FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103068
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG1142211.082004113788955No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG95789.292894011720417No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA95349.250203748981255No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT80237.784181317188652No Hit
GTCAAATTGTGTTTCTGCAGCTCTTCACCCACACAGGTAGAAACCTGTAG55365.371211239181899No Hit
GGTGAAATAAAGGAAGATACTAGTTTTGGACTTGGTCTAGTGAGGATGAA40373.9168316063181585No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT37413.629642566072884No Hit
GCCCAGCAAGTATGATTTGTCTACGGTAGCAGATGTTTCTGAGAGGGCTG31123.0193658555516745No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA30282.937866263049637No Hit
GGAGGAAGTCTTCTACCAGGCATCAGCGTAAGCACTGTCTCCCTGCTGCA29712.8825629681375404No Hit
GAGCTCGGGTCATCAGAGAATGGCTGGGGCTGTTCCGGCTGTAGAACCAT28932.806884775099934No Hit
ATGATCCATGGACATTTGACTGGTACCGTATACTGAAGCACTGACAACCA23162.247060193270462No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC21242.060775410408662No Hit
CGGCTCTGCTCGCTCAACAGCTTTTACAGGTATGGTACAGCTTTCAGTGT12821.2438390189001436No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT12571.2195831877983467No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG10100.9799355765125936No Hit
CCAACTGGCCAAGACCTGCTACCGTATTACGGTAGCGATGGTGAGCAGCT10070.977024876780378No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT9360.9081383164512749No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA7060.6849846703147436No Hit
CAATGATGAACACTGCGGGCGGAAAGGGCTGCTGGACCTGTAGAACCATG6960.6752823378740249No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA6910.6704311716536656No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA6750.6549074397485155No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC6290.6102767105212092No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT5390.5229557185547405No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT5250.5093724531377343No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG5060.49093802150036875No Hit
TGGTCATTTGACAGTTCTGCTACCGTAGCACTGAAAAGAGAGTGGGTAGA4670.4530989249815656No Hit
CCAACTGGCCAAGACCTGCTACCGTATAGCGATGGTGAGCAGCTTGTAGA4580.4443668257849187No Hit
CAACCTCATCTGCTCTTTCTTGTAGAACCATGTCGTCAGTGTCAGGGCAA4430.42981332712384057No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG4410.4278728606356968No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC4190.4065277292661156No Hit
TGGGAGGTGAGAGTTTAATAAAATAGTCAGGGTAGAGTTGTAGAACCATG3900.3783909651880312No Hit
TCCCCTGGAAACTGCACTGGGCAGGCAGGGCAGCTACGGTTTCCTGTAGA3510.3405518686692281No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT3460.3357007024488687No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG3410.3308495362285093No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT3370.3269686032522218No Hit
TGGTCATTTGACAGTTCTGCTACCGTATATTCACTCCCCTTCCCACTTTG2890.28039740753677184No Hit
GGCAAGGACAGGGAAGGGGCTGAAGACAGAGGCCTGAGGAAGATTCAAAG2680.2600225094112625No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG2670.25905227616719056No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT2660.2580820429231187No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT2600.2522606434586875No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA2560.24837971048239996No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG2460.23867737804168124No Hit
CAACCTCATCTGCTCTTTCTTGTCACAGCCACATATGGTGCAGGCTCCCA2450.23770714479760935No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA2420.2347964450653937No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA2260.21927271316024372No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT2130.20665968098730936No Hit
GTCAAATTGTGTTTCTGCAGCTCTAGGCTGTCTATGAGAGTCCAGAGATG2120.20568944774323744No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT1930.18725501610587186No Hit
CCCTCTCTGCTTCTCCTCTTGGTCTCCCTTAAATGTCAGCTTCTGATGTA1790.17367175068886562No Hit
TGGGTAATGAGAACTCTGTCAGACTACACTGACGACTGCCCAACAACACC1730.16785035122443434No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT1730.16785035122443434No Hit
GGAACATATTTCAACATCATCATCCATACGGTAGCTCTCATTTCAGAATC1720.16688011798036248No Hit
CCCCATGTCCAGGCTCAGGGCGACTACAGTTCCTTTATGTAGAACCATGT1650.16008848527185934No Hit
GTGTACTGCCCTCCTCCCACAACTGCCCTGGATCTTTCTTTCTGACCGGT1550.15038615283114062No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT1410.13680288741413435No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC1360.131951721193775No Hit
ATGTGTGCCTCATGGAGGGTATGTAGAACCATGTCGTCAGTGTAGATCTC1330.12904102146155935No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG1310.1271005549734156No Hit
CTGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA1220.11836845577676874No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC1100.10672565684790623No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1090.10575542360383436No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA1070.10381495711569062No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)