FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences333746
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG82902.483924900972596No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG80942.4251976053645588No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT79452.3805528755400815No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT75532.263098284324007No Hit
CTGGTTGACACAATTGTTTTATTTTTCTACCTGTGAAACGTATTTAGTGT70752.11987559401461No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA67882.033882054017127No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA67672.0275898437734083No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT60421.8103587758355157No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG58191.743541495628412No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC57551.7243652358380324No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA56801.7018930563961816No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA55961.6767242154213085No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT54501.6329783727745053No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC49661.48795790810976No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTTGGTCTGTCTAAGAACACAGAGG48511.453500566298922No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA48081.4406165167522607No Hit
ATTAGGATGTCTGGCACATGCACAGGACAACCAAGTTCAAGAACCTCTCA47221.4148484176589382No Hit
GCAGATGTCTTTGAGTGAGTAGAGGGGGTGGGGGAGATGTAGAACCATGT44531.3342482007274994No Hit
GAGGGGGTCGTGGGACTCTGTAGAACCATGCTCTGGGCGCGGGAATCTGT43591.3060830691603793No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT40681.2188910129259978No Hit
TCTGGATCAGAGTCAGTGGTGAAAACCCACAAATATGACTCATCTTGTAG39501.1835347839374855No Hit
CAGCTGTGCCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGT39111.171849250627723No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT37541.124807488329448No Hit
GAGACCTACATCAGGCCTTCATCAATGCACCACTTTCTGTAGAACCATGT37381.1200134233818533No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG36031.0795635003865216No Hit
GAGACCTACATCAGGCCTTCCATCAATGCACCACTTTCTGTAGAACCATG33381.0001617996919814No Hit
AATCAAATGAAAGAGATAAGAAAAACCAGTGGAGCCCTTTGATGTAGAAC32430.9716970390656366No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC32200.9648055707034691No Hit
GGAGAGCTGTAAATTCTGGCTTAGGGTGTGCCTCATGGAGGGTATGTAGA31960.9576144732820767No Hit
ACACCGGATATCTGCTTGCCTCTGACCCCTGTAGAACCATGTCGTCAGTG29130.8728194495214925No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT29010.8692239008107963No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA27840.8341673008815087No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA26760.8018073624852432No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC26430.7919196035308289No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC26070.7811329573987403No Hit
GTGTCCTGCTTATTTTTCTCACATTCTTCCGTACTGGCCTGGGAACTCTC25400.7610578104306869No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTAGAACCATGTCGTCAGT25310.7583611488976647No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG23720.7107201284809406No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG23270.69723682081583No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCCTAATCCAT23160.6939409011643585No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT22400.6711690926632828No Hit
CCCCCTCCTCTGTTGCTGTTACTTTCCTATGGAGCCTGTAGAACCATGTC21730.6510939456952293No Hit
ATGACATGCTTCTTGAGCTTTCGGTGTTCTGAAACAACTGTAGAACCATG21380.6406069286223656No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT21200.6352136055563212No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAAAATCAAACAGAGT21110.6325169440232992No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT20770.62232955600966No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG20320.6088462483445495No Hit
AACATGTTCCAGAGCAGTTGGTATGGTGGCACATCTGTAGTCTGTAGAAC20080.6016551509231571No Hit
TATGGTCCTCAGAAAAGTGCACCAGATCATCTTCCATTTGTAATACTTCT19930.5971607150347868No Hit
AATCAAATGAAAGAGATAAGAAAAACTGTGGAGCCCTTTGATGTAGAACC19650.5887711013764959No Hit
ATGACATGCTTCTTGAGCTTTTCGGTGTTCTGAAACAACTGTAGAACCAT19440.5824788911327776No Hit
TGCCTGAATGTTCTTCACCAGCAACCTAAATGGAACTTGTAGAACCATGT19180.5746885355929359No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG18870.5654000347569709No Hit
ACACCGGATATCTGCTTCCTCTGACCCCTGTAGAACCATGTCGTCAGTGT18690.5600067116909266No Hit
CCATGGGACTGACTTTCTGCTACCGTAACCTGTAAGTTTGGAGGCACATG17830.5342386125976042No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG16470.49348906054304775No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT16260.48719685029932946No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG16220.48599833406243076No Hit
GCAGATGTCTTTGAGTGAGTAGAAGGGGTGGGGGAGATGTAGAACCATGT15320.45903171873220955No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG14470.43356324869811175No Hit
CCTGACAGGTAAGGATCTGATGTTACGGTAGCGAGCTGTGGAGGAGTGTA14350.4299676999874155No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT14260.42727103845439346No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA14130.42337586068447264No Hit
