FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335711
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA152534.543491276723135No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC100642.9978165743749834No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG95302.8387511877775826No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC84352.512577782676171No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG84202.508109653839165No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT68182.030913494046963No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA64211.9126570174942137No Hit
GACCTTTTCCTCTGCCCTTATCATCGCTTTTCAGTGATGGAGGAAATGTT58911.7547831319200144No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA52861.574568602160787No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG51561.5358448189067382No Hit
TGTTAATAAAAATAAAACTTAACAATTTTCCCCTTTTTTTACCCCCAGTG50191.4950359088620868No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT47141.404183955842972No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC44491.3252470130558724No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA41241.2284375549207502No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA40971.2203949230141402No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC39811.1858413933412966No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT38461.1456282338082457No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA35791.0660955405095454No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGAAAATGAAATACTTTGA34751.0351165139063063No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAACAA32890.9797117163274364No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT32760.9758393380020315No Hit
TGAACTTATATATTTGACACAACCCAGCAGTTCGTAAGTAGTTCACAGAA32450.9666052050722198No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAA31170.9284771723297718No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA30610.9117961580049507No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT30570.9106046569817492No Hit
ATCATGATAAAACTCTTCAGACTGATTCTATAGACAGGTATTGCACATGG30420.9061365281447434No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT30350.9040514013541409No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT29930.8915406406105251No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC28910.8611573645188868No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG28090.836731593543256No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAACA27490.8188590781952334No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG26850.7997950618240093No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT25210.7509435198727478No Hit
GCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAA24100.717879366478906No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC23970.714006988153501No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC23920.7125176118744991No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC23710.7062622315026913No Hit
GAGGAAGAAAAGGAAGCAGCAAAATATGTGGAGGCCCAACAAAAGAGACT23320.6946450965264767No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT22860.6809428347596593No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA22430.6681341987602432No Hit
GGCATCTGTATATTTATTTTAAACACTGCTAATAATCTTTGTCTTTTTTG22160.660091566853633No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAACAACGA21530.6413254257382093No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAC21310.634772170110601No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT20040.5969420126239533No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAACA19100.568941738578718No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC18780.559409730393106No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTGA18000.5361754604406767No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT17820.5308137058362699No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAACAACG17300.5153241925346503No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC16460.49030267104741876No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG16200.482557914396609No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAACAAC16110.4798770370944056No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG16050.47808978555960335No Hit
CCAGCTCACAAGAGAAGAAAATACTGCTATACGTACTCCAGAACATTTAA15960.4754089082573999No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA15670.46677052583918904No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT15580.46408964853698564No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG15320.4563448918861759No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT15060.4486001352353662No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA14950.44532350742156196No Hit
CAAAGAGACTCACCTAATTTTCTTACATTTTGTTTTTGTAGGTAATTTTA14890.4435362558867597No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG14770.4399617528171552No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA14120.4205998611901308No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA13900.41404660556252254No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT13410.39945071802830406No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA13320.3967698407261007No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC13100.39021658509849244No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTTATACTTTAACAGGATTTG12990.38693995728468833No Hit
