FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103068
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC1146211.12081344355183No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC95939.307447510381497No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC95149.230799084099818No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG81837.939418636240152No Hit
GGTTTCTACCTGTGTGGGTGAAGAGCTGCAGAAACACAATTTGACAGACC55325.367330306205612No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCCAAAACTAGTATCTTCCTT41063.983777700159118No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT37573.6451662979780335No Hit
GCCCTCTCAGAAACATCTGCTACCGTAGACAAATCATACTTGCTGGGCAG31123.0193658555516745No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC30432.9524197617107153No Hit
TACTCTTCTTGGCTCCAGTTGCAGCAGGGAGACAGTGCTTACGCTGATGC30282.937866263049637No Hit
GCCGGAACAGCCCCAGCCATTCTCTGATGACCCGAGCTCAGACCAAGTCT29122.8253192067372996No Hit
TCTTGGTTGTCAGTGCTTCAGTATACGGTACCAGTCAAATGTCCATGGAT24412.3683393487794464No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT21392.07532890906974No Hit
CTGAAAGCTGTACCATACCTGTAAAAGCTGTTGAGCGAGCAGAGCCGAGA12771.2389878526797842No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA12681.2302557534831373No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC10100.9799355765125936No Hit
AGCTGCTCACCATCGCTACCGTAATACGGTAGCAGGTCTTGGCCAGTTGG10060.9760546435363062No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA9450.9168704156479218No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATTA7120.6908060697791749No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA6970.6762525711180968No Hit
GGTCCAGCAGCCCTTTCCGCCCGCAGTGTTCATCATTGAGACCAAGTCTC6930.6723716381418092No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA6780.6578181394807312No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT6240.6054255443008499No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA5560.5394496837039625No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA5350.5190747855784531No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC5240.5084022198936624No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC5070.49190825474444055No Hit
TCTACCCACTCTCTTTTCAGTGCTACGGTAGCAGAACTGTCAAATGACCA4710.4569798579578531No Hit
AGCTGCTCACCATCGCTATACGGTAGCAGGTCTTGGCCAGTTGGAGACCA4620.44824775876120615No Hit
AGCGTTATACCTTTGCCCTGACACTGACGACATGGTTCTACAAGAAAGAG4430.42981332712384057No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATT4420.4288430938797687No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATTAGTGG4190.4065277292661156No Hit
GGAAACCGTAGCTGCCCTGCCTGCCCAGTGCAGTTTCCAGGGGAAGACCA3520.34152210191329996No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA3500.3395816354251562No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATTAGTG3340.3240579035200062No Hit
ACTCTACCCTGCGATTTTCTCTCACACTGACGACATGGTTCTACAACTCT3150.3056234718826406No Hit
CAAAGTGGGAAGGGGAGTGAATATACGGTAGCAGAACTGTCAAATGACCA2920.2833081072689875No Hit
GCCGGAACAGCCCCAGGCTCCTCTTTGAATCTTCCTCAGGCCTCTGTCTT2740.2658439088756937No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATTAG2730.26487367563162184No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA2690.26099274265533434No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC2640.256141576434975No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC2540.2464392439942562No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC2500.24255831101796874No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATTA2450.23770714479760935No Hit
GAACACTGGGAGCCTGCACCATATGTGGCTGTGACAAGAAAGAGCAGATG2450.23770714479760935No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT2270.2202429464043156No Hit
GGTTTCTACCTGTGTGGGTGAAGCAGCATCTCTGGACTCTCATAGACAGC2260.21927271316024372No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG2200.21345131369581247No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATTAG1940.18822524934994372No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT1920.18628478286179997No Hit
CTGGTGTTGTTGGGCAGTCGTCAGTGTAGTCTGACAGAGTTCTCATTACC1890.18337408312958436No Hit
TCAGAAGCTGACATTTAAGGGAGACCAAGAGGAGAAGCAGAGAGGGAGAC1770.17173128420072187No Hit
ACACAGATTCTGAAATGAGAGCTACCGTATGGATGATGATGTTGAAATAT1750.1697908177125781No Hit
TAAAGGAACTGTAGTCGCCCTGAGCCTGGACATGGGGAGACCAAGTCTCT1670.1620289517600031No Hit
GGGATCGATGAAGGACCGGTCAGAAAGAAAGATCCAGGGCAGTTGTGGGA1510.1465052198548531No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATTAGTG1460.14165405363449374No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT1370.13292195443784685No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT1320.1280707882174875No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCAGAGAC1220.11836845577676874No Hit
TACCCTCCATGAGGCACACATAGACCAAGTCTCTGCTACCGTATTAGTGG1220.11836845577676874No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATTAGT1100.10672565684790623No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATTAGT1070.10381495711569062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)