FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352873
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG83302.36062266027721No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG78992.2384824001836354No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA77012.1823715614399513No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT76262.1611174558552224No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA73522.083469123452347No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA73072.0707166601015095No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA70521.9984527011134317No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT69231.961895639507698No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT61191.7340516276394058No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT60411.7119473578312876No Hit
CCCTCTCTGCTTCTCCTCCCTCCCTTAAATGTCAGCTTCTGATGTAGAAC59491.6858756549806873No Hit
TGGGAGGTGAGAGTTTAATAAAATAGTCAAGGACACATAGCATTAACTGA59361.6821916100126673No Hit
ACTTTAGGGTCTTTGCCCATTGATGGCTAAAACTGGTGATTTCACTAGTA57501.62948142816254No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC57251.6223967263009638No Hit
CCAGTACTAATGAAGTGGGCTCCAGTATTAATGAAATAGGTTCCAGTGAT55361.5688363802274472No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA51911.471067494537695No Hit
CACGCACACACAAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACG49231.3951194905815973No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT49051.3900185052412624No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT49011.3888849529434102No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC46061.3052854709768102No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT46021.304151918678958No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT45321.2843147534665447No Hit
CGAGATGTTCATTTTCCCCAAACTCACAAAGAGAATTCTTCTCGTAGACG43701.2384058854035305No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC43141.2225361532335997No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGTAGCAGAGACTTGGTCTGTCTA41161.1664253144899157No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG39751.1264675959906256No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACATAGTGAAATGTAG39391.1162656253099557No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT39291.1134317445653252No Hit
GGCGACACTAATATTTTTCCCACTTGCAGTCTGAAAAAATGTATCAGAAG37101.0513697562579172No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA34590.9802393495676915No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG34530.9785390211209132No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG34360.9737214238550413No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT34060.9652197816211499No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT32580.9232783466006182No Hit
TTTCAGGCATAACTCTAATAATGACATATTCATATTCTAGGTTTGGGTTG29970.8493140591657622No Hit
AGATTAGGGGTTTTGCAACCTGAACACTGCCCAACAACACCAGTGTAGAA29720.8422293573041859No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT26160.7413432027953399No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC25910.7342585009337637No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTAGTGAGGAAACAAA25590.7251900825509461No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG25420.7203724852850742No Hit
CAGCCATATCAGTCTGTGGGATACGGTAGCAGATACATTGGACCACTGCT25330.7178219926149068No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA24650.6985516035514194No Hit
TTTGAAACAGAAGCAGAAGATGAATCTATCTATTTTACACCTGAACTTTA24650.6985516035514194No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG24400.691466901689843No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC23570.6679456915094099No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA22440.6359228390950852No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC21230.601632882085056No Hit
GAGACCTACATCAGGCCTTCCATCAATGCACCACTTTCTGTAGAACCATG21180.6002159417127408No Hit
GAGACCTACATCAGGCCTTCATCAATGCACCACTTTCTGTAGAACCATGT20490.5806621645747904No Hit
TTCTTCACTTGATTGGCCTTTGCTTTTGTAGAACCATGTCGTCAGTGTAC20470.5800953884258643No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA20240.5735774627132141No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG20150.5710269700430467No Hit
CAGCTGTGCCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGT18990.5381539534053328No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG18680.5293689230969782No Hit
ACACATCGTTTGGATCATGCCCTCAATCAGAAACCATTATTTCATCATTA18410.5217174450864759No Hit
GGATTGAAAACTCCTCAGCCAAATACTTCAAGGAGGATGCCTCTCTTGCC18370.5205838927886236No Hit
GCAGATGTCTTTGAGTGAGTAGAGGGGGTGGGGGAGATGTAGAACCATGT18130.5137825790015105No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT18120.5134991909270474No Hit
TGGATTTACACACTAACCTGGTAGAAATGCGACTAAAGACTGCATTGAAG18060.5117988624802691No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG17570.4979128468315796No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTTGGTGCCATTTATCGTTTTTG16510.4678737109384963No Hit
