FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673226
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA164882.449103272897957No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG145732.1646519890794473No Hit
CTGTGGTGCCACCTAAGCTCTTAAGTGATAATTTATGTAGACAAACTGAA142992.1239524320213423No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT141312.098997959080606No Hit
GCCACACCTCACGTTAGAATTTTTGGAAGAGTGTATTTCTGGATTTAGCA140952.0936505720218768No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA129521.9238710329072257No Hit
CTGTATGTAGTCGGTGCTGTGACTTGTTTGTGCTCATCTCTGTTCTGTAG110481.6410536729122165No Hit
ATCCTGAGTCTTATGTCTGATACCATGTTTTTGTTTTGTTTTTAGAGTCT110021.6342209005593962No Hit
AGCAAGCTGTGACGGGTAAGCTTTATAAGGAAAGTAAATCTTCACCCCAC109631.6284278979124396No Hit
CCAGACTCTCCAGAACCAAAGGCAATCACACCACCTCAGCAACCATATAA107151.5915903426189721No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC105991.5743598732075113No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA101031.5006847626205762No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG99921.4841969858561612No Hit
GAGAAAGCCCCCCTACTGCTCACCTGGAGGGCCACTGACAACCACCCTTA98041.4562717423272424No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG94511.4038376414458147No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC93091.3827451702697162No Hit
TCCTCTTGAGATGGGTAGTTTCTATTCTGAAGACTCCCAGAGCAACTGTG89251.3257063749766052No Hit
TTTCATATAGGCTGTGCAACAAGTTATTACTAACCTGCTAATTGGGCAGA87361.297632592918277No Hit
GCACAAGCGGCATAAGTCCAAACACTCCAAAGACATGGGGTTGGTGACCC85131.264508500860038No Hit
ATTTGTTGCAGGTTTTGGTTTTAATGGCTTGTGGCGGTTTGCAGGACCGT85061.2634687311541741No Hit
CACCCTTGAACAGTAAAAGCGAATCTTTACCTGTGTCAGACAAAACTGCT84541.2557447276248987No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC84441.2542593423308073No Hit
GAAGTTCATCCTGTTTTAAGTCACACTTGTGATTTGTTAAATTTTTTAAC82101.2195013264490677No Hit
GAAAAACAATTTATATCTGTCAGTGAATCCACTAGGACTGCTCCCACCAG81931.2169761714491123No Hit
CCGAGATTCAGTTTAGGAGTTAATGTTTTATTTCAATGAAAGTAAAATAA81501.210589014684519No Hit
TTAGTTGCTTTTGAATTTACAGTTTAGTGAATTAATAATCCTTTTGTTTT80811.200339856155288No Hit
TATTTCTGGACAGTCTACGAAAAGCTCTTGCTGGCCATGGAGGAAGTAGG77331.148648447920906No Hit
TTGTGGCACCAAATACGAAACACCCATAAAGAAAAAAGAACTGAATTCTC77281.1479057552738605No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC74281.1033441964511175No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC73031.0847768802749747No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC72041.0700715658634694No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA71261.0584855605695562No Hit
CTTTTTCTGGTTGGGCAGTTGGTGGAATTAATACACTGTCTTCATTAATT70081.0409580140992774No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT69571.0333825490994109No Hit
TCTTGCTTTTTTATTTCAGGATGCTTACAATTACTTCCAGGAAGACTTTG69241.028480777628909No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC62400.9268804235130551No Hit
GGCTTCCACTTAATTCCAGTCTGGGAGCTGAACTCAGTAGAAAAAAGAAG62130.9228698832190081No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT61370.9115809549839133No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGAAAATAATCACTCTATTAAAGTTT58830.873852168513991No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT57300.8511257735143919No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGCAGTC54910.8156250649856067No Hit
GGTAGGATGTGATATTCCTTCTAGTGGAAATACCAGTCAAATGTCCATGG54030.802553674397602No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC53750.7983945955741459No Hit
TTAGCATGCCAATGTTTAAAATGTAAATGATTATTTAAAGGCAAAAGAAA52780.7839863582214591No Hit
GGGATAATGGGGTTTTCCTGTGTACGACATGGTTCTACAAGCCAGAATTT52370.7778962785156842No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT50400.7486341882220829No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGCCACCCGATATATGT49090.7291756408694852No Hit
CCTCAGGGCCTCAGAGAAAGGCAAAAGGGCCTAGACTTTGACTTCCTTTT47410.704221167928749No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA45040.669017536458782No Hit
GTTCAGCCCAGTTTGAAGCAAATGCTTTTGAAGCACCACTTACATTTGCA44240.6571344541060506No Hit
CAATTGAAGGTTTCCTTTTAACTCTAGTATCTTTGTTACACATATATGTT43660.6485192194003202No Hit
AGTTCCATTTAGGTTGCTGGTGGGAAGTTAGTGCATTTCATAACAATTTT42820.6360419829299522No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT42300.6283179794006767No Hit
ACCTTGTCACCAATACCTCACATACACTGACGACATGTTTCTTCTATAAC42000.6238618235184025No Hit
AGCCTGCTGACTACCTTAATTGGCGCTGGAATCCGAATTCTTTTCAGTTC41480.616137819989127No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC41320.6137612035185808No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC38340.5694967217546559No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAAAGGCACCTGATATATGT38010.5645949502841542No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT37680.5596931788136525No Hit
AGAGTCTCTTTTAAGGAGTGATTTTTGTTATTTGTTTTAAACAAAAGTGT34080.5062193082263609No Hit
CAAAGAGACTCACCTAATTTTCTTACATTTTGTTTTTGTAGGTAATTTTA33190.49299937910894703No Hit
GTCACTGGTTAAAACTAAGGTGGGATTTTTTTTTTAAATAGATTTAGGAC30390.45140859087438695No Hit
ACGAGATCCTAGTTACCCAACTTTCTCTGAAACCTGTGATAAAATCATTC28170.41843303734555704No Hit
ACTTCTACCGTGCCCTGACATTCCTCATTAGCAGGTCTTGGCCAGTTGGA28130.41783888322792045No Hit
AAGTCCTCCATTTCTGTATCCATGCGTTTAGGACTCAGTTCCTCTGGAAA27870.41397688146328276No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT27640.41056049528687244No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG26420.392438794698957No Hit
