FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352873
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT84472.393779064989387No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC81402.3067789261292306No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA76542.169052321940188No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC73862.0931043179840905No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA73682.0880033326437557No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA72622.0579641967506723No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA69811.9783321478265552No Hit
ACTCTACCCTGCGATTTTCTCTCATACAAAGATCTGAATCTCTAACTTTC65251.849107185871404No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC64741.8346543940737887No Hit
ACCCGAACGTGATGAAAAGATCAAAGAACCTACTCTGTTGGGTTTTCATA62221.7632405993090998No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG61581.7451037625434647No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA61121.7320679111181643No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC60471.713647686278066No Hit
TCAGAAGCTGACATTTAAGGGAGGGAGGAGAAGCAGAGAGGGAGACCAAG59961.6991948944804505No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT57661.634015637353949No Hit
TCTTGCTTTTTTATTTCAGGATGCTTACAATTACTTCCAGGAAGACTTTG56571.6031263372374764No Hit
GCTGTTCTTTTTGGCTTCATTTGTATTACTGAGTATTTTTCTCATAGAAA54751.5515497076852012No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA52151.477868808324808No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG51401.4566147027400793No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT49531.4036211328154888No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT49441.4010706401453215No Hit
CACGTTTTTCCCATCTAGCAATTCCATGCACTTCTCATTTCTGTTGAAGT49291.3968198190283756No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG48811.3832171914541491No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA46241.3103864563171452No Hit
ACTCACTCCATTGTTCTTGCTTTTGCTTTGTTTTCAGCCGACTTTGTGGT44511.2613603194350376No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA42621.207799973361521No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG42451.202982376095649No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG40131.1372363428202215No Hit
TTGTCAGATTTAACTTTTTTGGAAGTTGCGAAAGCTCAAGAAGCATGTCA39101.1080473711505272No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC34440.9759885284507457No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC34400.9748549761528936No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT34330.9728712596316522No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT33990.9632360650999084No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAAAGA32820.9300796603877316No Hit
TCCTTGGCTCACAGTCTGGCACACAGATAACAAAAGTGCTCCCACTTCAT30870.8748189858674368No Hit
CTGGTGTTGTTGGGCAGTGTTCAGGTTGCAAAACCCCTAATCTAGACCAA30170.8549818206550233No Hit
GCAGAACATTTTGTTTCCTCACTACTGACGACATGTTTCTTCTATAACCC26890.7620305322311427No Hit
GCTTTCACTTCTCTGGCTGAATCTACTTCTTTTATAGTTCTAATTTCAAA26230.7433269193165813No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA26110.7399262624230247No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG25780.730574455965744No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTATCCCACAGACTGATATGG25620.7260402467743353No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATA25070.7104539026788674No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC24860.7045027531151434No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC24530.6951509466578627No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC23820.6750303933709861No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG23800.67446361722206No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATAT22510.6379065556163266No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATATGGTA21270.6027664343829082No Hit
GAAAGTGGTGCATTGATGGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC21240.6019162701595191No Hit
GAAAGTGGTGCATTGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTCT20580.5832126572449579No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA20410.578395059979086No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC20280.5747110150110664No Hit
TGAAGTGGACTGTTTATCTTCTTCACTTACTAGAGACAATTCAATGTCTG20250.5738608507876771No Hit
AGCAGATTTCATGTGCCTAGAAGTACACTGACGACATGGTTCTACAAAAG19600.5554406259475788No Hit
TTGGAAGAAGAAATTATCTCAAAAGGAATTAAACTTGTGATTCTTGACTC19260.5458054314158352No Hit
AGTGGAACTGTTATTATCCCATCAAATGGCACAGCTGAGACCAAGTCTCT19190.5438217148945939No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC18870.5347532965117762No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG18690.5296523111714413No Hit
CCATTTTCTTCACACCTGTCGTGAAGGAAACCAGCATGCAGCTGAACTTC18400.5214340570120127No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC17500.4959291303103383No Hit
TCTCCCCCACCCCCTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT17270.4894112045976881No Hit
GTTAGAAGGCTGGCTCCCATGCTGTTCTAACACAGCTTCTAGTTCAGCCA16670.4724079201299051No Hit
TGGTGATAGATGACTTGCTGCTTGTGGGGTTCGCTCATTATTTCCTAGAC15550.44066845579004343No Hit
GATTTTATGAGACAACAGAAGCATTATACTGCTTTTTTGATGCATAAAGC15160.42961632088598445No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATATG15090.42763260436474315No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATATGGT14320.4058117226310882No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA14210.4026944538119947No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC14120.40014396114182726No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT13960.3956097519504184No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA13940.39504297580149234No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTC12910.36585400413179814No Hit
GGTGCCAGTTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACCG12430.3522513765575717No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT11450.32447934526019273No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA11250.3188115837709317No Hit
TCAAATGTAGCAAATCAGAAGCCCTTTGAGAGTGGAAGTGACAAAATCTC11090.314277374579523No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC10210.28933922402677453No Hit
AACTGATCACCTTGAATCTGCTTGGGAGAAGGAATATTTGATGGTCAACC10080.28565517905875487No Hit
CCTCCCTGATGTACCACCAAGACCAAGTCTCTGCTACCGTATATGGTAAG9980.28282129831412434No Hit
GCTATTCCTACCATTCTGATGAGGTTACGGTAGCATACCCACAAACTGTA9940.28168774601627217No Hit
GTTAGAAGGCTGGCTCCCACACTGACGACATGGTTCTACAGGGAAGCTCT8940.2533489385699671No Hit
GAGATTGATGACCAAAAGAACTGCTACACTGACGACATGGTTCTCCTCAG8650.24513068441053862No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATAT7800.22104269808117935No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA7790.22075931000671628No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG7790.22075931000671628No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGAAGGTAATTAAATGTG7660.21707526503869662No Hit
CATCTCCATGAGTAGGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC7530.21339122007067698No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC7190.2037560255389333No Hit
CATCTCCATGAGTAGGAAGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC7180.20347263746447022No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAAAAGAATGGCAGACTGACAG6930.19638793560289394No Hit
TCTCCCCCACCCCTTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT6290.17825109883725873No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT5840.16549863548642146No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT5260.14906212716756453No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC5040.14282758952937744No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATATGG5040.14282758952937744No Hit
GCCTGCTGAAAATGCCAGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA4990.14141064915706217No Hit
AGTTCCATTTAGGTTGCTGGTGGGAAGTTAGTGCATTTCATAACAATTTT4990.14141064915706217No Hit
GGGAAAAGAACAGGCTTCACCTATCATCGTTTTCCAAGTTATTTTTGAGA4790.13574288766780115No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATATGGTAAGGATC4720.13375917114655983No Hit
TGCAGATTTCATGTGCCTAGAAGTACACTGACGACATTGATTAGACTGTT4630.13120867847639237No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATATGGT4490.12724124543390963No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT4360.12355720046588999No Hit
GTTGTTTCAGAACACCGAAAGCTCAAGAAGCATGTCATAGACCAAGTCTC4330.12270703624250084No Hit
CGCCCGGCCTTCATTTTCTTAAATAACTATTTTGTTGTTTTTACTTTTTA4260.12072331972125948No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG4210.11930637934894424No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG4160.11788943897662897No Hit
GCCTGCTGAAAATGACTGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA4130.11703927475323983No Hit
AAAGCAAAGGCCAATCAAGTGAAGAAAGACCAAGTCTCTGCTACCGTATA4040.11448878208307238No Hit
AGGACGAAAACTGGACATTCTCACACTGACCACATTTAGCTGGGACACAT4010.11363861785968322No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG3960.11222167748736799No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA3940.11165490133844187No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATAACAGGACCA3860.10938779674273748No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA3800.10768746829595917No Hit
GTTGTTTCAGAACACCGAAAAGCTCAAGAAGCATGTCATAGACCAAGTCT3790.10740408022149611No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT3720.10542036370025476No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTATA3720.10542036370025476No Hit
TCGGGGGCCTACAAGAGTCTGACAGAGTTCTCATTACCCAAGACCAAGTC3680.10428681140240256No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT3660.10372003525347646No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC3630.10286987103008732No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)