FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65282
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC860613.182806899298427No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC745111.413559633589657No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG683510.469961091878313No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC51017.813792469593456No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA29704.549492968965412No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA26304.028675592046812No Hit
GATTCCCGCGCCCAGAGTGTTGGCAGGACGCACCTTGGCCTCGATGCCGA21653.316381238319904No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC19973.0590361814895375No Hit
TCTATGCTTGTTTCCCGACTGTGCTCTGCAGTACAGTGGGAAGAGAGGTG13872.124628534664992No Hit
GGAAAACCAGCGGAACCTCCCATCACAAGCCAGTTGTCAGTGACAGACCA13712.1001194816335285No Hit
GTTAGAAGGCTGGCTCCCAGGGTGATGTGAGGAGTAACCTTTTAATAAGA11181.7125700805735118No Hit
CAGCAGTTACCAGGGCACAGGTCAAGGCCCAGAGGCAGGGTAGAACCAGG9911.5180294721362704No Hit
TGAACTTATATATTTGACACAACCCAGCAATCCCATTACTGGGTGCATAC9181.406206917680218No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT8011.2269844673876413No Hit
TACCCTCCATGAGGCACACTGTCTATGAAGTGGGAAAAATATTAGTGTCG7391.1320118868907203No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA6180.9466621733402776No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATGT5920.9068349621641494No Hit
TACTGAATGCAAAGGACACCACAGGGCAGAACGAGCAGATCCTCGGACAC5590.8562850402867559No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA5420.8302441714408259No Hit
GGGATAAAAGCACAAAGGTCTGCCTTTGGGAATCAACTACCTTCAGAGCT4500.6893171165099109No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT4320.6617444318495145No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATGTACA4230.6479580895193162No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC4100.6280444839312521No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA3850.5897490885695904No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA3780.5790263778683251No Hit
TAAAGGAACTGTAGTCGCCCTGGTTGATAGAGTGGTAGACCTTGCAGACC3520.5391991666921969No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATG3190.48864924481480343No Hit
TGCATTCTGAAAAAGCCAGACTAAGTCTCTGCTACCGTATCAGGTTGCAA3080.47179927085567236No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATGTACAG2770.4243129806072118No Hit
CTGTATGTAGTCGGTGCTGTGACCCCGTGCCTGAGTAACCCAGAGTCTGT2640.4043993750191477No Hit
CAGCAGTTACCAGGGCACAGGCCCTGCAACAGGTGTACAACGAGATGGAG2630.40286755920468126No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC2620.4013357433902147No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC2560.39214484850341597No Hit
GTCTCGGAGGCTGGCATCTAAACTACAGACAACCCAAGGGTCATCTCAAC2520.3860175852455501No Hit
GCCGGAACAGCCCCAGCAGTCCCCGGTGTGTGATGTTCCCCTTCCCTGTC2440.3737630587298183No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA2440.3737630587298183No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC2380.3645721638430195No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATGTA2310.35384945314175426No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT2160.33087221592475724No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATGT2130.3262767684813578No Hit
TTCAGGTGTATTTTGGAGGCCAAGTCTCTGCTACCGTAAGGTTCTGAATT2120.32474495266689135No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT1780.2726632149750314No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACGAGA1750.26806776753163203No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATGTA1660.25428142520143376No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT1640.25121779357250085No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATGAGAC1620.24815416194356793No Hit
CTGCACAGTGAATCCAAAAGAAAGTACCGAGGCACCTTCTCCCCTTGATC1620.24815416194356793No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATGTACA1400.2144542140253056No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG1400.2144542140253056No Hit
TGAACTTATATATTTGACACAACCCAGCCCTTAACAATGGTTTCCTGGAC1340.2052633191385068No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC1300.19913605588064093No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA1250.19147697680830855No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATGTAC1070.16390429214791213No Hit
TTCAGGTGTATTTTGGAGACCAAGTCTCTGCTACCGTATGTACAGCGAAT1060.16237247633344568No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA1050.1608406605189792No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT1050.1608406605189792No Hit
GCCGGAACAGCCCCAGCTCACTCATCCTCCCTTCCCTGTCCTTGCCAGAC1040.15930884470451273No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT960.14705431818878098No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATGTAC950.14552250237431452No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG940.14399068655984804No Hit
TACTGAATGCAAAGGACACCACATATGGACCACATTTAGCTGGGACACAT800.12254526515731749No Hit
AGATGATACTGCTGCCCTCGCTCCATGAGTCCCACGACCCCCTCAGACCA750.11488618608498515No Hit
AGATGATACTGCTGCCCTCGCCACCTTGCTCAGGTACATCCACAGACATA720.11029073864158574No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT710.10875892282711927No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)