FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335711
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT149674.458298953564226No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG101383.019859343304211No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT95942.8578152041488067No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG78832.3481506414743634No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA74342.2144046516199944No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC66121.9695511913520856No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC62761.869465105403159No Hit
AGAAAGAAATATATGGTAAGTTTCAAGAATACATCATACCTGTATAGGGT53331.5885687391834047No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG48381.4411204875622186No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT47161.4047797063545728No Hit
ATTACTAAGTCATAAAAATAAACCAGGTAGAATATTTACCTTCACAAACT47021.4006094527733677No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA45461.354140912868509No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG42561.2677570886864No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT41991.2507781991057785No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA39711.1828626407832927No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA39611.179883888225289No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA37451.1155428329724077No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT36411.0845638063691687No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTAAGAACACAGAGGAGAATTTA35051.0440527715803176No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT33741.0050311130704683No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC31900.9502220660031991No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA31380.9347325527015795No Hit
TATGAAGTAGCCTGCTATAATCGATCAAACTAACCCTAACTATCAAGATG30550.9100089064701484No Hit
GTTATAAATGATATCTAAAGGTCACTAAGCTAAAGACTATATAATCTACC30040.8948172684243293No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG29530.87962563037851No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG29070.8659233686116927No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA28040.8352422172642541No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG28040.8352422172642541No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA27700.8251144585670414No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG26540.7905609288941977No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT26450.7878800515919943No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC26440.787582176336194No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT25660.7643479063837646No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT24350.7253262478739153No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG24000.7149006139209022No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG23000.6851130883408646No Hit
CATATTAGAAATAACAATGTGTACCATATAACTAATTTTACCTTCATGTT22750.6776662069458552No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC21580.6428148020172112No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG21170.6306019165293959No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG20450.6091548981117687No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT20010.5960483868565523No Hit
TGGCTTTGCATAATATGGCTTTAAAGTCAGTAAGACATGGTATATCAGCA19890.5924738837869477No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA19640.5850270023919383No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA19410.5781758715085297No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT17550.5227710739296597No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT17250.5138348162556485No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT16740.4986431782098293No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT16490.49119629681481985No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC16380.48791966900101574No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT16180.4819621638850083No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC16160.48136641337340746No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT15780.4700471536529932No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT15560.46349389802538493No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG15230.45366401458397254No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT15070.4488980104911665No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT14620.43549362398014957No Hit
ACTTGCTTTCCACTTGCTGTACTAAATCCAGAGAAAGCAGATGAATTTAC14580.43430212295694803No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA14510.43221699616634546No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA13490.40183372007470713No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG13320.3967698407261007No Hit
ACTAAAATGTTTATCTGATTAATTACCCACCTAATGCAGGAACAAGCAAG13310.3964719654703004No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT13160.39200383663329474No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAACTGATTACCTTGCTT13060.38902508407529096No Hit
CTGAGTAAGTGGCAAGAAAATTACCTGCGTGCTGCATCAAAGTTGCTTTT13040.3884293335636902No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG12250.3648971883554605No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC12150.3619184357974567No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA12110.3607269347742552No Hit
ATCACAATATCTCATTTTGCCCTCTTTCTTCACAGGTTTTGAAGGAATGT11880.35387580389084655No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT11840.35268430286764507No Hit
