FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55512
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG1145820.6405822164577No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG610210.992217898832685No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT42937.733463035019455No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG39857.178628044386799No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT28595.150237786424556No Hit
GCAGTCTGGGGCCCTTTGTCATCAGCTACCAGGGTGGGTAGGGAAGGATC16963.055195273094106No Hit
TCCCTCTTCCCCCTCTTGGTACGGTAGCAGAGAATTGCTGCTTCCCTCAG10001.801412307248883No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT7741.3942931258106357No Hit
TAACATAAGAGATTCTGGGGCTTTTCTTCTCTTGTGAGCTGGTGTAGAAC6811.2267617812364895No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA5921.0664360858913389No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT5631.014195128981121No Hit
CCGGACATAGTCCAGGAGGCTCAGGGAGACCTGTGGGAAGCGAAATGTAG5430.9781668828361435No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT5280.9511456982274102No Hit
GTGTCAATGGCACTGAACCCGTTCTTTCCTGGTCTTCTTGTAGAACCATG5030.9061103905461881No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG4890.8808906182447038No Hit
AGGGATGCAAAGGCCTCAAAGGGCTCCAAATGTCCAATTGCCAGAGGAAA4040.7277705721285488No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT3950.7115578613633088No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG3930.7079550367488111No Hit
ACATGCACAGTTGCTCTGGGTCACACAGAGCCCTGAACAAATATGTAGAA3790.6827352644473267No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT3750.6755296152183312No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG3670.6611183167603402No Hit
AGAGACATCTTAAAGAGGGAAGCTGGGGAAAGGGCTGCTGGACCTGTAGA3660.6593169044530912No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT3580.6449056059951002No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC3490.6286928952298602No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG3230.5818561752413892No Hit
GCAGTCTGGGGCCCTTTGCTCTGCCACACACAGTCGGGAAACAAGCATAG2970.5350194552529183No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG2680.48277849834270065No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT2490.44855166450497186No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA2450.44134601527597633No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA2400.43233895373973197No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT2340.42153047989623865No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT1980.35667963683527887No Hit
AGACAGTTAATATCACTGCAGGCTTGGGCCCCTGGGCTCCTCCTCATGCT1900.34226833837728776No Hit
ACAAGTCAACAACCCCCACATTTTCCCCTATCTTCTTGTCAATATCGGCT1840.3314598645337945No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT1800.324254215304799No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT1510.27201325839458135No Hit
CCAGTACTAATGAAGTGGGCTCCGTGCTCCCCTGCTGGTGTGTAACGTTC1390.25039631070759477No Hit
GGTCTCTGAGTCCTGCTGCTACCGTACAACAGAGGAACATTTTAGAGGAA1350.24319066147859922No Hit
TTGTGAAGAGAACATGATTGGCAATACTCTGAAGTATCTACACAATAAAA1340.24138924917135032No Hit
ACCTGTATAGGGTATGCTCTTTGAAGCCACACACACACCTTGTAGAACCA1270.22877936302060814No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC1260.2269779507133593No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG1250.22517653840611038No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT1220.21977230148436375No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA1150.20716241533362154No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA1140.2053610030263727No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1080.19455252918287938No Hit
ATGTGTTAAAGTTCATTGGAACAGAAGGGAACAGAGTGGATGTTTTTGCA980.17653840611039054No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC970.17473699380314164No Hit
GAAAGCCAGGGAGTTGGCTTTCTCTGTAGAACCATGTCGTCAGTGTGCAG910.16392851995964838No Hit
CTTAAGAAATAACTGTGATTTGGCTTCCTGGGGCTGTTCCGGCTGTAGAA890.16032569534515062No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT890.16032569534515062No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC790.14231157227266178No Hit
AGGGCTTTAAAATTACCACCACCATACGGTAGCAGAGACATGGTATATCA780.14051015996541288No Hit
AGTGAGGATGAAGAGCTTCCCCCCCTTGGGTGGTCCAGAGATGCCATCTG770.138708747658164No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG760.13690733535091512No Hit
CCCCTCTTCCCCCTCTTGGTACGGTAGCAGAGAATTGCTGCTTCCCTCAG730.13150309842916846No Hit
GGCAAGGACAGGGAAGGGCAGCTTTGGCCCTCAAGGCTCCTATTGTAGAA710.1279002738146707No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT700.1260988615074218No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC690.12429744920017292No Hit
TACCTTTGAAAACGGTCCTGCAAACCAGGAGATTCCCTCCAGTGTCTACC660.11889321227842628No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT650.11709179997117741No Hit
ACAAGTCAACAACCCCCACAAAGGATTGCAGAACACTGCTGCTTGCCTCT640.11529038766392853No Hit
TCCCCTGGAAACTGCACTGTTCTTTCTTCACAGGTTTTGAAGGAATGTAG610.10988615074218187No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT600.10808473843493299No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT570.10268050151318635No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG560.10087908920593745No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)