FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673226
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA166192.468561820250555No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG148752.2095106249610086No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC137112.0366117767287655No Hit
TCACTGGAAAATTCTGAAATGAAAGGGTTTTCTTTGAATTCTCTTAGAAT129551.9243166484954535No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT128931.9151072596720862No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT124071.8429175343792428No Hit
CCATGGACAAGAGAAGATACTTATAGCTTCTTGTCTCCAGGTCTGTAGGT112131.6655625302647254No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG103401.5358883940905432No Hit
TTCTTTGTAAGGCGTGTCTCAATATTTGACATTTTCATTTCTGTTGTAGT102571.523559696149584No Hit
GCACAAAGGCCCCATTCTGGCACTGATCTATTGCATCCCCAGCCCCAGAC102351.5202918485025831No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA102011.515241538502672No Hit
AGAAACAGCTAATAAAAAGTTCTCCAAACTTACTGATTTTTTGTTTTCCT101651.509894151443943No Hit
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG101481.5073689964439876No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG98001.4556775882096056No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT93501.388835249975491No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC90761.3481356929173858No Hit
GCCCAGCAAGTATGATTTGTCCTTTCACAATTGGTGGCGATGGTTTTCTC87431.2986723626241412No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC85491.2698558879187674No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTAGGCTGCAGCGACTTCGAACT84841.2602008835071727No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC84541.2557447276248987No Hit
TCGCTCCGATCTGATCCACGACGTTCGTCGTTCTCCCTTCGGTCTAGTTT82981.2325727170370722No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAACAGAAACCTTCATTT81011.203310626743471No Hit
ACCTGGATATAGGTCTTTTGGATGGCTGCAAGTTCTTCACCAAGCGGAAC79841.1859316188026012No Hit
TGGCTGTAATTTGGTTAGTGCTATTACTGTAGCTTCTATCAGAACTTGAC77551.1519162955679074No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA75461.1208717429213964No Hit
AGCCTCATTATATGTCCTCTTACTCTCTCACCTCAAGGTAAGCTGGGTCT74941.113147739392121No Hit
GTGTCCTGCTTATTTTTCTCACATTCTTCCGTACTGGCCTGGGAACTCTC74101.1006705029217527No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA72151.0717054896869698No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG72111.0711113355693334No Hit
ATGTTACCTTAAGATCAACCACCATGGACTGAACAAGTAGGAAAATGACA71881.067694949392923No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG71871.0675464108635138No Hit
TTACCCACAGCTCCAAACATCTATGACTGGTGTGTAACGTTCCTCTCCTA70351.044968554393324No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA68401.016003541158541No Hit
CCAGTACTAATGAAGTGGGCTCCAGTATTAATGAAATAGGTTCCAGTGAT67921.0088736917469021No Hit
TGGGAAAAGACTAAAGGAACAGGAAGAAATCAGTAGCAAAAATCCTGCTA65580.9741156758651627No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA62100.9224242676307807No Hit
ACACTTCTGGGTAACTTTTTAGACTCCAAACCTGAATCCTTAGCCTCTAC58880.8745948611610367No Hit
TCACTTTTTCTTCCTGTACACCTCTCTGATCACGAGATTTAAAACTATTA58550.8696930896905349No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGTTGTGGGTTGATTCCACACCCC56990.8465210791027085No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTATGTGAGGTATT56190.8346379967499771No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC55440.8234976070442912No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG53870.8001770579270557No Hit
GGAATCTCTGTGCTACCTTCCAGAATACTGAAACATTTGAGTTCTAAGAT52990.7871056673390511No Hit
GGAGAGCTGTAAATTCTGGCTTGTAGAACCATGTCGTACACAGGAAAACC52180.7750740464569105No Hit
ACGTTTTTAGGTGAAGCCTGTTCTTTTCCCAAAACATGAATGTTCTCAAC51810.7695781208687721No Hit
TCATTCAGAAAACAAACAGAGCACATAAAATGATACAGAAAAAGTACAGA50270.746703187339764No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC49870.7407616461633983No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG48980.7275417170459845No Hit
CTGGGTATGGTGGCACATCTGTAGTCCTAGCTGCTCAGGAGGCTGAGGTG46650.6929322396936541No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC45200.6713941529293282No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT44470.6605508402824608No Hit
TGGAGTTGAAGCCAGCTGATTATAAGATGGTTTCCTTTGTGGAGTTTTAA42470.6308431344006321No Hit
AAACACAGAAGGAATCGTCATCTATAAAACTATATGTACTATTTACAAAA41690.619257129106719No Hit
AGGCCCTGCTGACCATACATAGAAGTTCATGGACACCTCGATAACTCAGA41530.6168805126361727No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA41490.6162863585185361No Hit
GTTTCCTTCTTCCAAGCAGTACGGTCCTGTTTTATTTCACAAATCACACC39750.5904406544013452No Hit
CTGAGTAAGTGGCAAGAAAATTACCTGCGTGCTGCATCAAAGTTGCTTTT39340.5843505746955703No Hit
ACTTATATTGGGAATACTTAAATAAAACTGGAGTGCCTCACTTTTATTAC38810.5764780326368857No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG38240.5680113364605645No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT37360.5549399458725599No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTATAATGTCACC33720.5008719211676317No Hit
