FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693905
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT115431.663484194522305No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT112641.6232769615437272No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG112581.6224122898667686No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC111691.6095863266585484No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC108541.5641910636182186No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT100891.4539454248059893No Hit
ATCCTGTTCCAAGTCAAGACCACATTTAGCTGGGACACATACTTCTCTTA96881.3961565343959188No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA91871.3239564493698706No Hit
GCCCTCTCAGAAACATCTGCAGGAGGACTTGGGGAAACAAATTCATATGA91331.3161744042772425No Hit
TTGAAACTACAAATGAAAGAGCTCAATTTCTTAGCATTTATAAAATAAGT91241.3148773967618046No Hit
TCTCCACTTCACCCCGTCACCACCACTTTCCAGGTTGGTTCTACTGCTGT91191.3141568370310057No Hit
GCCACACCTCACGTTAGAATTTTTGGAAGAGTGTATTTCTGGATTTAGTA89481.2895136942376837No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA89421.2886490225607252No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA87551.261700088628847No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA86911.2524769240746212No Hit
TCTCCATATCTCTCTCAATTTCAACAAGACAAACAACAGTTGGTATTAGG86211.242389087843437No Hit
GCTTCCAGTACTTCAACTTTGCCCCCTTCTACTCACTCAAAGACATCTGC85741.2356158263739272No Hit
AGGTTCAACTACGCGTGTAAATTCTACTGCAAATGCAGAGACACAAGCAA83121.1978584964800656No Hit
GATGTCAGATACCACAGCATCTTTACATTGATGTTTCTTACCTTTCCACT81631.176385816502259No Hit
TATTTAGTAGCCAGGACAGTAGAAGGACTGAAGAGTGAGAGGAGCTCCCA81481.1742241373098623No Hit
GCAACAGCTTCATTGCTGAGGGACTAGACATCACTCGCCTGTAACTTGAA80851.1651450847017963No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT79581.1468428675395046No Hit
TATTTCTGGACAGTCTACGAAAAGCTCTTGCTGGCCATGGAGGAAGTAGG79451.1449694122394276No Hit
ATTTGTTGCAGGTTTTGGTTTTAATGGCTTGTGGCGGTTTGCAGGACCGT78191.1268113070232957No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC77891.1224879486385024No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGATG77101.11110310489188No Hit
TGGGTCCAGTCAGTGAACGTAAGGGTTCTATGATTTCAGTGATGTCTTCA75711.0910715443756709No Hit
CTGTGTGTAATATTTGCGTGCTTAAATATTTTCAATGAAAAGTTACTTTG75511.0881893054524754No Hit
CTTCTTCAGGTCAGGGGAAAGGGAATGCCCAATTCAGAGAGACATGGGGG74201.0693106405055446No Hit
TGATTATTTTCATTTGGTAGGCTTGAGTTTGAAGAAACAGAAGAACCTGA73341.0569170131358039No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC72991.0518730950202118No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA71741.0338591017502397No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTC70971.0227624818959369No Hit
CGAAGGGAGAACGACGAACGTCGTGGATCAGATCGGAGCGACCGCCTGCA68830.9919225254177445No Hit
GGCCAGGGGTTGTGCTTTTTAAATTTCAATTTTATTTTTGCTAAGTATTT68680.9897608462253479No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCCAAAACTAGTATCTTCCTT68220.9831316967019981No Hit
AATGTTATTAAAGAACCGTGTGTGTTATTAACAGAGTGTCAGTTTACAAA67660.9750614277170506No Hit
CTGTGCCTCTGTGTGCTGAGAGATGTAATGACATGTAAAGGATAATTGTC64840.9344218588999935No Hit
GAAAAACAATTTATATCTGTCAGTGAATCCACTAGGACTGCTCCCACCAG63450.9143902983837845No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG63450.9143902983837845No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC62680.9032936785294817No Hit
ACTCACTCCATTGTTCTTGCTTTTGCTTTGTTTTCAGCCGACTTTGTGGT62600.9021407829602035No Hit
AGAGTCTCTTTTAAGGAGTGATTTTTGTTATTTGTTTTAAACAAAAGTGT60060.8655363486356201No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGATTTCATTTCAATTAATGCT58750.8466576836886893No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT58190.8385874147037419No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG57280.8254732276032021No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA55160.7949214950173293No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC54060.779069180939754No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC53370.7691254566547294No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGAAAATAATCACTCTATTAAAGTTT52890.7622080832390601No Hit
CCCAAGATTACTGGCATCTTCCTGGGGCTGGCTCACCTGTCGGGAAGATT50900.7335298059532644No Hit
ACATGTACATAGGGTTTATATATCATCAGCTATATGACTTATTTAATTTC48350.6967812596825214No Hit
TCTATTGTGATGGCCACGTTTTCCATTATTTTCTAATGGCAAAGATTTCT48180.6943313565978051No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT47790.6887109906975739No Hit
TATCAACTGACCCAGGCAAAATATAAATTACCTTGACAAGTTGATGAAGT46130.6647884076350509No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT46010.6630590642811336No Hit
AGAGTCAAGAACTGTTTTTAAATTGTTTGTACTATAACACCTTAATTTGA45660.6580151461655414No Hit
TGAAGAAGGAGATGGTGTGGAATTGATGGAAGCCAAATCACAGTTATTTC45520.6559975789193045No Hit
CCAGCTCACAAGAGAAGAAAATACTGCTATACGTACTCCAGAACATTTAA45000.6485037577189962No Hit
TTCCTGAGTTTTCATGGACAGCATTTCTGGATCTTCCCTGGCCCAGAGAG44110.635677794510776No Hit
GACTACAGATGTGCCACCATACCCAGCTAATTTTTTAATTTTTTGTAGAG43450.6261664060642307No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA43100.6211224879486386No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT42730.6157903459407268No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT42650.6146374503714486No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA42040.6058466216557021No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT41840.6029643827325066No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG39630.5711156426311959No Hit
