FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55512
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC1153820.784695201037614No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC634111.422755440265169No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG43507.836143536532641No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC40377.272301484363741No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT28675.164649084882548No Hit
GCGTGGACAACCCCCACCTGATGATCCTTCCCTACCCACCCTGGTAGCTG17013.06420233463035No Hit
CTGAGGGAAGCAGCAATTCTCTGCTACCGTACCAAGAGGGGGAAGAGGGA10341.862660325695345No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA7791.4033001873468798No Hit
CCAGCTCACAAGAGAAGAAAAGCCCCAGAATCTCTTATGTTAAGACCAAG6751.2159533073929962No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATCA6031.0862516212710764No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA5641.01599654128837No Hit
TTTCGCTTCCCACAGGTCTCCCTGAGCCTCCTGGACTATGTCCGGAGACC5460.9835711197578901No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA5210.9385358120766681No Hit
AGAAGACCAGGAAAGAACGGGTTCAGTGCCATTGACACAGACCAAGTCTC5040.9079118028534372No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATC4880.879089205937455No Hit
ACTTTCCTCTGGCAATTGGACATTTGGAGCCCTTTGAGGCCTTTGCATCC4460.8034298890330018No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA3960.7133592736705577No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC3930.7079550367488111No Hit
TATTTGTTCAGGGCTCTGTGTGACCCAGAGCAACTGTGCATGTAGACCAA3790.6827352644473267No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATCATAT3730.6719267906038334No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC3670.6611183167603402No Hit
GGTCCAGCAGCCCTTTCCCCAGCTTCCCTCTTTAAGATGTCTCTAGACCA3650.6575154921458424No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA3620.6521112552240957No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATCATATC3530.6358985444588557No Hit
TCTATGCTTGTTTCCCGACTGTGTGTGGCAGAGCAAAGGGCCCCAGACTG3010.5422251044819139No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC2780.5007926214151895No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC2600.46836719988470965No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT2490.44855166450497186No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATCAT2480.446750252197723No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATCA2450.44134601527597633No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATCAT2290.4125234183599943No Hit
CCCAAGATTACTGGCATCTTCCTGCCAAGAAATGGAGCATGAGGAGGAGC2070.3728923476005188No Hit
CCAGAGACCAGACTCCAGAGCCGATATTGACAAGAAGATAGGGGAAAATG2030.3656866983715233No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATCATAT1990.35848104914252776No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG1790.3224528029975501No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA1540.277417495316328No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC1500.27021184608733245No Hit
AGAGGAACGTTACACACCAGCAGGGGAGCACGGAGCCCACTTCATTAGTA1470.2648076091655858No Hit
TTTCCTCTAAAATGTTCCTCTGTTGTACGGTAGCAGCAGGACTCAGAGAC1340.24138924917135032No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT1300.23418359994235483No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATCATA1270.22877936302060814No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT1260.2269779507133593No Hit
AGGTGTGTGTGTGGCTTCAAAGAGCATACCCTATACAGGTAGACCAAGTC1250.22517653840611038No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG1250.22517653840611038No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA1160.20896382764087046No Hit
TCTTGCAAAAACATCCACTCTGTTCCCTTCTGTTCCAATGAACTTTAACA1070.19275111687563048No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATCATA1070.19275111687563048No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT960.17293558149589278No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT910.16392851995964838No Hit
TGGTGATTTGCTGATATACCATGTCTCTGCTACCGTATGGTGGTGGTAAT890.16032569534515062No Hit
GCCGGAACAGCCCCAGGAAGCCAAATCACAGTTATTTCTTAAGAGACCAA890.16032569534515062No Hit
GAGAAAGCCCCCCTACTGCACACTGACGACATGGTTCTACAGAGAAAGCC880.15852428303790173No Hit
GTTAGAAGGCTGGCTCCCAGATGGCATCTCTGGACCACCCAAGGGGGGGA840.1513186338089062No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT800.14411298457991065No Hit
AACTGAGCGACCTCAGAGCCCTGGTGGGGTAGACACTGGAGGGAATCTCC780.14051015996541288No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA760.13690733535091512No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTATCATATCG740.13330451073641736No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT730.13150309842916846No Hit
GGGGTCAGAGGCAAGCAGCAGTGTTCTGCAATCCTTTGTGGGGGTTGTTG720.12970168612191957No Hit
CTGAGGGAAGCAGCAATTCTCTGCTACCGTACCAAGAGGGGGAAGAGGGG720.12970168612191957No Hit
ATAGGAGCCTTGAGGGCCAAAGCTGCCCTTCCCTGTCCTTGCCAGACCAA690.12429744920017292No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAAACCAAGTCTC660.11889321227842628No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGC660.11889321227842628No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT650.11709179997117741No Hit
ACACCACTCTCTAAATCAAACACATTGGAGACCAAGTCTCTGCTACCGTA650.11709179997117741No Hit
TTCCTTCAAAACCTGTGAAGAAAGAACAGTGCAGTTTCCAGGGGAAGACC620.11168756304943075No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA600.10808473843493299No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTATC570.10268050151318635No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)