FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330417
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA181535.493966714787677No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG127623.862392068204723No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC116953.539466794989362No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC116133.514649669962502No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT99653.015885986495852No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA84762.5652433137520165No Hit
GACCTTTTCCTCTGCCCTTATCATCGCTTTTCAGTGATGGAGGAAATGTT73152.2138691411156204No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG71462.1627216517309944No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA55831.6896830368897484No Hit
TGTTAATAAAAATAAAACTTAACAATTTTCCCCTTTTTTTACCCCCAGTG47101.425471449713544No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG44491.3464803566402455No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAA38721.171852537853682No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA38221.156720144544621No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC37741.1421930469679222No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA37731.141890399101741No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT37721.1415877512355599No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT37671.1400745119046538No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT37211.1261527100603177No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT37041.121007696335237No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC36871.115862682610156No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC36411.1019408807658202No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAACG35921.0871111353229403No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA34061.0308186322132336No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAACGC33131.0026723806583802No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT30950.9366951458308743No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC29930.9058250634803899No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT29700.8988641625582219No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC29480.8922059095022351No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT28610.865875545144469No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT27000.8171492386892927No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG26730.8089777463023997No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC26170.7920294657962514No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC25120.7602514398472234No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAACG23490.7109198376596846No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAACGCAG22950.6945768528858988No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAACGCAGG22620.6845894733019185No Hit
GCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAA22030.6667332491972265No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAC22020.6664306013310454No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT21540.6519035037543468No Hit
ATCATGATAAAACTCTTCAGACTGATTCTATAGACAGGTATTGCACATGG20980.6349552232481984No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG20800.6295075616569366No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA20620.6240599000656746No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG20530.6213360692700436No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG20300.6143751683478755No Hit
GAGGAAGAAAAGGAAGCAGCAAAATATGTGGAGGCCCAACAAAAGAGACT19880.6016639579682643No Hit
TGAACTTATATATTTGACACAACCCAGCAGTTCGTAAGTAGTTCACAGAA19850.6007560143697207No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAACGCA18960.5738203542795922No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG18920.5726097628148673No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC18900.5720044670825047No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC17810.5390158496687519No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT17670.5347787795422148No Hit
GGCATCTGTATATTTATTTTAAACACTGCTAATAATCTTTGTCTTTTTTG17410.5269099350215032No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC15550.47061743191179634No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT15100.4569982779336414No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA14990.453669151405648No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTAAC14830.4488267855467485No Hit
CCAGCTCACAAGAGAAGAAAATACTGCTATACGTACTCCAGAACATTTAA14190.42945732211115045No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA12720.3849680857825112No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT12580.3807310156559741No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA12430.3761912976632558No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAACGCAGGAGTATC11790.3568218342276578No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAACGCAG11640.3522821162349395No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAATTCTTTAACTTACTAAAA11600.3510715247702146No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA11580.35046622903785213No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAAC11480.34743975037603997No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT11420.34562386317895266No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTGA11370.3441106238480466No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG11100.3359391314611536No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGTTGAGGTTTCAATGAATG11080.33533383572879116No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA10950.3313994134684353No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT10910.33018882200371047No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAACGC10810.32716234334189825No Hit
TCAGATATAAAAGAAGAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTA10550.31929349882118657No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC10420.31535907656083073No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTAACGCAG10240.30991141496956875No Hit
TTTTTGTCCAGCCAGCAAATGATCAGCCTTGCAAAGACCAAGTCTCTGCT9840.29780550032232No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTAACGCA9000.27238307956309754No Hit
AATAGTGTAAAGCAGCATATAAAAATGACTCTAGGTAGACCAAGTCTCTG8890.26905395303510415No Hit
CAAAGAGACTCACCTAATTTTCTTACATTTTGTTTTTGTAGGTAATTTTA8840.2675407137041981No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA8770.2654221786409295No Hit
GCTATTCCTACCATTCTGATGAGGTAGACCAAGTCTCTGCTACCGTAACG8750.26481688290856703No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAACGCA8650.26179040424675487No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA8600.26027716491584874No Hit
TTAACTACAATTCCCAGAAGAGCCCAATTCGAGACCAAGTCTCTGCTACC8160.2469606588038751No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC7780.23546003988898878No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT7580.22940708256536435No Hit
TCTCCATATCTCTCTCAATTTCAACAAGACAAACAACAGTTGGTATTAGG7230.21881440724902168No Hit
ATGGTTGATAGAGTGGTAGACCTTGCAGACCAAGTCTCTGCTACCGTAAC7140.2160905764533907No Hit
GAGTTTGATGCACAACTTCAAGGCAATCTCAAGACCAAGTCTCTGCTACC6900.20882702766504146No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAGACCAAGTCTCTGCTACCGT6680.2021687746090546No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA6640.20095818314432973No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTTATACTTTAACAGGATTTG6520.19732640875015509No Hit
GGGAAAAGAACAGGCTTCACCTAAAAACGTAGACCAAGTCTCTGCTACCG6430.19460257795452413No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG6370.19278669075743682No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC6240.18885226849708095No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC5870.17765429744837583No Hit
AAAGTGAGGCACTCCAGTTTTATTTAAGTATTCCCAATATAAGTAGACCA5850.1770490017160134No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAACGCAGGA5840.17674635384983217No Hit
AGCAATCTGCTTGTGTGTGCGTGAGACCAAGTCTCTGCTACCGTAACGCA5730.17341722732183878No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTAAC5650.17099604439238902No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC5640.1706933965262078No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG5510.16675897426585196No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG5340.1616139605407712No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA5130.1552583553509656No Hit
TGCCTTGTTTCTTGCTTTCGTATAAGCCCTCACAACAACCAACACTTTAA5130.1552583553509656No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG4940.14950804589352243No Hit
TTCAGGAAGAAGAACGATTATCCAGAAAGACCAAGTCTCTGCTACCGTAA4900.14829745442879755No Hit
GTAGACAACATAAAGCCTCATATGTTAATTGCTGCAGACCAAGTCTCTGC4900.14829745442879755No Hit
GCTCTTCCCCCCAAAAAATAAATCAATAAAAGTTTTCAGACCAAGTCTCT4860.14708686296407267No Hit
TGTTTTTGTAGGTAATTTTAACCAATCAGATGACAACAAAGATTGATAGA4850.14678421509789144No Hit
GAAGTTCATCCTGTTTTAAGTCACACTTGTGATTTGTTAAATTTTTTAAC4790.14496832790080413No Hit
TGCATTCTGAAAAAGCCAGAATTTACAGCTCTCCAGACCAAGTCTCTGCT4770.1443630321684417No Hit
AGGTTCAACTACGCGTGTAAATTCTACTGCAAATGCAGAGACACAAGCAA4750.14375773643607925No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGATTTCATTTCAGTTAATGCT4670.14133655350662952No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA4650.14073125777426704No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT4570.1383100748448173No Hit
ATTTGTTGCAGGTTTTGGTTTTAATGGCTTGTGGCGGTTTGCAGGACCGT4340.13134917392264925No Hit
CGGGAGCCCACTTCATTAGTACTGGAGACCAAGTCTCTGCTACCGTAACG4310.1304412303241056No Hit
TCAAAGGGCCCCAGACTGCAGACCAAGTCTCTGCTACCGTAACGCAGGAG4180.12650680806374973No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA4120.12469092086666243No Hit
CATTGGTGCTAAAAGTTTCTTGGATCACATTTTAGACCAAGTCTCTGCTA4100.12408562513429998No Hit
AGATGAGTCATATTTGTGGGTTTTCATACAGCTTCCCTCTTTAAGATGTC4080.12348032940193755No Hit
GCTCTTCACCCTGCAAAAATAAATCAATAAAAGTTTTCAGACCAAGTCTC4000.12105914647248779No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC3970.12015120287394415No Hit
AACTGAGCGACCTCAGAGACCAAGTCTCTGCTACCGTAACGCAGGAGTAT3860.11682207634595072No Hit
TGTGTCATGTAATCAAATAGTAGATGTGACACTGACGACATTGATTAGAC3840.11621678061358828No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC3760.11379559768413854No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA3670.11107176688850756No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA3660.11076911902232633No Hit
GGGAAAATTATGCTTACTAATGTGGTTTTAATTTCATCATGTTTCATATA3600.10895323182523901No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA3570.10804528822669535No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT3550.10743999249433292No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT3420.10350557023397705No Hit
AGTTTTTGTGTTGATGAAGAGGAGTATGATTCTATAGACCAAGTCTCTGC3330.10078173943834608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)