GGGAAGGAAAGAATTTTGCTTAAGATATCAGTGTTTGGCCAACAATACAC39610.5708274187388763No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA38840.5597307988845736No Hit
GCTGCCCCAAAGTGTAAAGAAATGCAGAATTCTCTCAATAATGATAAAAA37760.5441667086993176No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAAAGGCACGCGATATATGT36830.5307642977064584No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC36580.527161499052464No Hit
TCGGGGGCCTACAAGAGTCCCACCAAGAGGGGGAAGAGGGAGAGGAGTTC36180.5213970212060728No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG35200.5072740504824148No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA33630.4846484749353298No Hit
AATCTGTGATTCTTAGCCAACTTGAAATGAAGACTTTTCCTTTAAATATA33410.48147801211981467No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCGGATATATGT33400.4813339001736549No Hit
GAATGAGGCAAAGTAGCCTAAAGAAAGATTGGTTCTTATCAGAAGAAGAA33180.4781634373581398No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGA32960.4749929745426247No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT30650.44170311497971626No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT27350.3941461727469899No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCAAGGTAATTAAATG26210.3777174108847753No Hit
AAGGATTTTGCTTTGACTGGCTTGTGATGTTTATCTAAATAACTTTTTTT25750.3710882613614256No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC25670.3699353657921473No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG24830.3578299623147261No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA23420.33751017790619753No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCCATATATGT22940.33059280449052825No Hit
AGCTGACAGAGACAAAGATGAAGGAAAAACAAATCACTCCTTGGGAATTA22610.3258371102672556No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG21640.31185825148975727No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC21600.31128180370511815No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA21000.30263508693553154No Hit
CTTTCCCAAAACATGGCACTCACATCTACGGAACAGGAACCTGAAGGATT19530.2814506308500443No Hit
GACCTTTTCCTCTGCCCTTATCATCGCTTTTCAGTGATAGAGGAAATGTT18200.262283742010794No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC18010.2595456150337582No Hit
ATCCTGAGTCTTATGTCTGATACCATGTTTTTGTTTTGTTTTTAGAGTCT17700.25507814470280515No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA17380.25046656242569226No Hit
TGGACATTCTAAGTTATGAGGAAACAGACATAGTTAAACACAAAATACTG16590.2390817186790699No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATACATGT16510.23792882310979166No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT15990.2304350019094833No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACGTTCTTCATACCAGGACCA15970.2301467780171637No Hit
GTCTCGGAGGCTGGCATCACTGAGGCACTGTACGGTCCTTGCAATGTCAA15680.2259675315785302No Hit
CTGACACTTACAACAGTCAAGTTCTGATAGAAGCTACAGTAATAGCACTA15340.22106772540909778No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA15090.21746492675510337No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG15030.2166002550781447No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC14770.21285334447799048No Hit
GTATTTACAGTAACATGGATATTCTCTTAGATTTTAACTAATATGTAATA14010.20190083656984747No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCAGTACATGT13820.1991627095928117No Hit
GGTATCAGATGCTTCATTACAAAACGCAAGACAAGTGTTTTCTGAAATAG13740.19800981402353346No Hit
ACAGAGTTTGAATGGATATTGTGGGTGACTTGGTCTCACCATCCAAAAGA13650.19671280650809547No Hit
TAAAGCATGAGAAAACTACTATGACTTCTAAATTACGAAAAAATGTTAAA12980.18705730611539043No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGAT12610.1817251641074787No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC12140.17495190263796917No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG11960.1723578876070932No Hit
TTTTTAGGAAGTACATCTCAGAATCTTGATTCTGGAACAGATTTGTCTTT11810.17019620841469654No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC11440.16486406640678478No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGA11260.1622700513759088No Hit
CCAAGCTACATATTGCAGAAGAGTTGACGACATGGTTCTACATTTTCTAT10620.15304688682168308No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGACACCCGGTACATGT10460.15074109568312666No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGGTACATGT10320.14872352843688977No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGATGGTT10310.14857941649073No Hit
TCTTGGTTGTCAGTGCTTCAGTAAAGCTTATTTATTTATTTTTTTCTAGC10160.14641773729833335No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGATG9740.1403650355596227No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC9150.13186243073619588No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA8970.12926841570531988No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG8740.125953840943645No Hit
AGTGGAACTGTTATTATCCCATCAAATGGCACAGCTGAGACCAAGTCTCT8550.12321571396660927No Hit
GGGAAAATTATGCTTACTAATGTGGTTTTAATTTCATCATGTTTCATATA8000.11528955692782154No Hit
TTTGAGTTGATGCACTAATAACAGGTACTTCTCTTGCCTCCTCTTTACCA7740.11154264632766735No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGATGGT7620.10981330297375No Hit
GAAAGTGGTGCATTGATGGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC7520.10837218351215225No Hit
GAAGTTCATCCTGTTTTAAGTCACACTTGTGATTTGTTAAATTTTTTAAC7470.10765162378135336No Hit
GAAAGTGGTGCATTGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTCT7180.10347237734271983No Hit
ATGAGCAAGTTGGGGTGTGCTGTGTGCATGTGTAAGAATGCGTGTGTGGG6990.10073425036568406No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)