCAGAAGCCTTAGAAACTCTGCTACCGTAACAGACTTACAGATCCAT9430.2825502028488731No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACAAAGGG8720.26127653964392084No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTAAGGTAATTAAAT8700.2606772815254715No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC8690.2603776524662468No Hit
TCTTAAATGGCTACGACCCAGACCAAGTCTCTGCTACCGTAGAATGGAAG8650.2591791362293481No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGAA8240.2468943448011362No Hit
CATCTCCATGAGTAGGAAGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC8090.24239990891276603No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG8040.2409017636166426No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTATCTCAGT7930.23760584396517112No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGAATGGAAGAATC7340.21992772947091502No Hit
TGCATTCTGAAAAAGCCAGAATTTACAGCTCTCCAGACCAAGTCTCTGCT7290.21842958417479158No Hit
AGGCTATTTTCCTTTTATACCTTGGGCGGAGCAGAGATTGGCCACTACAA7140.2139351482864214No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT7110.21303626110874738No Hit
GCTTTCACTTCTCTGGCTGAACACACAGCTCAGAATACTAGTAGACCAAG7050.21123848675339932No Hit
GGCTTCCACTTAATTCCAGTCTAAAAAGTTACCCAGAAGTGTAGACCAAG6700.20075146968053548No Hit
AGATGAGTCATATTTGTGGGTTTTCATACAGCTTCCCTCTTTAAGATGTC6690.2004518406213108No Hit
GCAGGAAGGAAAAGGAACCTGTACACTGACGACATGGTTCAGCTTTCGTT6550.19625703379216533No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA6430.19266148508146913No Hit
AATCTGTGATTCTTAGCCAACTTGAAACGGACTTGCTATTTACTGAGACC6400.1917625979037951No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT6350.19026445260767172No Hit
AGTAAGGGTGGCATCAAAAATTGGTTTTCTTTGCAGACCAAGTCTCTGCT6130.18367261330472875No Hit
GTCCTCCAGAGCACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAG5910.1770807740017858No Hit
CCATCATGTGAGTCATCAGAACCTACACTGACGACATGGAGGAAGTAGGC5890.17648151588333644No Hit
CACTTAATGAGACCAACAGTAACACCTTTATCACTCACAAGACCAAGTCT5830.17468374152798835No Hit
TCCACAAACAGAACAAGATGCTTACGGTAGCAGAAGCCTTTTTAGCCCTA5680.17018930563961815No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGAATG5460.1635974663366752No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA5340.16000191762597904No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA5330.15970228856675436No Hit
TTTTAAGATGGTGTTTAAAAGAAGTAACCCAGAAGTGGGGTTTAGGGGCT5320.1594026595075297No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTAGA5290.15850377232985563No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGAAT4980.14921527149389058No Hit
TGCTGGAAATGTTTTAGATTCTGGTTACGGTAGCAGAAGCCTTTTTAGCC4950.14831638431621652No Hit
AGGCTGGGTTTGCACTGACGACTTGGTATTGCTCCCACATGGAGCTAGAC4910.1471178680793178No Hit
CATCTCCATGAGTAGGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC4690.14052602877637485No Hit
GCCACACCTCACGTTAGAATTTTCCAGTGAAGACCAAGTCTCTGCTACCG4630.13872825442102676No Hit
CAGCAATGTGGCTTTAAGTAGAACATATCATCTGGACCCTTTTCAGACCA4600.13782936724335274No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAGA4560.13663085100645403No Hit
TCCTCCCCTCTTAGCTGTCTTAGTGAAAGGTATGATGAAGCTAGACCAAG4530.13573196382877997No Hit
CAGCAATGTGGCTTTAATTAGAACATATCATCTGGACCCTTTTCAGACCA4290.12854086640738766No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC4270.12794160828893827No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGAATG4140.12404643051901745No Hit
AGCAATCTGCTTGTGTGTGCGTGAGACCAAGTCTCTGCTACCGTAGAATG4020.1204508818083213No Hit
GATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTAGAATGGAAG3920.1174545912160745No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA3850.11535718780150174No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG3840.11505755874227706No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTAGAATGG3810.11415867156460302No Hit
TTTCTTACCCTGTGCAGAGCATTACACTGACGACATGGTTCTACAGGGAA3590.10756683226166007No Hit
GAGATTGATGACCAAAAGAACTGCACACTGACGACATGGTTCTACAGAGA3550.10636831602476135No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA3360.10067536389949243No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)