TTTGAAACAGAAGCACATTAATATTTTATAAGCCATGTAGAACCATGTCG13910.4167840213815297No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT13780.4128888436116088No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT13660.4092932949009127No Hit
TGCCTGAATGTTCTCCACCAGCAACCTAAATGGAACTTGTAGAACCATGT12450.37303817873472644No Hit
CTGATTTCTATGTTTTTCTGTAGATCAAAGCATATTTTTCCCCATGTAGA12060.36135264542496387No Hit
GCATGCCATTCAGAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA11840.35476080612202093No Hit
CTGATTTCTATGTTTTTCTGTAGCTCAAAGCATATTTTTCCCCATGTAGA11770.35266340270744817No Hit
TATGAGAACACGCAGAGGGAATATTTTTGGTTAATTCAACATCAGTGTAG11600.3475697087006286No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA11040.33079048138404654No Hit
AGGGCCTATTAGCCATATCTCCCAGCTCTCAACAGGAGGTGGAGAGGCAG10890.3262960454956763No Hit
CACCATCAGCAGCTAGATCCTTGTCGTCAGTGTGGAGGTTCCGCTGGTTT10070.30172646263925257No Hit
TTAAATGGAGTCATCTGAGGAGAACCATGTCGTCAGTGTGCAGTTCTTTT9840.2948349942770849No Hit
ACTTTTTCCCTAGCTCTGAAGGTACGGTAGCAGACCTTTGTGCTTTTATC9690.29034055838871475No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT9530.2855464934411199No Hit
TGGTCATTTGACAGTTCTGCTACCGTAAGCACTGAAAAGAGAGTGGGTAG9370.28075242849352505No Hit
AGCATTATGGATCTGTAAGTCTGTTACGGTAGCAGAGTTTCTAAGGCTTC8970.26876726612453783No Hit
GGGTCGTAGCCATTTAAGATGTAGAACCATGTCGTCAGTGTAGATCTCGG8780.2630743139992689No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA8610.25798061999244937No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA8320.2492913772749336No Hit
GCAAAGAAAACCAATTTTTGATGCCACCCTTACTTGTAGAACCATGTCGT8160.24449731232733876No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC7990.23940361832051918No Hit
TGCACGTACCACATTTAATTACCTTAGACTTGGTCTTCTCTTGGTATTAG7990.23940361832051918No Hit
GAGACCTACATCAGGCCTTCTTCCTACTCATGGAGATGTGTAGAACCATG7970.23880436020206983No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT7610.22801771406998136No Hit
TGCACTGGTAAAGAAAACTGAGATAGCAGAGACTTGGTCTTCTCTTGGTA7590.227418455951532No Hit
GGAGAGCTGTAAATTCTGGCTTTTTCAGAATGCATGTAGAACCATGTCGT7480.22412253630006052No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT7050.21123848675339932No Hit
ACTAGTATTCTGAGCTGTGTGTTCAGCCAGAGAAGTGAAAGCTGTAGAAC7020.21033959957572523No Hit
TAAGGAGAATGATTTGTAGTGGCCAATCTCTGCTCCGCCCAAGGTATAAA6810.204047389332007No Hit
CAGTAAATAGCAAGTCCGTTTCAAGTTGGCTAAGAATCACAGATTTGTAG6560.19655666285139No Hit
ACACTTCTGGGTAACTTTTTAGACTGGAATTAAGTGGAAGCCTGTAGAAC6520.19535814661449125No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA6480.19415963037759254No Hit
GCTTTCAAAACGAAAGCTGAACCATGTCGTCAGTGTACAGGTTCCTTTTC6420.19236185602224445No Hit
AGAGACATCTTAAAGAGGGAAGCTGTATGAAAACCCACAAATATGACTCA6380.19116333978534575No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT6260.18756779107464958No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTGCTCTGGAGGACTG5940.17797966117945982No Hit
CAGCTGCCTACTTCCTCCATGTCGTCAGTGTAGGTTCTGATGACTCACAT5920.17738040306101047No Hit
TGTGAGTGATAAAGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAACCAT5790.17348522529108962No Hit
TTAAATGGAGTCATCTGAGGAGAACCATGTCGTCAGTGTACAGTTCTTTT5700.1707885637580675No Hit
AGGGCTAAAAAGGCTTCTGCTACCGTAAGCATCTTGTTCTGTTTGTGGAT5660.1695900475211688No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC5510.1650956116327986No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG5320.1594026595075297No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA5300.1588034013890803No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG5220.1564063689152829No Hit
AAAAGCCCCTAAACCCCACTTCTGGGTTACTTCTTTTAAACACCATCTTA5190.15550748173760884No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT4890.14651860996086843No Hit
AGCTCCATGTGGGAGCAATACCAAGTCGTCAGTGCAAACCCAGCCTTGTA4870.14591935184241908No Hit
AGGGCTAAAAAGGCTTCTGCTACCGTAACCAGAATCTAAAACATTTCCAG4740.14202417407249826No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAATGCTC4680.14022639971715017No Hit
GAAAAGGGTCCAGATGATATGTTCTACTTAAAGCCACATTGCTGTGTAGA4670.13992677065792547No Hit
GAGACCTACATCAGGCCTTCATCCTACTCATGGAGATGTGTAGAACCATG4670.13992677065792547No Hit
AGCTTCATCATACCTTTCACTAAGACAGCTAAGAGGGGAGGATGTAGAAC4550.13633122194722935No Hit
TCACTGGAAAATTCTAACGTGAGGTGTGGCTGTAGAACCATGTCGTCAGT4460.1336345604142072No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG4410.1321364151180838No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA4310.129140124525837No Hit
GAAAAGGGTCCAGATGATATGTTCTAATTAAAGCCACATTGCTGTGTAGA4250.12734235017048892No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC4150.12434605957824213No Hit
CACGCACACACAAGCAGATTGCTTGTAGAACCATGTCGTCAGTGTAGATC4020.1204508818083213No Hit
CTGGGTATGGTGGCACATCTGTAGAACCATGTCGTCAGTGTAGATCTCGG3900.11685533309762514No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT3840.11505755874227706No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT3790.11355941344615367No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT3770.11296015532770429No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT3400.10187388013639116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)