FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008099601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008099601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212478
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA175388.25403100556293No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC160127.535839004508701No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG145516.848238405858489No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT128166.031683280151357No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA103704.8805052758403225No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT76463.5984901966321217No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC71933.3852916537241504No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA70893.3363454098777288No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA69863.2878698029913687No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT58862.770169146923446No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC45622.1470458118016924No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA42662.007737271623415No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT36911.7371210195879103No Hit
AACTGAGCGACCTCAGAGCCACGACATGGTTCTACAATTTACCCTACCAA32381.5239224766799386No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG30141.4184997976261071No Hit
TGAGCAAGAGGCTTTGGAGTGCAAGACCTACACCAGTACCCCCTTCTCCC28161.3253136795338811No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC26031.225068007040729No Hit
GGTCCAGCAGCCCTTTCCCTCCTCTGTGTTGTCACTCTCTTCCTCTCTCA25791.213772719999247No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA19710.9276254482817045No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT18880.8885625805965793No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC17770.8363218780297255No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG17300.8142019409068233No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG16750.7883169081034271No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT16720.7869049972232419No Hit
TACTGAATGCAAAGGACACCACATGGTTCTACAGATTCCCGCGCCCAGAG15640.7360762055365732No Hit
GTGCAGGCTGATTTTCTTTTTCCTACGGTAGGAGACAGAAGCAGGGAAGA15400.7247809184950912No Hit
TTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAGAGGACACGTCACTTA15030.7073673509728066No Hit
TCTATGCTTGTTTCCCGACTGTGTGTGGCAGAGCAAAGGGCCCCAGACTG15010.7064260770526831No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC14290.6725402159282373No Hit
TACCCTCCATGAGGCACACAAAAAAGCCAGAATTTACAGCTCTCCAGACC14050.6612449288867553No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCCTGAAAACCAGAT12980.6108867741601484No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA12940.6090042263199014No Hit
ACCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTG12320.579824734796073No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC10550.4965219928651437No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA10130.47675524054255025No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC9270.43628046197723996No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT9050.42592644885588155No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACACTCGGTAGCA8630.4061596965332881No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATCG8610.40521842261316465No Hit
GAGAAAGCCCCCCTACTGCCCCAACTCCCTGGCTTTCAGACCAAGTCTCT8470.39862950517230017No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT7750.36474364404785437No Hit
TTACCCAGTACCATCAATGCTGTTAACAGTTCTTCATCCTTTTTCCAGCT7140.33603478948408777No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGGGA6810.3205037698020501No Hit
GCAACAGATAACTCAGATTGCCTTCGGATGAAAGGCTCCTAGACCAAGTC6410.3016782913995802No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA6230.29320682611846877No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATCGTGA5820.2739107107559371No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT5650.2659098824348874No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA5220.24567249315223225No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC5210.24520185619217047No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATC4900.2306121104302563No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC4810.22637637778970057No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA4790.2254351038695771No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC4630.2179049125085891No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC4630.2179049125085891No Hit
TCAGAAGCTGACATTTAAGGGAGCTTCTTATGGCCAAGAGACCAAGTCTC4520.21272790594790988No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATCGTGAA4430.20849217330735414No Hit
GTCACTGGTTAAAACTAAGGTGGGTACGGTAGCAGAGTCACAGTCACAGG4310.20284452978661321No Hit
GGGAAAAGAACAGGCTTCACCTATCATCGTTTTCCAAGTTATTTTTGAGA4290.2019032558664897No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC4260.20049134498630444No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATCGT4070.19154924274513127No Hit
ATTTCTCTTCTGACCTCTCAATTTTACCTTTTCTAGATGTATTCTTGGGG3960.18637223618445203No Hit
TTAAATTTCACTCCACTTACCTACCAAGGGCCTGGTTTAAGGCCCTGTAT3940.18543096226432854No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT3860.18166586658383457No Hit
TTTTTGTCCAGCCAGCAAATGAGAGTCTGTACCCGACCATTTCACAAAAG3740.1760182230630936No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC3610.16989994258229088No Hit
GTCCTCCAGAGCACAAACCTGTGGCAGATACACACTGGTCCAGCCTTAAC3330.15672210770056194No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG3210.15107446417982096No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC3210.15107446417982096No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATCC3080.14495618369901825No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG3040.14307363585877125No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG3000.14119108801852429No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATCG2930.13789662929809204No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA2920.1374259923380303No Hit
TCCCTTAGAGCTTCCACACATGGACTGGTCATTAATATCATTCACTCTCT2880.1355434444977833No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG2700.12707197921667185No Hit
TGTCATAATGAATGCCCCATCGCCTTCGGAGAGAATTACCCGAGACACGG2540.11954178785568388No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG2430.11436478129500464No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG2410.11342350737488116No Hit
TCTGGGGTATCAGGTAGGTGTCCAGCTCCTGGCACTGGTAGAGTGCTACA2410.11342350737488116No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT2340.11012904865444893No Hit
CTAACAGTACTCGGCCTGCTCGCTGGTATACCAAACTTGGATTCTTTCCT2320.10918777473432544No Hit
TCCCTTAGAGCTTCCACACATGCAACCCATTCAGCAGAATTAGACCAAGT2250.10589331601389321No Hit
AACTTGGGCATGGAGCATGTATACATGGTGTGGGTGCAGTGTGATGTCTG2230.1049520420937697No Hit
CACATCCTCCTAAACACACAGGAGGAGGAGGAGGAGGAAGAGAGAGGGAC2210.10401076817364621No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA2200.10354013121358445No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT2170.10212822033339923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)