FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65282
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG803212.303544621794675No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG739811.332373395422934No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT64929.944548267516314No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA50687.763242547716062No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT29274.483624888943353No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT26194.0118256180876815No Hit
ATGAGGTACTCGTCGGCATCGAGGCCAAGGTGCGTCCTGCCAACACTCTG19953.0559725498606047No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA19883.0452498391593394No Hit
GTCACTGACAACTGGCTTGTGATGGGAGGTTCCGCTGGTTTTCCTGTAGA13592.0817376918599306No Hit
CTACCAGTGCCAGGAGCGGACCTGACGAGGTGGTCACCTCTCTTCCCACT13562.0771422444165313No Hit
TATTAAAAGGTTACTCCTCACATCACCCTGGGAGCCAGCCTTCTAACTGT11001.6849973959131153No Hit
GCTGCCCCCACCATGAGGACCCTTTGCCCTGGTTCTACCCTGCCTCTGGG10341.5838975521583285No Hit
TCATTTACAGTTTGTGGGTATGCACCCAGTAATGGGATTGCTGGGTTGTG8371.2821298367084342No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA7781.1917527036549125No Hit
GGCGACACTAATATTTTTCCCACTTCATAGACAGTGTGCCTCATGGAGGG7221.1059710180447904No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT6140.9405349100824116No Hit
GGTCTTTGCCTGCTGAGAGTGTCCGAGGATCTGCTCGTTCTGCCCTGTGG5610.859348671915689No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT5400.827180539811893No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA4830.7398670383873043No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT4220.6464262737048497No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG4060.6219172206733862No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT3880.5943445360129898No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT3870.5928127201985234No Hit
ACTTTTTCCCTAGCTCTGAAGGTAGTTGATTCCCAAAGGCAGACCTTTGT3870.5928127201985234No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG3720.5698354829815263No Hit
GCAAGGTCTACCACTCTATCAACCAGGGCGACTACAGTTCCTTTATGTAG3550.5437946141355964No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC3510.5376673508777304No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG3150.48252198155693765No Hit
GCTGCCCCCACCATGAGGTCCTGCTCCATCTCGTTGTACACCTGTTGCAG2800.4289084280506112No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC2790.42737661223614476No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG2610.3998039275757483No Hit
CAGAAATGACATCTACCTGTTACTCACAGACTCTGGGTTACTCAGGCACG2590.39674029594681537No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG2540.389081216874483No Hit
GTTGATACAAAACAAGCCCACGAACTGACCAAGAGTTACGGGATTCCATT2530.38754940106001656No Hit
GGCAAGGACAGGGAAGGGGAACATCACACACCGGGGACTGCTGGGGCTGT2490.3814221378021507No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT2370.36304034802855306No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG2330.3569130847706872No Hit
ACTAGTATTCTGAGCTGTGTGCTGTGAGGGTTTGTGAATGTTGAGATGAC2320.35538126895622074No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG2230.3415949266260225No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA2160.33087221592475724No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA2140.32780858429582427No Hit
CGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT1740.26653595171716554No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT1670.25581324101590025No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC1640.25121779357250085No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC1630.24968597775803436No Hit
CATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA1620.24815416194356793No Hit
TAATGATCAAGGGGAGAAGGTGCCTCGGTACTTTCTTTTGGATTCACTGT1510.23130418798443675No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT1420.21751784565423854No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT1360.20832695076743973No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG1260.19300879262277504No Hit
CCAAAATACACCTGAATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG1210.1853497135504427No Hit
TTCTTTGTAAGGCGTGTCTCAACTATCAGGTGTGTCCAGGAAACCATTGT1180.1807542661070433No Hit
TTGTGAAGAGAACATGATTGGCAATACTCTGAAGTATCTACACAATAAAA1160.17769063447811037No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC1110.17003155540577802No Hit
CTGAGGTACTCGTCGGCATCGAGGCCAAGGTGCGTCCTGCCAACACTCTG1060.16237247633344568No Hit
GGCAAGGACAGGGAAGGGAGGATGAGTGAGCTGGGGCTGTTCCGGCTGTA1040.15930884470451273No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG1020.1562452130755798No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG1020.1562452130755798No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT980.15011794981771392No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC940.14399068655984804No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT940.14399068655984804No Hit
ATGTGTCCCAGCTAAATGTGGTCCATATGTGGTGTCCTTTGCATTCAGTA790.12101344934285102No Hit
GAGGGGGTCGTGGGACTCATGGAGCGAGGGCAGCAGTATCATCTTGTAGA730.11182255445605221No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT720.11029073864158574No Hit
ACACTTTGAATGCTCTTTCCTTCCCGGCCTATGGGTCCATCTGGGTATGT670.1026316595692534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)