TCTCCATATCTCTCTCAATTTCAACAAGACAAACAACAGTTGGTATTAGG12250.3648971883554605No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT12140.36162056054165637No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTAAC11980.35685455644885034No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGGGCAAAATGAGATATTGTGATAGA11780.3508970513328429No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAATTCTTTAACTTACTAAAA11660.34732254826323833No Hit
GTCACTCATTGACAAAAGAGGTACTGTAGAGCCATGTTGTGACCCTAAAT11320.33719478956602555No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT11050.3291521576594154No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC10890.3243861535666094No Hit
ATTTGTTGCAGGTTTTGGTTTTAATGGCTTGTGGCGGTTTGCAGGACCGT10570.3148541453809973No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAACAACG10500.3127690185903947No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA10280.30621576296278646No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAAC10080.30025825784677895No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG9900.29489650324237215No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAACAACGAACAATC9800.2919177506843684No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC9710.289236873382165No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA9410.28030061570815373No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTAACAACG9320.27761973840595033No Hit
TGTTTTTGTAGGTAATTTTAACCAATCAGATGACAACAAAGATTGATAGA9300.2770239878943496No Hit
TTTTTGTCCAGCCAGCAAATGATCAGCCTTGCAAAGACCAAGTCTCTGCT9140.2722579838015436No Hit
AGGTTCAACTACGCGTGTAAATTCTACTGCAAATGCAGAGACACAAGCAA8870.26421535189493345No Hit
GAAGTTCATCCTGTTTTAAGTCACACTTGTGATTTGTTAAATTTTTTAAC8610.25647059524412363No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAACAA8460.25200246640711804No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA8400.25021521487231574No Hit
TGCCTTGTTTCTTGCTTTCGTATAAGCCCTCACAACAACCAACACTTTAA8280.24664071180271127No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA8160.24306620873310675No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA8010.23859807989610113No Hit
TCAGATATAAAAGAAGAGGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTA7980.23770445412869995No Hit
GCTATTCCTACCATTCTGATGAGGTAGACCAAGTCTCTGCTACCGTAACA7890.2350235768264966No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTAACAAC7630.22727882017568682No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAACAAC7250.21595956045527254No Hit
GTTCAGCCCAGTTTGAAGCAAATGCTTTTGAAGCACCACTTACATTTGCA7130.212385057385668No Hit
TAAAGCATGAGAAAACTACTATGACTTCTAAATTACGAAAAAATGTTAAA6940.2067254275254609No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT6750.20106579766525373No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGATTTCATTTCAGTTAATGCT6750.20106579766525373No Hit
AATAGTGTAAAGCAGCATATAAAAATGACTCTAGGTAGACCAAGTCTCTG6560.1954061678050466No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG6520.1942146667818451No Hit
TTAACTACAATTCCCAGAAGAGCCCAATTCGAGACCAAGTCTCTGCTACC6520.1942146667818451No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG6270.1867677853868357No Hit
ATGGTTGATAGAGTGGTAGACCTTGCAGACCAAGTCTCTGCTACCGTAAC6230.1855762843636342No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA6090.18140603078242895No Hit
GGGAAAAGAACAGGCTTCACCTAAAAACGTAGACCAAGTCTCTGCTACCG5560.16561864222500902No Hit
TCCAAAGTAATATCCAATGTAAAAGATGCAGACCAAGTCTCTGCTACCGT5320.1584696360858No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG5290.15757601031839885No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG5210.15519300827199584No Hit
CTGACACTTACAACAGTCAAGTTCTGATAGAAGCTACAGTAATAGCACTA5200.15489513301619548No Hit
GTAACCAGGCATTATGATCTTTGAAAATTTTTATTTGCGTATCTTTAATT5160.15370363199299397No Hit
TTTGCTAATAGATGCCTAAGCCCAGAAAGGGTGCTTCTTCAACTAAAATA5120.1525121309697925No Hit
GGCCAGGGGTTGTGCTTTTTAAATTTCAATTTTATTTTTGCTAAGTATTT5000.14893762790018797No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC4980.1483418773885872No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC4980.1483418773885872No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAACAACGAA4950.14744825162118608No Hit
GGGAAAATTATGCTTACTAATGTGGTTTTAATTTCATCATGTTTCATATA4860.1447673743189827No Hit
GTAGACAACATAAAGCCTCATATGTTAATTGCTGCAGACCAAGTCTCTGC4670.13910774445877555No Hit
GAGTTTGATGCACAACTTCAAGGCAATCTCAAGACCAAGTCTCTGCTACC4630.13791624343557407No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA4520.1346396156217699No Hit
AGCAATCTGCTTGTGTGTGCGTGAGACCAAGTCTCTGCTACCGTAACAAC4340.12927786101736316No Hit
AAAGTGAGGCACTCCAGTTTTATTTAAGTATTCCCAATATAAGTAGACCA4260.12689485897096014No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTAAC4170.12421398166875676No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT3970.11825647655274923No Hit
CGGGAGCCCACTTCATTAGTACTGGAGACCAAGTCTCTGCTACCGTAACA3950.11766072604114848No Hit
ATTTAACACCTAGCCAAAAGGCAGAAATTACAGAACTTTCTACTATATTA3850.11468197348314473No Hit
TCAAAGGGCCCCAGACTGCAGACCAAGTCTCTGCTACCGTAACAACGAAC3810.11349047245994323No Hit
TGATAGAATCTGATGAAGAATGTGCTGCCGTAAGATAATGACAGAAATTC3650.10872446836713721No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC3470.10336271376273044No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG3460.10306483850693007No Hit
TGAAGAAGGAGATGGTGTGGAATTGATGGAAGCCAAATCACAGTTATTTC3370.10038396120472669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)