FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234988
Sequences flagged as poor quality0
Sequence length150
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG108694.62534257068446No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT102704.370435937154237No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG99084.216385517558344No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA87323.7159344306943334No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC51812.204793436260575No Hit
TTCACCAGGTACACCACCTTCCATCGGATGTCCCTTAATCTATAAAGTCT49342.099681685873321No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT43241.8400939622448806No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG40871.7392377483105521No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT35001.4894377585238394No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT31821.3541116993208164No Hit
CTGGGTATGGTGGCACATCTGTAATCCTAGAACTTTGGGAAACACAACAC26661.134526018349873No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT26531.1289938209610704No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA25951.104311709534104No Hit
TCCTCAGCAGTCTGGAACCCCACCAGGCCTGGGGCTGTTCCGGCTGTAGA21000.8936626551143037No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG13840.5889662450848554No Hit
GGCACTTTTCTTCCCCGCCCCGCACAACTAAAAGAAAAGTTTAAAAGTGA13300.565986348239059No Hit
ATGAAAACACAACATGAATATAAACATCAATCACATTCACTAAACTGTAA13020.5540708461708682No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT11990.5102388207057382No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC11680.4970466577016699No Hit
GCTGCCCCCACCATGAGGAAGTATGTGAGCTCTGTGACACCAGCACTTTC10980.4672579025311931No Hit
GGTTGACCATCAAATATTCCTTCTCCAAGCAGATTCAAGGTGATCAGTTT10880.46300236607826784No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA10530.44810798849302946No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG9620.4093826067714096No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA7280.3098030537729586No Hit
TATGGTCCTCAGAAAAGTGCACCAGATCATCTTCCATTTGTAATACTTCT7170.3051219636747408No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC6620.28171651318365193No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCCTAATCCAT6580.28001429860248184No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG6400.27235433298721634No Hit
AGATTAGGGGTTTTGCAACCTGACACTGCCCAACAACACCAGTGTAGAAC6350.27022656476075374No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA6200.2638432600813658No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG5890.25065109707729755No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG4680.19915910599690198No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG4490.19107358673634398No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGAGATTCCATT4130.17575365550581307No Hit
TGGGTAATGAGAACTCTGTCAGACTTCTTGGGTAATGAGAACTCTCCTGT4090.17405144092464295No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG3970.16894479718113264No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTTCACCCAATT3970.16894479718113264No Hit
ACAAGTTGCAGGACTTTTTGCTGCCGTAAGAGCTTAGGTGGCACCACAGT3910.16639147530937748No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT3720.1583059560488195No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT3670.1561781878223569No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT3660.15575263417706436No Hit
TTCTCTTCCGCACCCAGACTGGAATTAAGTGGAAGCCTGTAGAACCATGT3460.14724156127121385No Hit
TGCACGTACCACATTTAATTACCTTGCAGAGACTTGGTCTTCTCTTGGTA3450.1468160076259213No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA3440.1463904539806288No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG3210.1366027201389007No Hit
ATTAGGATGTCTGGCACATGCACTGGAAAACTGATTTCTTATCAATGCTT3160.13447495191243808No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC3140.13362384462185303No Hit
CACGCACACACAAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACG3010.1280916472330502No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT2980.12681498629717264No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT2960.12596387900658756No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT2950.12553832536129503No Hit
ATATTCAAGGAGATGTCCGATTTTAAATCTTGACCTAGAGTCATTTTTAT2940.12511277171600252No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG2840.12085723526307726No Hit
CACAGTGCTCTGGGTTTCTCTTATCAACACGAGGAAGTATTTTTGATACA2810.11958057432719968No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG2800.11915502068190716No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA2800.11915502068190716No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA2790.11872946703661462No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA2760.11745280610073706No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA2690.11447393058368939No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC2620.1114950550666417No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAAGGGGCTAGAA2550.10851617954959403No Hit
AAGGGAATTGTTGAAGGACAGCTACGGTAGCAGAGACTTGTTGCACAGCC2510.10681396496842392No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT2480.10553730403254635No Hit
GTGTACTGCCCTCCTCCCACCTGTCTCTGACCAACATTGGTCTTTTGTGA2440.10383508945137625No Hit
GCTGAAGACCCCAAAGATCTCATGTTAAGTGGAGAAAGGGTTTTGCAAAC2430.10340953580608371No Hit
GCAGATGAGACTGACTTATGAAGCTTCCCTATACTACATTTACATATATC2360.10043066028903604No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA2360.10043066028903604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)