AGGAAATAATGAGCGAACCCCACAAGCAGCAAGTCATCTATCACCATGTA15570.4412352319389696No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT15000.4250821116945757No Hit
CTGGAGTGTGTGGAGGAATTACATTCACCTCTTCATCAAGGTTACTTTTT14880.42168145480101904No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG14490.41062931989696005No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT14410.4083622153012557No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT14040.3978768565461228No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA13910.39419281157810315No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA13710.3885250500888422No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCC13630.38625794549313774No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC12660.35876930227022186No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTAGAACCATGTCGTCAGT12330.3494174958129412No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT11370.32221224066448834No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT11020.3122936580582816No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTATTTTCTCCATCTGGGCTC10460.29642392588835076No Hit
TGGTGGTACATCAGGGAGGTGTAGAACCATGTCGTCAGTGTAGATCTCGG10070.2853717909842918No Hit
GGTTGACCATCAAATATTCCTTCTCCCAAGCAGATTCAAGGTGATCAGTT10040.28452162676090265No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA9870.2797040294950308No Hit
TCATTTACAGTTTGTGGGTATGCTACCGTAACCTCATCAGAATGGTAGGA9270.2627007450272478No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTGGGAGCC8550.24229680366590814No Hit
TTAAATGGAGTCATCTGAGGAGAACCATGTCGTCAGTGTAGCAGTTCTTT8200.23237822105970138No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA7770.22019253385779017No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA7720.21877559348547496No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT7510.21282444392175087No Hit
GAGACCTACATCAGGCCTTCATCCTACTCATGGAGATGTGTAGAACCATG7440.21084072740050952No Hit
GAGACCTACATCAGGCCTTCTTCCTACTCATGGAGATGTGTAGAACCATG7250.20545635398571158No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA7210.20432280168785938No Hit
TGCACGTACCACATTTAATTACCTTCTTGGTCTTCTCTTGGTATTAGTGC6950.19695471175182008No Hit
CAACTGTCAGTCTGCCATTCTTTTACGGTAGCAGACCTTTGTGCTTTTAT6910.19582115945396783No Hit
GCAGATGTCTTTGAGTGAGTAGAAGGGGTGGGGGAGATGTAGAACCATGT6290.17825109883725873No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT5850.1657820235608845No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT5560.15756376940145603No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT5200.14736179872078622No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC5070.1436777537527666No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT5000.1416940372315252No Hit
AACATCTGAGGGGTTCTGGCATTTTCAGCAGGCTGTAGAACCATGTCGTC4980.14112726108259913No Hit
CAAAAATAACTTGGAAAACGATGATAGGTGAAGCCTGTTCTTTTCCCTGT4760.134892723444412No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT4650.13177545462531845No Hit
CGGGAGGTGAGAGTTTAATAAAATAGTCAAGGACACATAGCATTAACTGA4550.12894157388068794No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT4470.12667446928498355No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG4310.12214026009357475No Hit
ATGACATGCTTCTTGAGCTTTCGGTGTTCTGAAACAACTGTAGAACCATG4270.12100670779572253No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC4250.12043993164679644No Hit
AGCAATTTCAACAGTCTAATCAATGTCGTCAGTGTACTTCTAGGCACATG4220.1195897674234073No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT4110.11647249860431373No Hit
GTTTCCTTCTTCCAAGCAGTACGGTCCTGTTTTATTTCACAAATCACACC4100.11618911052985069No Hit
ATGTGTCCCAGCTAAATGTGGTCAGTGTGAGAATGTCCAGTTTTCGTCCT4010.11363861785968322No Hit
AACATCTGAGGGGTTCAGTCATTTTCAGCAGGCTGTAGAACCATGTCGTC4000.11335522978522018No Hit
TGGCCATAGGTTCCTTCTCCAATAATCCCAATAATGTCAAACTTGTCCAC3990.11307184171075713No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACATAGAGAAATGTAG3990.11307184171075713No Hit
TTCTTCACTTGATTGGCCTTTGCTTTTGTAGAACCATGTCGTCAGTGTAG3970.11250506556183104No Hit
ATGACATGCTTCTTGAGCTTTTCGGTGTTCTGAAACAACTGTAGAACCAT3770.10683730407257003No Hit
TATGGTCCTCAGAAAAGTGCACCAGATCATCTTCCATTTGTAATACTTCT3750.10627052792364393No Hit
CCTTTAGGGTCTTTGCCCATTGATGGCTAAAACTGGTGATTTCACTAGTA3730.1057037517747178No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG3690.10457019947686562No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG3680.10428681140240256No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC3660.10372003525347646No Hit
TGGGTAATGAGAACTCTGTCAGACTCTTGTAGGCCCCCGATGTAGAACCA3650.10343664717901342No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT3620.10258648295562425No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA3560.10088615450884596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)