FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693905
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT108371.5617411605335025No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA107401.547762301756004No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG106481.5345040027093046No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA105231.5164900094393325No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG99341.4316080731512237No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC96541.3912567282264863No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC92911.3389440917704873No Hit
GCAATGGTTAAGGTGAACTGGTTCTCATCTACTAGGCAGATTTCTTCAAT92451.3323149422471374No Hit
TCACAGCCATTAAGAGGACAAACTTCTACTTGCAACCCATGAATTGCTCT91781.3226594418544326No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT90991.31127459810781No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA88761.2791376341141798No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT86951.2530533718592602No Hit
TGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCT86121.2410920803279988No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC85921.2382098414048033No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA85861.2373451697278446No Hit
AGGAGCATCAGGATTCCACAAATCAATGCTATCTAACTGATGAAGAACCA85131.2268249976581809No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC84541.2183223928347542No Hit
TCACTGGAAAATTCTGAAATGAAAGGGTTTTCTTTGAATTCTCTTAGAAT81881.1799886151562533No Hit
AGAAAAAGTTTCAGTTTTACCAATTTCCATTTTTACGTTTTTAGGTGAAG80851.1651450847017963No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA80591.1613981741016421No Hit
GTTTTCTCATTCCATTTAAAGCAGTATTAACTTCACAGAAAAGTAGTGAA80381.1583718232322868No Hit
TCTCCAGGTAATAAGGGTGGGAGGGGCAAAGGAGGCAACGTTGAAGTTTT80341.1577953754476478No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG80081.1540484648474936No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT78021.1243614039385796No Hit
TGCCTGAATGTTCTTCACCACCTTCATGCAAACGTAAAGTTGGTGGTACA76831.107212082345566No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA76831.107212082345566No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAACAGAAACCTTCATTT75221.084010059013842No Hit
ATGTTACCTTAAGATCAACCACCATGGACTGAACAAGTAGGAAAATGACA75161.0831453873368833No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG74821.078245581167451No Hit
CTTACCAATACTCCATCCACAGATGAAACTTTCTCCCAAGTTAACCAAGC74311.0708958719133024No Hit
GTCACCAACCCCATGTCTTTGGAGTGTTTGGACTTATGCCGCTTGTGCTT73541.0597992520589994No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA73021.052305430858691No Hit
GTGGAAACAGACTTCCTTTTGGCCATACAAAGTGATAAAGGACTTTGATA72731.0481261844200573No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC70521.0162774443187468No Hit
GAACGCTTTGAACTTCCCGATATCCGGTCCTTCATCGATCCCGACTTGCT68470.9867344953559926No Hit
CGCGTTGCCTTTGTTTCTTCTTAATTCGCATATCCTGTATATCTCTGGCA67650.9749173157708908No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT66830.9631001361857892No Hit
GGTGAAATAAAGGAAGATACTAGTTTTGGACTTGGTCTAGTGAGGATGAA66770.9622354645088306No Hit
TCTGTTCTAACAATATAAAGCTCTTCTTCGAATGGAAGAAATAAGTTAGA64470.9290897168920818No Hit
CAAATATACCATGTGCAATACCTGTCTATAGAATCAGTCTGAAGAGTTTT64350.9273603735381645No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG62600.9021407829602035No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG62180.8960880812214929No Hit
CGAGATGTTCATTTTCCCCAAACTCACAAAGAGAATTCTTCTCGTAGACG61280.8831180060671129No Hit
CTGAATGAGAAAGTAAAAGGGGCATAAATTAAATACTTACTATATGGTTC57540.8292201382033564No Hit
TGATGGCATGGAAGAACTTTTCAGTCATCTGAAGGAGGTTCCGCTGGTTT56680.8168265108336156No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA56670.8166823988874558No Hit
GTGTCCTGCTTATTTTTCTCACATTCTTCCGTACTGGCCTGGGAACTCTC56260.8107738090949048No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA53050.7645138743776165No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA50250.724162529452879No Hit
GCAGGTGACCAACCACATAACTCGAGACAGGGACAGCGGGAGGCTCAAAC50230.7238743055605594No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT47530.6849640800974196No Hit
GGAAGTTTAACTGAAGTCTCTTTACCATTGGCTGAGCAAATAGAGTCTCC46300.6672383107197671No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCAAAACATGAATGTTCTCAAC45860.6608973850887369No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC45710.6587357058963403No Hit
GCATGCCATTCAAAGGATTTTACAAACAATGACATGAACCAAGGAACTTC45170.6509536608037123No Hit
TACCTTTGAAAACGGTCCTGCAAACCGCCACAAGCCATTAAAACCAAAAC45050.649224317449795No Hit
CTCTGACTCCACCTTTCCACTTGCCCAGTATTGTTGGTGTTTTTCTTCTT44750.6449009590650017No Hit
CCAGTACTAATGAAGTGGGCTCCTCTCTGGGCCAGGGAAGATCCAGAAAT44170.6365424661877346No Hit
ACTTGCTTTCCACTTGCTGTACTAAATCCAGAGAAAGCAGATGAATTTAC42750.6160785698330463No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT42230.6085847486327379No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC42170.6077200769557792No Hit
TCATTGGAATGGCAAAGTCTCATACTTGTAATCCTAGCGCTTTGGGAGGC41180.5934529942859613No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT41170.5933088823398016No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT40370.5817799266470194No Hit