TCCACAAACAGAACAAGATGCTTACGGTAGCAGAAGCCTTTTTAGCCCTA26230.38961656264018324No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA25950.38545748381672723No Hit
GGCCAGGGGTTGTGCTTTTTAAATTTCAATTTTATTTTTGCTAAGTATTT25930.3851604067579089No Hit
TGCCAAAATGACGAACACAAAGGAAAAGAGGAGAGGCACCCGGTACATGT25590.38011009675799806No Hit
AAAAGCAGTGGTCCAATGTATCTGCTTGGTTCTACATCTATTGTGATGGC25450.37803055734627006No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC25040.37194047764049515No Hit
TGAAGAAGGAGATGGTGTGGAATTGATGGAAGCCAAATCACAGTTATTTC24330.36139424205244597No Hit
GAGGTAGCTTCAGAACAGCTTCAAATAAGGAAATCAAGCTCTCTGAACAT24070.3575322402878083No Hit
GCTGTTCTTTTTGGCTTCATTTGTATTACTGAGTATTTTTCTCATAGAAA23830.3539673155819888No Hit
TCTTGGTTGTCAGTGCTTCAGTAAAGCTTATTTATTTATTTTTTTCTAGC23780.3532246229349431No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT23200.3446093882292128No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC22770.3382222314646196No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAATGCGCTTTTCCCAACACCACC22620.3359941535234825No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC21250.3156443749944298No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT21230.31534729793561156No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA21190.3147531438179749No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG21030.31237652734742866No Hit
TTGTATGAACATCTGACTTTGGAAAAATCTTCAAGCAATTTAGCAGTTTC20870.30999991087688233No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGAT20440.30361275411228916No Hit
ACACAGATTCTGAAATGAGAGCTACCGTATGCTGGAAATGTTTTAGATTC20110.29871098264178747No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAAAAAAAGTTATGA19690.2924723644066034No Hit
GGAAACACTGATGGGCCTGAAAGTCACACCACCTCAGCAACCAGACCAAG18860.28014366646564454No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTTATACTTTAACAGGATTTA18530.2752418949951428No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGA18000.2673693529364582No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC16990.2523669614661347No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG15600.23172010587826378No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC15260.2266697958783529No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGATAAGA14910.22147094734903286No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC14180.2106276347021654No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATACATGT13770.2045375549963905No Hit
AAGTGGTTCTGGATTAGCTGGATTATCAGTGCGCTTTTCCCAACACCACC13070.19413985793775046No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG13050.1938427808789322No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGGCTTTTCCCAACACCAA12840.19072347176134016No Hit
TTTATCAGTGAAGGGCAAAACAGTTTTACTTTCCATCTTCTCTGTTTTGA12780.1898322405848853No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT12700.18864393234961216No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA12570.1867129314672933No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC12510.18582170029083844No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC12410.18433631499674702No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC12120.18002869764388182No Hit
GAAAGTGGTGCATTGATGGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC12090.1795830820556544No Hit
GAAAGTGGTGCATTGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTCT11800.17527546470278926No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT11730.17423569499692526No Hit
CAGTAGAGGCTAAGGATTCAGGTACACTGACGACATGGTGGTTGATCTTA11690.17364154087928868No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGATA10830.16086722735010234No Hit
AAAAGCAGTGGTCCAATGTATCTGCTTGGTTCTACGTCTATTGTGATGGC10580.15715376411487375No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGATAAG10230.15195491558555374No Hit
GTCACTGGTTAAAACTAAGGTGGGATTTTTTTTTAAATAGATTTAGGACC10060.1494297605855983No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG9340.13873498646813998No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGTCTTTGTATGCTGGCT9320.13843790940932169No Hit
AGTGGAACTGTTATTATCCCATCAAATGGCACAGCTGAGACCAAGTCTCT9310.13828937087991253No Hit
ATCCTGAGTCTTATGTCTGATACCATGTTTTTGTTTGGTTTTTAGACTCT9170.13620983146818455No Hit
TGGATCCTTTATTACGAATTGTGATCAACACTCGTTCTACATGCACCTTC8940.13279344529177423No Hit
AGGTTGACTTAGAATCTCACTTTCCTGAAGATTTTCATTCCTGCCATCAA8350.12402967205663477No Hit
ACCTTGTCACCAATACCTCACATACACTGACGACATGGTTCTACAACCTT8300.12328697940958905No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC8040.11942497764495133No Hit
GACAGCAGAACACACATACCAAAGTCAGTACTGAGCACAACAAGGAATGT7960.11823666940967818No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAT7940.11793959235085989No Hit
GAGAAAGCCCCCCTACTGCTACCGTACGGAACTGAGCGACCTCAGAGACC7690.11422612911563132No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA7570.11244366676272158No Hit
CTGTATGTAGTCGGTGCTGTGAGTAACAGGTAGATGTCATTTCTGAGACC7460.11080974293922101No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA7430.11036412735099356No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC7110.10561089440990097No Hit
GGTGCCAGTTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACCG6990.10382843205699126No Hit
AGAGTCAAGAACTGTTTTTAAATTGTTTGACACTGACGACATGGTTCCAG6890.10234304676289983No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT6740.10011496882176268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)