TGATGGCATGGAAGAACTTTTCAGTCATCTGAAGGAGGTTCCGCTGGTTT32790.48705783793258134No Hit
ACTAAAATGTTTATCTGATTAATTACCCACCTAATGCAGGAACAAGCAAG31090.4618062879330269No Hit
CCAACTGGCCAAGACCTGCTAATGAGGAATGTCAGGGCACGGTAGAAGTT27860.4138283429338736No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT27270.40506456969873417No Hit
TGGAAATATAAAAGCTGTGCTTGGCCTGACAAAGAGGAGGCTAGTTAGTA26380.3918446405813204No Hit
ACCTGATGAAGACTTTGGACCTCTTAAGCTTGAAAAAGTGAAGTCCTGCT26270.3902107167578198No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT26190.38902240852254666No Hit
AGGGCTAAAAAGGCTTCTGCTACCGTAAGCATCTTGTTCTGTTTGTGGAT26120.38798263881668266No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT25870.38426917558145407No Hit
CGCGTTGCCTTTGTTTCTTCTTAATTCGCATATCCTGTATATCTCTGGCA25540.3793674041109523No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG25030.37179193911108604No Hit
AACGTGGCCATCACAATAGATGTAGAACCAAGCAGATACATTGGACCACT24970.37090070793463115No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT23100.3431240029351213No Hit
ATGATCCATGGACATTTGACTGGTATTTCCACTAGAAGGAATATCACATC22850.3394105396998927No Hit
AGGCTTGCTCAGTTTCTTTTGATTATCTAATGCCAAGGTATTTACAATTT22750.33792515440580134No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG22560.3351029223470276No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA21780.32351691705311436No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACATAGTGAAATGTAG21230.31534729793561156No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT21060.3128221429356561No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT20950.3111882191121555No Hit
GTTCAACTCTGTGAAAATGTGATTTAGTTTTAAAAGGTGGAACAAAGACT20800.3089601411710184No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC20700.3074747558769269No Hit
TTCAATGCTTCTTTGAATCTTTGTAGGTTTACTTTTTCCTTACTAGAATC20260.3009390605829246No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA20210.3001963679358789No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG19640.2917296717595577No Hit
ACCAGAATCTAAAACATTTCCAGCATACGGTAGCTCTCATTTCAGAATCT19120.2840056682302822No Hit
GGTTGCTGAGGTGGTGTGACTTTCAGGCCCATCAGTGTTTCCTGTAGAAC18590.2761331261715977No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG17940.2664781217600033No Hit
ACAACTGTTTCATATACTTCATCTTCTAGGACATTTGGCATTGACTTTCC17260.2563775017601816No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA16820.24984180646617926No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC15460.22964056646653574No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC14920.22161948587844202No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG14200.21092471176098365No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC12960.1925059341142499No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCCTGATGTCTACAATATCACC12550.18641585440847502No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA12410.18433631499674702No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG12350.18344508382029215No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA12310.18285092970265557No Hit
CAGATCCACAAGCCCAACTTTCAACAAACAAACAAAGAGAGTTAGCTGGT12180.1809199288203367No Hit
GAGACCTACATCAGGCCTTCCATCAATGCACCACTTTCTGTAGAACCATG12100.17973162058506356No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG11960.17765208117333553No Hit
GAGACCTACATCAGGCCTTCATCAATGCACCACTTTCTGTAGAACCATGT11650.1730473867616521No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA11560.17171053999696984No Hit
ATGTTACCTTAAGATCAACCACCATGTCGTCAGTGTACCTGAATCCTTAG10920.16220407411478463No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT10820.1607186888206932No Hit
AACGTGGCCATCACAATAGACGTAGAACCAAGCAGATACATTGGACCACT10310.15314322382082687No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT10220.15180637705614458No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT9900.147053144115052No Hit
CAGCTGTGCCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGT9330.13858644793873082No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA9290.13799229382109426No Hit
CCATGGACAAGAGAAGATACTTATAGCTCCTGGTCTCCAGGTCTGTAGGT8910.1323478297035468No Hit
TAATGATCAAGGGGAGAAGGTGCATGTAGAACGAGTGTTGATCACAATTC8670.12878290499772735No Hit
GCCAACTGCCCACAAGTTCTAATTTAGAGGCAAATATTTCATGTTCTCTA8450.1255150573507262No Hit
CCCCAAAACACAGTAACCCAAATACTCACCATATTGTTAACATTCTTTAA8190.12165305558608848No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA7850.11660274558617759No Hit
AGAGACATCTTAAAGAGGGAAGCTGTATTTTTCCAGAGGAACTGAGTCCT7850.11660274558617759No Hit
CTGAGGTCGCTCAGTTCCGTACGGTAGCAGTAGGGGGGCTTTCTCTGTAG7640.1134834364685856No Hit
CAGAAATGACATCTACCTGTTACTCACAGCACCGACTACATACAGTGTAG7460.11080974293922101No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT7420.11021558882158443No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT7150.10620504852753757No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTAGAACCATGTCGTCAGT6950.1032342779393547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)