ACAACTGTTTCATATACTTCATCTTCTAGGACATTTGGCATTGACTTTCC11450.34106716789143043No Hit
AAGATTTAGGGTCACAACATGGCTCTACAGTACCTCTTTTGTCAATGAGT10810.32200315152020637No Hit
AGAAAAAGTTTCAGTTTTACCAATTTCCATTTTTACGTTTTTAGGTGAAG10650.31723714742740033No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT10420.31038601654399167No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT10330.30770513924178833No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA10320.30740726398598794No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT10250.3053221371953853No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAGTGCAAACAATACATCATACTT10130.30174763412578076No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG10010.2981731310561763No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC9930.2957901290097733No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA9830.2928113764517695No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAACAGAAACCTTCATTT9790.29161987542856804No Hit
TTGTGAAGAGAACATGATTGGCAATACTCTGAAGTATCTACACAATAAAA9740.2901304991495662No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT9620.28655599607996163No Hit
ACCTGTATAGGGTATGCTCTTTGAATAATTACATCAACACAACCAACATT9590.2856623703125605No Hit
TTGCAAGGCTGATCATTTGCTGGCTGGACAAAAATGTAGAACCATGTCGT9180.2734494848247451No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT8560.25498121896512177No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTAGGCTGCAGCGACTTCGAACT8560.25498121896512177No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA8380.24961946436071503No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG8370.24932158910491464No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC8260.2460449612911105No Hit
CCAACTGGCCAAGACCTCTTCTTTTATATCTGATGTAGAACCATGTCGTC8230.24515133552370938No Hit
CGATAAAAGTGAACCTATTAATCTCTATAGTCTTTTTGTTTATAAAGGAT8060.24008745617510296No Hit
ACCTCATCAGAATGGTAGGAATAGCTGTAGAACCATGTCGTCAGTGTAGA7960.23710870361709924No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA7730.23025757273369057No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC7710.2296618222220898No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC7650.2278745706872876No Hit
AATCAAATGAAAGAGATAAGAAAAACTGTGGTAAACTAAAATGTTTATCT7590.22608731915248534No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC7290.21715106147847402No Hit
GAAAAATAACATTCTGTGAACTACTTACGAACTGCTGGGTTGTGTCAAAT7030.20940630482766429No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC6620.19719341933984885No Hit
ACCTAGAGTCATTTTTATATGCTGCTTTACACTATTTGTAGAACCATGTC6600.19659766882824814No Hit
CTGAATGAGAAAGTAAAAGGGGCATAAATTAAATACTTACTATATGGTTC6580.19600191831664737No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG6560.1954061678050466No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA6540.19481041729344584No Hit
CGAATTGGGCTCTTCTGGGAATTGTAGTTAATGTAGAACCATGTCGTCAG6470.1927252905028432No Hit
GCAAGGTCTACCACTCTATCAACCATTGTAGAACCATGTCGTCAGTGTAG6290.18736353589843646No Hit
CGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA6180.18408690808463232No Hit
AAACACAGAAGGAATCGTCATCTATAAAACTATATGTACTATTTACAAAA6050.18021452975922744No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT5850.17425702464321993No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCTGTAGAACCATGTCGTCAGT5590.16651226799241015No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT5420.16144838864380373No Hit
GCATCTTTTACATTGGATATTACTTTGGATGTAGAACCATGTCGTCAGTG5360.1596611371090015No Hit
CGCGTTGCCTTTGTTTCTTCTTAATTCGCATATCCTGTATATCTCTGGCA5260.15668238455099773No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT5140.15310788148139323No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG4900.1459588753421842No Hit
GTCACTGACAACTGGCTTGTGCAACATTTTGACATGGAAGTCACAGACTA4850.14446949906318232No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG4840.14417162380738194No Hit
AGTGACGTGTCCTCTGTTCCTTCTCTTTCTTCACAGGTTTTGAAGGAAGA4720.14059712073777741No Hit
GCAGCAATTAACATATGAGGCTTTATGTTGTCTACTGTAGAACCATGTCG4710.14029924548197706No Hit
TGAGATTGCCTTGAAGTTGTGCATCAAACTCTGTAGAACCATGTCGTCAG4650.1385119939471748No Hit
TGAAAGAAAGTAACATTCAACACTGATACCCAAAATGAATGCACTCATTT4630.13791624343557407No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC4500.13404386511016916No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA4470.13315023934276804No Hit
CGAAAGAAATATATGGTAAGTTTCAAGAATACATCATACCTGTATAGGGT4420.13166086306376615No Hit
CACGCACACACAAGCAGATTGCTTGTAGAACCATGTCGTCAGTGTAGATC4390.13076723729636502No Hit
TGTGATTATTCTGGAGAGGAAGATTAAGAGGACAAGCAGATTCAAGGTGA4340.12927786101736316No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA4320.1286821105057624No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG4290.12778848473836127No Hit
ACTTATATTGGGAATACTTAAATAAAACTGGAGTGCCTCACTTTTGTAGA4160.12391610641295638No Hit
CCAGTACTAATGAAGTGGGCTCCCGTGTAGAACCATGTCGTCAGTGTAGA3990.11885222706434997No Hit
CGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG3880.11557559925054585No Hit
GCAGTCTGGGGCCCTTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGG3830.11408622297154397No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA3790.11289472194834246No Hit
CATCTGGTTTTCAGGCACTTCAAATGTACTCTTCTGCAATATGTAGCTTG3750.11170322092514096No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG3470.10336271376273044No Hit
TCAACAGTGGAATTTCTGTCATTATCTTACGGCAGCACATTCTTCATCAG3420.10187333748372858No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA3390.10097971171632743No Hit
TTCTGGATAATCGTTCTTCTTCCTGAATGTAGAACCATGTCGTCAGTGTA3370.10038396120472669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)