GCAGAATTCCGAAGTTCAGCTGCATGTTGGTTTCCTTCACGACAGGTGTG40240.5799064713469423No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA39400.567801067869521No Hit
GATAAAGCTCCAGCAGGAAATGGCTGAACTAGAAGCTGTGTTAGAACAGC39220.565207052838645No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG38650.5569926719075378No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT38350.5526693135227445No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT38100.54906651486875No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA36440.5251439318062271No Hit
AATGCAGATGATTACCTCGCTCTGGCAAATGATTTCATTAAAGATTCAGA34980.5041035876668997No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC34700.5000684531744259No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT33340.48046922849669627No Hit
ATATTCAACTTACTGGTTTGTTTTCCTCATTTTGTAAAGAAGAAAACTGA31510.45409674234945707No Hit
AGGTTGCTTGTGTCTCTGCATTTGCAGTAGAATTTACACGCGTAGTTGAA31240.4502057198031431No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAGTATCACC28740.4141777332631989No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA28310.4079809195783285No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT27060.38996692630835633No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT26460.3813202095387697No Hit
GTTGATACAAAACAAGCCCACGAACTGACCAAGAGTTACGGGATTCCATT25440.36662079103047246No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGATAAAGT25310.36474733573039536No Hit
TGCACGTACCACATTTAATTACCTTGACTTGGTCTTCTCTTGGTATTAGT24640.35509183533769034No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC24260.3496155813836188No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT24190.34860679776050035No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT23210.3344838270368422No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG21470.30940834840504106No Hit
AGCATAATTTTTGGCTGCTTTGTTTTATTTAGGTTCCAGTATTACAGATA20910.30133807942009355No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA20830.3001851838508153No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACT20230.29153846708122866No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT19600.28245941447316275No Hit
AAGACTTAAATTTTACATACCTACTGTTTCATTGAATTATAATTCATCAC19280.2778478321960499No Hit
AGAAAGAAATATATGGTAAGTTTCAAGAATACATCATACCTGTATAGGGT18460.26603065261094816No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT17610.25378113718736717No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT17410.2508988982641716No Hit
CCATGGACAAGAGAAGATACTTATAGCTTCTTGTCTCCAAGCCTGTAGGT17160.2472960996101772No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG14820.21357390420878938No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC14810.2134297922626296No Hit
CCGCTAGCTGTATGAAAACCCAACAGAGTAGGTTCTTTGATCTTTTCATC14460.20838587414703744No Hit
TGAAAGAAAGTAACATTCAACACTGATACCCAAAATGAATGCACTCATTT14410.20766531441623856No Hit
GTTCTGGAGTACGTATAGCAGTATTTTCTTCTCTTGTGAGCTGGTCTGAA13870.19988326932361059No Hit
ACATGGCCTTGCTGAAGTAATTTTTGCTTCTCTTACATGCCAGTTCTCTA13750.19815392596969325No Hit
ACATGTCTTACCGAAAGGGTACACAGGTAATCGGCTCTAAAGAAACATGA13660.19685691845425526No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA13260.19109244060786418No Hit
GAAAAATAACATTCTGTGAACTACTTACGAACTGCTGGGTTGTGTCAAAT13150.18950720920010666No Hit
ACCTGGATATAGGTCTTTTGGATGGTGAGACCAAGTCACCCACAATATCC12970.18691319416923066No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA12640.18215749994595803No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG12030.17336667123021163No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC12000.17293433539173228No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG11610.167313969491501No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG11020.15881136466807416No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC10380.14958820011384844No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAA10180.1467059611906529No Hit
ATGATCCATGGACATTTGACTGGTATTTCCACTAGAAGGAATATCACATC9840.1418061550212205No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT9810.14137381918274114No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG9130.13157420684387633No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT9010.129844863489959No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCTTTGATGTCTACAAATATCAC8760.12624206483596456No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA8730.12580972899748524No Hit
CAGCTGTGCCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGT8550.12321571396660927No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTAGGCTGCAGCGACTTCGAACT7560.10894863129679135No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT7560.10894863129679135No Hit
GAGACCTACATCAGGCCTTCCATCAATGCACCACTTTCTGTAGAACCATG7520.10837218351215225No Hit
TGTGATTATTCTGGAGAGGAAGATTAAGAGGACAAGCAGATTCAAGGTGA7420.10693106405055446No Hit
GGGATTCCATGATTCGATGTTCACATCGTTCTAAATGTTTTACCATTTCT7350.10592228042743602No Hit
TTTCCAATTCCCTCCCAACCCCTCTATACTTAATATGGTAATTTCTTATA7270.10476938485815782No Hit
GAGACCTACATCAGGCCTTCATCAATGCACCACTTTCTGTAGAACCATGT7140.1028959295580807No Hit
CTGGGCGCGGGAATCCCGGCGGATCCCGGGCGGGCGGATGACCCCCAGCC6970.10044602647336451No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)