FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234988
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG111744.755136432498681No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC107984.595128261868691No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC99444.231705448788874No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC87593.7274243791172315No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT55742.3720360188605376No Hit
TTATGGAACAGACTTTATAGATTAAGGGACATCCGATGGAAGGTGGTGTA52402.229901101332834No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT51852.2064956508417453No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA41261.7558343404769603No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA35591.5145454235960987No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA32091.3656016477437145No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT29331.2481488416429776No Hit
CCAGTGTTGTGTTTCCCAAAGTTCTAGGATTACAGATGTGCCACCATACC27231.1587825761315471No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA26461.1260149454440227No Hit
GCCGGAACAGCCCCAGGCCTGGTGGGGTTCCAGACTGCTGAGGAAGACCA21010.8940882087595963No Hit
TTAGTTGCTTTTGAATTTACAGTTTAGTGAATGTGATTGATGTTTATATT14140.6017328544436312No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA13350.5681141164655217No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCGGGGCGGGGAAGAAAAG13350.5681141164655217No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA12000.5106643743510307No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA11320.4817267264711389No Hit
CTTCTTCAGGTCAGGGGAAAGTGCTGGTGTCACAGAGCTCACATACTTCC11260.4791734045993838No Hit
AACTGATCACCTTGAATCTGCTTGGAGAAGGAATATTTGATGGTCAACCA11050.47023677804824077No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC10540.448533542138322No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC9630.4098081604167021No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGCG7240.3081008391917885No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA7230.307675285546496No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGCGTCTG6670.28384428141011453No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTTCCAACACCACC6480.2757587621495566No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGC6400.27235433298721634No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT6350.27022656476075374No Hit
CTGGTGTTGTTGGGCAGTGTCAGGTTGCAAAACCCCTAATCTAGACCAAG6340.26980101111546123No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC6290.2676732428889986No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC5980.2544810798849303No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA4560.19405246225339168No Hit
TCTGATCAAAGAACAGGAGAGTTCTCATTACCCAAGAAGTCTGACAGAGT4380.1863924966381262No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC3990.1697959044717177No Hit
CTGTGGTGCCACCTAAGCTCTTACGGCAGCAAAAAGTCCTGCAACTTGTA3980.16937035082642518No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA3770.16043372427528216No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCAAGGTAATT3710.157880402403527No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGCGTCT3700.15745484875823446No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA3680.1566037414676494No Hit
GGCTTCCACTTAATTCCAGTCTGGGTGCGGAAGAGAAAGACCAAGTCTCT3500.14894377585238397No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGCG3460.14724156127121385No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC3240.1378793810747783No Hit
AGCATTGATAAGAAATCAGTTTTCCAGTGCATGTGCCAGACATCCTAATA3210.1366027201389007No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT3140.13362384462185303No Hit
CACGTTTTTCCCATCTAGCAATTCCATGCACTTCTCATTTCTGTTGAAGT3060.13021941545951282No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA3030.12894275452363524No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGCGT2950.12553832536129503No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT2940.12511277171600252No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG2900.12341055713483243No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG2790.11872946703661462No Hit
TGGTAAATTCATCTGCTTTCTCTGGATTTAGTACAGCAAGTGGAAAGCAA2780.11830391339132211No Hit
CCCATTTTCAAGAACTCTACCATGGTTTTATATGGAGACACAGGTGATAA2770.1178783597460296No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA2750.11702725245544454No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC2710.11532503787427444No Hit
CCAGTGTTGTGTTTCCCAAAGTCTTTCCTGTTGTGTAAGTATCTCCTTTT2690.11447393058368939No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT2680.11404837693839685No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA2640.11234616235722675No Hit
ACTCCAAAAAATACTAAGAGGCCCAATATATCCAGAAAATTGTGTTTTCA2640.11234616235722675No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT2640.11234616235722675No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA2630.11192060871193424No Hit
TTTCATATAGGCTGTGCAACAAGTCTCTGCTACCGTAGCTGTCCTTCAAC2600.11064394777605664No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC2600.11064394777605664No Hit
GTCTCAGAACAAACCTGAGATGCATGACTACTTCCCATAGGCTGTTCTAA2550.10851617954959403No Hit
CTGCACACTGACTCACACATTTATTTGGTTCTGTTTTTGCCTTCCCTAGA2530.10766507225900897No Hit
CCAGCTCACAAGAGAAGAAAATACTGCTATACGTACTCCAGAACATTTAA2490.10596285767783886No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG2460.10468619674196131No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG2440.10383508945137625No Hit
TATTTGTTCAGGGCTCTGTGTGACACTCCAGGTGTGGATCCAAAGCTTAT2430.10340953580608371No Hit
TTTGATGGTCAACCAGAAAGAATAAATACTGCAGATTATGTAGGAAATTA2400.10213287487020614No Hit
TGTCATAATGAATGCCCCATCGATTGGTCAGGTAGACAGCAGCAAGCAAT2380.1012817675796211No Hit
TATTGCTTGAGCTGGCTTCTTTAAAAACATTTTCTCTAATGTTATTACGG2380.1012817675796211No Hit
CGGACCCCATATAGCACAGGTACACTGACGACATGGTTCTACTCACTCAA2370.10085621393432856No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT2350.1000051066437435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)